Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,018,530	G→A	G824D (GGC→GAC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,018,530	0	G	A	100.0%	19.2 / NA	9	G824D (GGC→GAC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

ATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACCGCGCGTCGGGCGACCGATGCCATCCTTAGTCGGGAAAATATCCGTTCCCACCAGAACGATAAATACTGGAACAACGTCAATCCAGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTG  >  NC_000913/3018319‑3018730                                                                                                                                                                                                                    |                                                                                                                                                                                                         aTGTCGCTTGGCGAACCGGATGAGAAACGTCATAGTCGCCCGGTTGAAACACACGAAACAGCAACACCGCTTGTAGAAAGCCTGATCCCCGCCCTTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGCCAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACACGTCTGACTGACGTCTTTATGATCGACGACGTACAGCGCGGACCATCATCCCTTGTAGATGCTGt                                                                                                                                                                   >  1:220102/1‑251 (MQ=255)                                                       gAAACAGTAACACTGCTTGTAGACAGCCTGATCGCCGCCATTTGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGTCAAAAACGGCAGGCCAGACGTCGACCATAATGGCGAAACTCGGCTGACTGACGTCTTTATGATCGACGACGTACAGCGCGGACCAGCCTCCATTGTCGCTGCTGTCGGAACCGCGCGGCGGGCGACCGATGCCATCCTTAGTCGGGAAAATATCCGTTc                                                                                                             <  1:247258/251‑1 (MQ=255)                                                                                                  gTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGTCGTCTTTATGATCGACGACGTACAGCGCGGACCATCCTCCATTGTCgct                                                                                                                                                                        <  1:131888/149‑1 (MQ=255)                                                                                                                                                   cAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGACGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACCGCGCGTCGGGCGACCGATGCCATCCTTAGTCGGGAAAATATCCGTTCCCACCAGAACGATAAATACTGGAACAACGTCAATCCAGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGAc                  >  1:75377/1‑250 (MQ=255)                                                                                                                                                                 naGACGTCGACCATAATGCCGAAACTCGTCTGACTGACGTCTTTATGATCGACGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACCGCGCGTCGGGCGACCGATGCCATCCTTAGTCGGGAAAATATCCGTTCCCACCAGAACGATAAATACTGGAACAACGTCAATCCAGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGttt   >  1:204212/2‑251 (MQ=255)                                                                                                                                                                 cAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGACGCCGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACCGCGCGTCGGGCGACCGATGCCATCCTTAGTCGGGAAAATATCCGTTCCCACCAGAACGATAAATACTGGAACAACGTCAATCCAGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGttt   >  1:175834/1‑251 (MQ=255)                                                                                                                                                                 cAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGACGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACCGCGCGTCGGGCGACCGATGCCATCCTTAGTCg                                                                                                                            >  1:60789/1‑130 (MQ=255)                                                                                                                                                                 cAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCNTTAGGATCGNNGANGTACTNCNNNGNNNATNCTCCATTGGCGCTGCTGTCNGANNCGCGCGNCGGGCGACCGATGCCATCCTTAGTCg                                                                                                                            <  2:60789/130‑1 (MQ=255)                                                                                                                                                                  aGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGACGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACCGCGCGTCGGGCGACCGAAGCGATCCGTAGTCGTGAAAATATCCGTTCCCACCAGAACGATAGATACTGGAACAACGTCAAGCCAGGGGAAATCTATCGAGGTAAAGGCGATAGCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTg  <  1:233818/251‑1 (MQ=255)                                                                                                                                                                           ncATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGACGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACAGCGCGTCGGGCGACCGATGCCATCCTTAGt                                                                                                                              >  1:29449/2‑118 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                         ATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACCGCGCGTCGGGCGACCGATGCCATCCTTAGTCGGGAAAATATCCGTTCCCACCAGAACGATAAATACTGGAACAACGTCAATCCAGCGGAAATCTATCAACGTAAAGGCGATATCTCTATCACGCTGGTGAACAGTGACGATCGTGACGCGTTTG  >  NC_000913/3018319‑3018730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑