Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,755,646	G→A	noncoding (54/363 nt)	ssrA →	tmRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,755,646	0	G	A	100.0%	25.0 / NA	10	noncoding (54/363 nt)	ssrA	tmRNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/8);  total (2/8)

CACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCG  >  NC_000913/2755408‑2755863                                                                                                                                                                                                                                               |                                                                                                                                                                                                                          cACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTGGCCTc                                                                                                                                                                                                               <  1:8715/251‑1 (MQ=255)          tccctcTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACGTACCTTTACACATTGGGGCTGATGCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTggn                                                                                                                                                                                                                  <  1:50930/239‑2 (MQ=255)                                                            caAATGTTGCCATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTGGCCTCGTAAAAAg                                                                                                                                                                                                       >  1:79731/1‑200 (MQ=255)                                                                         cccATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAAGCGGGTATACTTGCCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAATGTGCATGCCAAGGAGCGGTTGTCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTGCGCGTTAGCAGCTTAATAACCTGCTTAGAGc                                                                                                                                        <  1:221437/250‑1 (MQ=255)                                                                                  tAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCctctctn                                                                                                                                <  1:51873/249‑2 (MQ=255)                                                                                         aTAAAAAGCAGGCTACATGGGTGCGAAATCTTTAACGATAACGCCATTGAGGCTGGTCAGTGCGCTCAGAAATCTGGTATACTGACCTTTAGACAGTGGGGCTGAGTCTGGATGCGACGGGATATGCGAAACCGAAGGTGCATGCCGAGGAGCGGTGAGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGTCGAAAACTACGCGTTAGCAGCTTAATAACCTGCTTAGGGCCCTCTCTCCCTAGCCTc                                                                                                                       <  1:109381/251‑1 (MQ=255)                                                                                                                                  cGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTagcagn                                                                                                                                                           <  1:76300/174‑2 (MQ=255)                                                                                                                                                              aaaTCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGtt                                                   <  1:93661/250‑1 (MQ=255)                                                                                                                                                                                            ggCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGt                    >  1:3408/1‑251 (MQ=255)                                                                                                                                                                                                              cGGGATTTGCGAAACCCAAGGTGCATGCCGAGGAGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCGGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGtccgtccg  <  1:101005/251‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                          CACCGCGCCTCCCTCTCCGGCGGCGCGAATGAACATCTTATTGGCTATCACATCCGACACAAATGTTGCCATCCCATTGCTTAATCGAATAAAAATCAGGCTACATGGGTGCTAAATCTTTAACGATAACGCCATTGAGGCTGGTCATGGCGCTCATAAATCTGGTATACTTACCTTTACACATTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCATGCCGAGGGGCGGTTGGCCTCGTAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACGCTTTAGCAGCTTAATAACCTGCTTAGAGCCCTCTCTCCCTAGCCTCCGCTCTTAGGACGGGGATCAAGAGAGGTCAAACCCAAAAGAGATCGCGTGGAAGCCCTGCCTGGGGTTGAAGCGTTAAAACTTAATCAGGCTAGTTTGTTAGTGGCGTGTCCGTCCG  >  NC_000913/2755408‑2755863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑