Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	752,542	G→A	A85V (GCT→GTT)	ybgD ←	putative fimbrial protein YbgD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	752,542	0	G	A	100.0%	25.4 / NA	10	A85V (GCT→GTT)	ybgD	putative fimbrial protein YbgD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

CATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTC  >  NC_000913/752307‑752720                                                                                                                                                                                                                                            |                                                                                                                                                                                   catccaGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAACAGGCAGATcaca                                                                                                                                                                       >  1:1168/1‑249 (MQ=255)         cGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAACAGGCAGATCACAGTTGATCa                                                                                                                                                               >  1:62521/1‑249 (MQ=255)                                                  tCAAGATCTGGCGCGGCTGAACCTAATACGATGTTCCCGTCATTTTTGACCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACAGGCATTCCGCTACCGTTGTCAGAAACAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGtt                                                                                                                    >  1:92439/1‑251 (MQ=255)                                                                ggCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCCTCGCCTGCCCTGGTGTTGCTCCACCAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTCCCGTTGTCAGAAACAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTccc                                                                                                                        >  1:144362/1‑233 (MQ=255)                                                                                    nccnGTCNTNNTNNTCCNTCAGTCGTACACCGACCCTAGNNGCTTCGCCTNCACGGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGNAGGTTACGCNNACNTTGGTNANNNGNNNTCNGCTACCGTTGTCAGAAACANGCNNATCACANTTGATCAAGCGAATGTCTACGGCCAc                                                                                                                                            <  2:62521/191‑1 (MQ=255)                                                                                                           cGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAACAGGCAGATCACAGTTGATCAAGCGAATGTCGACgg                                                                                                                                                <  1:45440/166‑1 (MQ=255)                                                                                                                             gCTTCGCCTGCACTGGTGTTGATCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAACAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTc                                         >  1:62598/1‑251 (MQ=255)                                                                                                                                                 gCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTATGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCANNAacaggn                                                                                                                                                                               >  2:20691/1‑96 (MQ=255)                                                                                                                                                  cTCAACAAAGGCGTAGCACCAGTTGTCTGAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAACAGGCAGAGCACAGTTGATCAAGCGAATGGCGACGGCCACTGTATTGCTATGATGCGCCCGGTTTATATGGGTTGTCGTGAc                                                                                                  <  1:136843/173‑1 (MQ=255)                                                                                                                                                                    ccAGTTGTCTTAGCCGTGCTATCGAAGGTTATGCCAACTTTGGATACCGGCAGTCCGCTACCGTTGTCAGAAACAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTtc  <  1:20691/251‑1 (MQ=255)                                                                                                                                                                                     ncTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAACAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTatgatg                                                                                                                             >  1:27070/2‑111 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                   CATCCAGGCGAAAAAGTTCAGCGTCTGTTCTGATGAGCTTGCATCCAGGTCAAGATCTGGCGCGGCTGAACCTAATACGATGTTACCGTCATTTTTGTCCATCAGTCGTACACCGACCCCAGTTGCTTCGCCTGCACTGGTGTTGCTCAACAAAGGCGTAGCACCAGTTGTCTTAGCCGTGCTATCGAAGGTTACGCCAACTTTGGATACCGGCATTCCGCTACCGTTGTCAGAAGCAGGCAGATCACAGTTGATCAAGCGAATGTCGACGGCCACTTTATTGCTATGATGCTCCCGGTTTATATGGGTTGTCGTGACTTGTCCAAGATCTATGTTTTTATCAATATCTTCTGGATGAATTTCACAAGGTGCTTCAATAACCTCCCCCTTAAAGTGAATTTCGCCAGAACCTTC  >  NC_000913/752307‑752720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑