Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,242,121	A→G	S283P (TCG→CCG)	yeiB ←	DUF418 domain‑containing protein YeiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,242,121	0	A	G	100.0%	33.1 / NA	14	S283P (TCG→CCG)	yeiB	DUF418 domain‑containing protein YeiB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/6);  total (8/6)

CGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCG  >  NC_000913/2241879‑2242327                                                                                                                                                                                                                                                   |                                                                                                                                                                                                               cGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATGAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCTCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTGAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGc                                                                                                                                                                                                        <  1:250659/251‑1 (MQ=255)                              tAACAGAGAAGAGGATATTAGCCAGCCATACCGGAATAACAAAAGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACAAGCTTAAAGCGGCTCAATTGCGGCCAGACGCCATAAAAAAGCGGGGCATAGCCAATCGCCTGAAACGGCGCAATCAATTcc                                                                                                                                                                           >  1:22751/1‑251 (MQ=255)                                                                         cGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAAACGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTACGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGc                                                                                                                                     >  1:101907/1‑246 (MQ=255)                                                                         cGCCAGCAGCTACAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGGGGTACAAATCAGCGTTTGCAATAGAGAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGt                                                                                                                                <  1:208217/251‑1 (MQ=255)                                                                                    ccAGGCGGGCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACTCAGGCGATCGCAAGCACCAGCTGAAAGCGTCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGcc                                                                                                                                                                                                       <  1:167015/169‑1 (MQ=255)                                                                                     cAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCAttt                                                                                                                                                                 >  1:15688/1‑206 (MQ=255)                                                                                                 aTGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATgg                                                                                                        <  1:104779/251‑1 (MQ=255)                                                                                                                                                   gCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAg                                                                                                                                                             >  1:222113/1‑148 (MQ=255)                                                                                                                                                   gCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACAAGATTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTCCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCCCCCCAATAGCCACCAGCACAAAACCAGTaca                                                       >  1:176639/1‑249 (MQ=255)                                                                                                                                                                 gTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCCCTCAGTTCCCGCGGCCTTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTa                                         >  1:36573/1‑250 (MQ=255)                                                                                                                                                                     gcgcCNNCCNNNCGNNGCAGGCGATCGCAAGCACCAGCTNAAAGCGGCTCAATTGCGCCCAGAAGCCATNANANCGCGNNGCANAGCCACTAGCNTGAAACGGCGCACTCAGTTCTCGCGGCATTTGAAGTAAGAAGGCACACNANCGATACGCCCCGTCCAGCTGCAATTGCAGGGNGCTGGCAGGAAGGTTAATggagatnggaanngcngnnnn                                                                      >  2:191425/1‑198 (MQ=255)                                                                                                                                                                     gcgcCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGGTCTCGCGGCATTTGAAGTAAGAATGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATgg                                                                                         <  1:191425/198‑1 (MQ=255)                                                                                                                                                                                                       cAGCTTAAAGCGGCTCAATTGCGGCCAGCAGCCATAAAACAGCGGCGCATAGCCAATCGACTGACACGGCGCACTCAGTTCCCGCGCCATTTGAAGTACGAAGGCAGACCCGCGATACGCCCAGTCCAGCTGCCATTGCAGGGACATGGCAGGAAGGGTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATCGTGACGTAAGCTGAAATGCACTTTCAGACAGCCGCTGCGCATCcgcg  >  1:179512/1‑251 (MQ=255)                                                                                                                                                                                                                            cGGCCANNAGCCATAAAACAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATa                                                                                                                                        <  2:22751/96‑1 (MQ=255)                                                                                                                                                                                                                                            aaCAGCGGCGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGCAGTAAGAAGTCGCACCAGCGATACGCCCAGTTCAGCTGCCATTGCAGGGCGCTGGCAGGAAGGTTAATGGTCACCCCAATCGccacc                                                                        >  1:239766/1‑144 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                               CGGCCCCTGGCGGAAGTAACGCAGCCAGATAACAGAGAAGAGGATATTCGCCAGCCATACCGGAATAACAAACGCCAGCAGCTCCAGGCGGTCAAAATGCATAAACAAACCGAGGTGGTAAAAAAGCGTGGTACAAATCAGCGTTTGCAATAGATAGTTGGTCAGCGCCATCCGTCCGACGCAGGCGATCGCAAGCACCAGCTTAAAGCGGCTCAATTGCGGCCAGAAGCCATAAAACAGCGACGCATAGCCAATCGCCTGAAACGGCGCACTCAGTTCCCGCGGCATTTGAAGTAAGAAGGCACACCAGCGATACGCCCAGTCCAGCTGCCATTGCAGGGCGATGGCAGGAAGGTTAATGGTCACCCCAATCGCCACCAGCACAAAACCAGTACGACGATAGTGACGTAAGCTGAACTGCCCTTTCAGCCAGCCGCTGCGCATCAGCG  >  NC_000913/2241879‑2242327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑