Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,610,833	G→A	V474I (GTA→ATA)	prfC →	peptide chain release factor RF3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,610,833	0	G	A	100.0%	38.2 / NA	15	V474I (GTA→ATA)	prfC	peptide chain release factor RF3
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/7);  total (8/7)

AACAGCTGCTCAAAGGGCTGGTACAGCTTTCCGAAGAGGGCGCGGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTG  >  NC_000913/4610642‑4611039                                                                                                                                                                                                |                                                                                                                                                                                                               aaCAGCTGCTCAAAGGGCTGGTACAGCTTTCCGAAGAGGGCGCGGTGCAGGTGTTCCGTCCAATCTCCAACAACGAGCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTg                                                                                                                                                     <  1:98826/251‑1 (MQ=255)        gctCAAAGGGCTGGTACAGCTTTCCGAAGAGGGCGCGGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGGAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAg                                                                                                                                                                          >  1:215996/1‑223 (MQ=255)                        cAGCTTTCCGACGAGGGCGCGGTGCCGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGACGCGAAGACATTCGAAGAGTTCAAGCGTAAGAACGAAAGcc                                                                                                                                                          >  1:74239/1‑223 (MQ=255)                             ttCCGAAGAGGGCGCGGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGTCGGCGATAACCTCGCt                                                                                                                          <  1:89449/250‑1 (MQ=255)                                          gcgGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACGACGTTGTAGCAGTGTAGGAGTCAGTCAACGTTGCCACTGCCCGCTGGAGAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACTAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAg                                                                                                            <  1:252285/251‑1 (MQ=255)                                           cgGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAATCAGTGTATGAGGCAGTCAACGTTGCCACTGCCCGCTGGATAGAAtgtn                                                                                                                                                                                                       <  1:25063/159‑2 (MQ=255)                                            ggTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGAcgc                                                                                                                                                                                                >  1:85304/1‑165 (MQ=255)                                                         cGTCGAATCTCCAACAACGATCTGATCGTTGGTGCAGGTGGTGTGCTGCAGTTTGACGTGGTGGGAGCGCGCCTGAAGACCGAATACAGCGTCGAAACAGCGTTAGACTCATTCAATGTTGCCACTGCCCGCTGGATATAATGTGCAGACGCGAAGAAATGCGAAGATTTGAAGCCTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTATATc                                                                                                                    <  1:141224/228‑1 (MQ=255)                                                                                                                    ggaggtAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCt                                  >  1:4488/4‑251 (MQ=255)                                                                                                                     gtggtAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTACGCCTGGCACAGGAACGTTATcc                                                                        <  1:124070/212‑1 (MQ=255)                                                                                                                                               cAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTgcg       >  1:38730/1‑251 (MQ=255)                                                                                                                                                         gCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGATCGAATGTGAAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTCGCGCTTGATGGCGGCGATAACCTCACTTACATCGCTCCCAGCATGGTCAACCTGCACCTGGCACAGCAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTACTTCTTGCCTCCAGGGCCCGGTAGCCGCTGCGCCCTg  >  1:112380/1‑246 (MQ=255)                                                                                                                                                                        gtcaACGTTGCCCCTGCCCGCTGGATAGAATGTGCAGACGAGAAGAAATTCGACGAGTTCAAGCGTACGAACGAAAGCCAACTGGCGCTTGATGGCGGCGCTAACCTCGCTTACATCACTACCAGCATGGTCAACATGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAgga                       >  1:145991/1‑209 (MQ=255)                                                                                                                                                                              gTTGCCCCTGCCCGCTGGATAGAATGTGCAGACGCTAAGAAATTCGAAAAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTgcgc                                                                                            <  1:1884/135‑1 (MQ=255)                                                                                                                                                                                           gCTGGATAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAg                                                     >  1:163430/1‑161 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                               AACAGCTGCTCAAAGGGCTGGTACAGCTTTCCGAAGAGGGCGCGGTGCAGGTGTTCCGTCCAATCTCCAACAACGATCTGATCGTTGGTGCAGTTGGTGTGCTGCAGTTTGATGTGGTGGTAGCGCGCCTGAAGAGCGAATACAACGTTGAAGCAGTGTATGAGTCAGTCAACGTTGCCACTGCCCGCTGGGTAGAATGTGCAGACGCGAAGAAATTCGAAGAGTTCAAGCGTAAGAACGAAAGCCAACTGGCGCTTGATGGCGGCGATAACCTCGCTTACATCGCTACCAGCATGGTCAACCTGCGCCTGGCACAGGAACGTTATCCGGACGTTCAGTTCCACCAGACCCGCGAGCATTAATTCTTGCCTCCAGGGCGCGGTAGCCGCTGCGCCCTG  >  NC_000913/4610642‑4611039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑