Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,912,831	G→A	I662I (ATC→ATT)	prc ←	tail‑specific protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,912,831	0	G	A	100.0%	23.8 / NA	11	I662I (ATC→ATT)	prc	tail‑specific protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

ACAAAACGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGTAAT  >  NC_000913/1912600‑1912994                                                                                                                                                                                                                                        |                                                                                                                                                                    aCAAAAAGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTATGCATAGTGCGTAAGTCACCAGAAAGACACACATTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAACATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAATATTCACCGTCTCATcc                                                                                                                                                   >  1:39786/1‑250 (MQ=255)                                       cgcgACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGatc                                                                                                             >  1:74408/1‑250 (MQ=255)                                                                                         cTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTGCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCgttcgttc                                                            <  1:123788/249‑1 (MQ=255)                                                                                                                        tCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAAGATTCACCGTCTCATCCAGATAAg                                                                                                                                            <  1:94593/138‑1 (MQ=255)                                                                                                                                            cAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTACGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAAt                                                                                                                          >  1:162928/1‑136 (MQ=255)                                                                                                                                                 acaATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCCGGTTGTTCCGCGGCTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAATATTCACCGTCTAATCCAGATAAGGAACCGGCTCCTGGTAATCTTTCGGTAGATCATACAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCCCAGCGTAAt  >  1:187857/1‑251 (MQ=255)                                                                                                                                                              ccTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAATATTCACCGTCTCATCCAGATAAGGATcc                                                                                                                                       >  1:92288/1‑105 (MQ=255)                                                                                                                                                                                          gttgttCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCAATATTCACCGTCTCATCCAGATAAGTATCNGGCNCCTGNTANTCTGTCGGTAGATCANCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGNNCNNNCNCGCCAGAc                                               <  2:177984/165‑1 (MQ=255)                                                                                                                                                                                          gttgttCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCAAGTGCAATATTCAAAGTCCTATAAACCTAACGATCCGGCTCCTGGTACTCTTTCGGTAGATCATCAAGTTTCTTCCACTCCGGTTTACCTTCGCGTTTACAGCGTTCGTTCAAACGCGCAAGAc                                               >  1:177984/1‑165 (MQ=255)                                                                                                                                                                                                          gtcTTTTTCATGCTTCGCCAGATCGAGTGCAATTTTCACCGTCTCAGCGAGATAAGGTTCCGTCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTTTTCAACTCCGGTTTACCTTCGCTGTTAATGCGTTCGTTCATACGCTCCATACGCTTCGCATCATCTTCATTATTCTCGTTCTCa               <  1:168215/179‑1 (MQ=255)                                                                                                                                                                                                                          gCCAGATCGAGTGCAATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGcgt                                                                  >  1:61987/1‑114 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                    ACAAAACGCAATATGCGATACCCACATCGTATGGGTTACGCGACTATTTTCAAGTCTGGATAGTGCGTAAGTCACCAGAAAGACACAACTTTACATTTTGTAGCATCTGATTTACGGCATCTTGTCGCTGTTAAAAAATCAGGCACAATTTCTTGTGCCTGATTGATATTACTTGACGGGAGCGGGTTGTTCCGCGGGTCTGGCTTTTTCAAGCTTCGCCAGATCGAGTGCGATATTCACCGTCTCATCCAGATAAGGATCCGGCTCCTGGTAATCTTTCGGTAGATCATCCAGTTTCTTCAACTCCGGTTTACCTTCGCGTTTAAAGCGTTCGTTCAAACGCGCCAGACGCGTCGCATCATCTTCATTATTCTCTTTCTCACGCACAGCGTAAT  >  NC_000913/1912600‑1912994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑