Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,064,539	G→A	E183K (GAA→AAA)	scpB →	methylmalonyl‑CoA decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,064,539	0	G	A	100.0%	15.0 / NA	8	E183K (GAA→AAA)	scpB	methylmalonyl‑CoA decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/1);  total (7/1)

CATTTGAAATGATCATGAGTTCCGATCTGATCATCGCCGCCAGTACCTCAACCTTCTCAATGACGCCTGTAAACCTCGGCGTCCCGTATAACCTGGTCGGCATTCACAACCTGACCCGCGACGCGGGCTTCCACATTGTCAAAGAGCTGATTTTTACCGCTTCGCCAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCAACCATGTTGTGGAAGTGGAAGAACTGGAAGATTTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAATTTCGTTGGT  >  NC_000913/3064324‑3064772                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          cATTTGAAATGATCATGAGTTCCGATCTGATCATCGCCGCCAGTACCTCAACCTTCTCAATGACGCCTGTAAACCTCGGCGTCCCGTATAACCTGGTCGGCATTCACAACCTGACCCGCGACGCGGGCTTCCACATTGTCAAAGAGCTGATGTTTACCGCTTCGCCAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCAACCATGTTgtgaaagtg                                                                                                                                                                                                                                      <  1:141066/221‑1 (MQ=255)                             natcatcGCCGCCAGTACCTCAACCTTCTCAATGACGCCTGTAAACCTCGGCGTCCCGTATAACCTGGTCGGCATTCACAACCTGACCCGCGACGCGGGCTTCCACATTGTCAAAGAGCTGATTTTTACCGCTTCGCCAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCAACCATGTTGTGAAAGTGGAAGAACTGGAAGATTTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGt                                                                                                                                                                            >  1:77539/2‑251 (MQ=255)                                                                                     cGTATAACCTGGTCGGCATTCACAACCTGACCCGCGACGCGGGCTTCCACCTTGTCAACGAGCTGATTTTTACCCCTTCGCCAATCACCGCCCAGCGCGCGCTGGCCGACGACATCCTCACCCATGCTGTGAAAGTCGACGAACTGGAAGATTTCACCTTCCAACTGGCGCACCCCATCTCTGAGACAGCCCCGTTAGCCCTTGCCGTTATCAAAGAAGAGCTGAGTGAACTGGCCGAAGCACACACCATg                                                                                                                    >  1:176595/1‑251 (MQ=255)                                                                                               ggTCGGCATTCACAACCTGACCCGCGACGCGGGCTTCCACATTGTCAAAGAGCTGATTTTTACCGCTTCGCAAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCAACCATGTTGTGAAAGTGGAAGAACTGGAAGATTTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATg                                                                                                          >  1:165195/1‑251 (MQ=255)                                                                                                    gCATTCACAACCTGACCCGCGACGCGGGCTTCCACATTGTCAAAGAGCTGATTTTTACCGCTTCGCCAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCAACCATGTTGTGAAAGTGGACGAACTGGANGATNNNNNCtnnnnnn                                                                                                                                                                                                          >  2:153606/1‑144 (MQ=255)                                                                                                                                      caTTGTCAAAGAGCTGATTTTTACCGCTTCGCCAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCAACCATGTTGTGAAAGTGGAAGAACTGGAAGATTTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATg                                                                   >  1:225032/1‑251 (MQ=255)                                                                                                                                          gTCAAAGAGCTGATTTTTACCGCTTCGTCAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCCACCCTGTTGTGAAAGCGGAAGAACTGGAAGATTTCACCTTACAAATGGCGCACCACATCTCTg                                                                                                                                                                                        >  1:117317/1‑130 (MQ=255)                                                                                                                                                                                            ngctgTCGGCATCCTCAACCATGTTGTGAAAGTGGAAGAACTGGAAGATTTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCt              >  1:152267/2‑250 (MQ=255)                                                                                                                                                                                                        ccTCAACCATGTTGTGAAAGTGGAAGAACTGGAAGATTTCTCCTTACAAATGGCGCACCACATCTCGGCGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAACCGTAAACCTAATTTCGTTggn  <  1:153606/250‑2 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                          CATTTGAAATGATCATGAGTTCCGATCTGATCATCGCCGCCAGTACCTCAACCTTCTCAATGACGCCTGTAAACCTCGGCGTCCCGTATAACCTGGTCGGCATTCACAACCTGACCCGCGACGCGGGCTTCCACATTGTCAAAGAGCTGATTTTTACCGCTTCGCCAATCACCGCCCAGCGCGCGCTGGCTGTCGGCATCCTCAACCATGTTGTGGAAGTGGAAGAACTGGAAGATTTCACCTTACAAATGGCGCACCACATCTCTGAGAAAGCGCCGTTAGCCATTGCCGTTATCAAAGAAGAGCTGCGTGTACTGGGCGAAGCACACACCATGAACTCCGATGAATTTGAACGTATTCAGGGGATGCGCCGCGCGGTGTATGACAGCGAAGATTACCAGGAAGGGATGAACGCTTTCCTCGAAAAACGTAAACCTAATTTCGTTGGT  >  NC_000913/3064324‑3064772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑