Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,378	G→A	G154G (GGC→GGT)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,378	0	G	A	100.0%	22.5 / NA	10	G154G (GGC→GGT)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

TATGCAGGCTGACGTAATAGGTATCGTCTTTTTCTTCGTAGATCAGTTTATCTTGCGATGCTGGCGTACCGATGGCGTGACGCCAGACCTGATAAGGCAGCAGCGTCACCGGATGCTTGCGAACATAGTAGAAAATCCAGGAGTCATTTGCCCAGACAAAGCTGGGTTCAACGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATGCCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCATTCCGCCCATCGAATAAAACTCACTATGAGCTGCGCGCTTATTGGCATCGAGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATAT  >  NC_000913/1928152‑1928580                                                                                                                                                                                                                                   |                                                                                                                                                                                                           tATGCAGGCTGACGTAATAGGTATCGTCTTTTTCTTCGTAGATCAGTTTATCTTGCGATGCTGGCGTACCGATGGCGTGACGCCAGACCTGATAAGGCAGCAGCGTCACAGGATGCTTGCGAACATAGTAGAAAATCCAGGAGTCCTTCGCCCAGACAAAGCTGGGTTCACCGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACTGGCGTCGGgaaagaaa                                                                                                                                                                                     >  1:246750/1‑250 (MQ=255)                          gTCTTTTTCTTCGTAGATCAGTTTATCTTGCGATGCTGGCGTACCGATGGCGTGACGCCAGACCTGATAAGGCAGTAGCGTCACCGGATGCTTGCGAACATAGTAGAAAATCCAGGAGTCATTTGCCCAGACAAAGCTGGGTTCAACGTTAGCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACTGGCGTCGGGAAAGAAAATCATCTGCCAGCGCCATAATGgtg                                                                                                                                                            <  1:61250/250‑1 (MQ=255)                                  cttcGTAGATCAGTTTATCTTGCGATGCTGGAGGACCGCTGCCGTGACGCCGGACATGATAAGGCAGCAGCGTCACCGGATGCTTGCGAACATAGTAGAAAATCCAGGGGTAATTTGCCCAGACAAAGCTAGGTTCAACGTTATCCAGAAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACCGGCGTCGGGCAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGc                                                                                                                                                   >  1:35924/1‑251 (MQ=255)                                                                          ggCGTGACGCCAGACCTGATAAGGCAGCAGCGTCACCGGATGCTTGCGAACATAGTAGAAAATCCAGGAGTCATTTGCCCAGACAAAGCTGGGTTCAACGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCATTCCGCCCATCGAATAAAACTCACTATGAg                                                                                                           <  1:60617/251‑1 (MQ=255)                                                                                                    gcagcGTCACCGGATGCTTGCGAACCTAGTAGAAAATCCAGGAGTCATTTGCCAAGACAAAGCTGGGTTAACCGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAACACGAATACCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCATTCCGCCCATCGAATAAAACTCACTATGAGCTGCGCGCTTATTGGCATCGAGCAAt                                                                                 >  1:208178/1‑251 (MQ=255)                                                                                                     cagcGTCACCGGATGCTTGCGAACATAGTAGAAAATCCAGGAGTCATTTGCCCAGACAAAGCTGGGTTCAACGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCAGGCCGCCCATCGAATAAAACTCACTATGAGCTGCGCGCTTATTGGCATCGAGCAATg                                                                                <  1:35846/251‑1 (MQ=255)                                                                                                                   gCTTGCGAACATAGTAGAAAATCCAGGAGTCATTTGCCCAGACAAAGCTGGGTTCAACGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCATTCCGCCCATCGAATAAAACTCACTATGAGCTGCGCGCTTATTGGCATCGAGCAATGTTTccc                                                                          >  1:207598/1‑243 (MQ=255)                                                                                                                                                                 gCTGGGTTCAACGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCATTCCGCCCATCGAATAAAACTCACTATGAGCTGCGCGCTTATTTGCATCGAGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCTATTGACGCTGGTATATATCATATTcac                    <  1:205239/251‑1 (MQ=255)                                                                                                                                                                      nttCAACGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATACCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCATTCCGCCCATCGAATAAAACTCACTATGAGCTGCGCGCTTATTGGCATCGAGCAATGTTTCCCCCTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCAAAGCCt               >  1:28157/2‑251 (MQ=255)                                                                                                                                                                                                                              cGAATACCGTACTGGCGCCGGGAAAGAAAATCTTCTGCCAGTGCCATAATGGAGTTATCGGACGTCCTAGCCATTCCGCCCATCGCATAAAACTCACTATGAGCTGCGCGCTTATTGGCATCGAGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAatat  >  1:167094/1‑208 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                           TATGCAGGCTGACGTAATAGGTATCGTCTTTTTCTTCGTAGATCAGTTTATCTTGCGATGCTGGCGTACCGATGGCGTGACGCCAGACCTGATAAGGCAGCAGCGTCACCGGATGCTTGCGAACATAGTAGAAAATCCAGGAGTCATTTGCCCAGACAAAGCTGGGTTCAACGTTATCCAGCAGTTCCGGGTACCAGTTACCAGTTTCCAGATTACGAAAACGAATGCCGTACTGGCGTCGGGAAAGAAAATCTTCTGCCAGCGCCATAATGGTGTTATCGGGCGTAATCGCCATTCCGCCCATCGAATAAAACTCACTATGAGCTGCGCGCTTATTGGCATCGAGCAATGTTTCCCACTCATCCCACTCTTCACTGAATGCCGATTGACGCTGGTAGATAGCATATTCACAGCCTGGTTCATAAATAT  >  NC_000913/1928152‑1928580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑