Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,496,246	G→A	intergenic (‑37/‑159)	ahr ← / → leuX	NADPH‑dependent aldehyde reductase Ahr/tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,496,246	0	G	A	100.0%	31.5 / NA	13	intergenic (‑37/‑159)	ahr/leuX	NADPH‑dependent aldehyde reductase Ahr/tRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/6);  total (7/6)

CCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGG  >  NC_000913/4496024‑4496477                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        nccGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAgtg                                                                                                                                                                                                             >  1:63696/2‑251 (MQ=255)                                                      naTCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCg                                                                                                                                                        >  1:31716/2‑251 (MQ=255)                                                                                                catcgtggggCCTCAGGTCACCGGGTTCGTACTCATAAACGTCCAGTCCGCCGCCCTCTTCGGTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTTGGAAGTGTAAATCATGGCAGGGCGGGGTGCCGTGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACGTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAgg                                                                                                              <  1:241329/243‑1 (MQ=255)                                                                                                                    ccGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGc                                                                                                                                              <  1:11577/199‑1 (MQ=255)                                                                                                                                                cgccgcCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGNTCCNTCTCNGGNGTTGTTTGGGAAGTGNAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACANNTNNNTNTATGCAAt                                                                                                                                                                               <  2:181088/138‑1 (MQ=255)                                                                                                                                                cgccgcCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGCAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAAt                                                                                                                                                                               >  1:181088/1‑138 (MQ=255)                                                                                                                                                                         gCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACCAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGAGCAAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGACGTGGCGAAATCGGTAGACGCAGTTGATTCaa                                      >  1:187028/1‑250 (MQ=255)                                                                                                                                                                                                ggTCCTTCTCTGGTGTTGTTTGGGAANTGTAAANNATNGCAGGGCGGGGTGCCTGGANTGNNNCNNNGNTTACANATNNCTGTATGCANTGCTGAAAATTTCAGCACTTAGCGAg                                                                                                                                                      >  2:56332/1‑115 (MQ=255)                                                                                                                                                                                                ggTCCTTCTCTGGTGTTGTTTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAg                                                                                                                                                      <  1:56332/115‑1 (MQ=255)                                                                                                                                                                                                 ntCCTTCTCTGGTGTTGTTTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACTTCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAACTCAACCGTAGAAATACGTGCCgg             >  1:52802/2‑251 (MQ=255)                                                                                                                                                                                                 gTCCTTCTCTGGTGTTGTTTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGAGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCAc                                                                                                                                                              >  1:243344/1‑106 (MQ=255)                                                                                                                                                                                                            gggttgttTGGGAAGTGTAAAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGGTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAAGTGCGAGCAAGCTGGCGCTTGCATGGTGGAGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGGGCCGAAGGGGCGAAAACGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCgg  <  1:253081/249‑1 (MQ=255)                                                                                                                                                                                                                 gttTGGTAAGGGTAAAGCATGGCAGGACGGGGTGCCTGGAGTGTGACAAGTGGTACGCATCGCTGTGTGGAATGCTGAGAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCAtggtgg                                                                                                                         <  1:123176/127‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                        CCCGGCAACCAGCGGATATTGTGAAAATCCCCATTCGTTATCGATCATCGACAGATCGGAATGGCAGATCCCGCAGTAATCCACCTGCACTTCAACATCTTGTGGCCTCAGCTCACCGGGATCGTACTCATAAACTTCCAGTTCGCCGCCCGCTTCTTTTGCGGCATAGCTTTTTATCATCGACATTTTTTGGTCCTTCTCTGGTGTTGTTTGGGAAGTGTAGAGCATGGCAGGGCGGGGTGCCTGGAGTGTGACAAAGGTTACACATCGCTGTATGCAATGCTGAAAATTTCAGCACTTAGCGAGGTGCGAGCAAGCTGGCGCTTGCATGGTGGCGTGCGACAGGTATAATCCACAACGTTTTCCGCATACCTCTTCAGTGCCGAAGTGGCGAAATCGGTAGACGCAGTTGATTCAAAATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGG  >  NC_000913/4496024‑4496477

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑