Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	828,038	C→T	G315D (GGC→GAC)	ybhF ←	ABC exporter ATP binding subunit YbhF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	828,038	0	C	T	100.0%	24.8 / NA	11	G315D (GGC→GAC)	ybhF	ABC exporter ATP binding subunit YbhF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

TCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGCCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAGCAAATCAATAAACGCATCTTCAAAACGCGGCGTAGTTTCGTTGATGTTGATTTCCGGCATCCCGTCGGCATGGCGAATATCGTCTGGTGTGGCCTCTTTTTTGAGGATCAGACGTACCGATTTCCCCTGAATCATGCCGTCGCTGACCTGCGGCAGTTTCAAGGCGCGTTGCAACAGTTTG  >  NC_000913/827802‑828253                                                                                                                                                                                                                                             |                                                                                                                                                                                                                        tCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCGAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCTATCACCGGCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTc                                                                                                                                                                                                            <  1:249825/250‑1 (MQ=255)    gATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCATCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCGTGGGCAGTTCTTTCGCTTCGATCACCGGCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAg                                                                                                                                                                                                        <  1:250710/251‑1 (MQ=255)              cAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTGCCGGCAc                                                                                                                                                                                              <  1:70111/251‑1 (MQ=255)                     aGCGCCTGGCCGGAAGTCGGCCCCAGCAAACCGCCCATCATCTTAAAGGTGGTCGATTTCCCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCATTTCTTTCGCTTCGATCACCGTCTCTCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAg                                                                                                                                                                                       <  1:145614/251‑1 (MQ=255)                                                                                       ccGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTACCGGCAAAGTTGACGTGATCGGTGGCGGCCAAATCCCCAACTTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTTCCGGCCCCGCCCAGCAAATCAATAAACGAATCTTCAAAACGCGGCGTAGTTTCGTTGATGTTGATTTCCGGCATCCCGTc                                                                                                                     >  1:172344/1‑251 (MQ=255)                                                                                       ccGTTTGGCCCCAGCAAACCAAAAAGCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGGGGCGGCAAAATCCCCAAATGACTTGGTCAGTTCTTTCTCTGCGATCACCGTCGCGCCTGGTGTGTCTTCTACCGTATGTAATAGTGCGTCCAGCGGCTATTCCGAGGTTGCTGCCCCTCCCAGCAAATCAAGAAACGCATCTTCAAAACGCGGCGTAGTTTCgttgatg                                                                                                                                         <  1:252185/231‑1 (MQ=255)                                                                                        cGTTTGGCCCCAGCAAACCAAACATCTCACCACGTTTAACGGCAAAGTTGACGAGATCGGTGGCGGCAAAATCCACAAATTTCTTGGACAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAGCAAACAAATAAACGAATCTTCAAAACGCGACGTAGTTGCGATGATGTTGATTTCCGGCATCCCGTCg                                                                                                                    >  1:214627/1‑251 (MQ=255)                                                                                                                                                tCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAGCAAATCAATAAACGCATCt                                                                                                                                                                    >  1:5108/1‑147 (MQ=255)                                                                                                                                                                                                          cGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAGCAAATCAATAAACGCATCTTCAAAACGCGGCGGAGTTTCGTTGATGTTGATTTCCGGCATCCCGTCGGGATGGCGAATATCGTCTGGTGTGGCCTCTTTTTTGAGGATCAGACGTACCGATTTCCCCTGAATCATGCCGTCGCTGACCTGCGGCAGTTTCAAGGCGCGTTTCAACAGTTTg  <  1:59838/251‑1 (MQ=255)                                                                                                                                                                                                           gCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAGCAAATCAATAAACGCATCTTCAAAACGCGGCGTAGTTTCGTTGATGTTGATTTCCGGCATCCCGTCGGCATGGCGAATATCGTCTGGTGTGGCCTCTTTTTTGAGGATCAGACGTACCGATTTCCCCTGAATCATGCCGTCGCTGACCTGCGGCAGTTTCaan                    <  1:151122/232‑2 (MQ=255)                                                                                                                                                                                                           gCCGGGTGTGCCTTCTACCGTATGTAATATTGCGTCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAGCAAATCAATAAACGCATCTTCAAAACGCGGCGTAGTTTCGTTGATGTTGATTTCCGGCATCCCGNCGGNNNNNCgnnnnnn                                                                                                      >  2:151122/1‑144 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                        TCAGATCCATCCCCAGCACCAGCGCCTGGCCGGAAGTCGGCACCAGCAAACCGCACATCATCTTAAAGGTGGTCGATTTACCCGCGCCGTTTGGCCCCAGCAAACCAAAAATCTCCCCACGTTTAACGGCAAAGTTGACGTGATCGGTGGCGGCAAAATCCCCAAATTTCTTGGTCAGTTCTTTCGCTTCGATCACCGTCTCGCCGGGTGTGCCTTCTACCGTATGTAATATTGCGCCCAGCGGCGATTCCGAGGTTCCGGCACCGCCCAGCAAATCAATAAACGCATCTTCAAAACGCGGCGTAGTTTCGTTGATGTTGATTTCCGGCATCCCGTCGGCATGGCGAATATCGTCTGGTGTGGCCTCTTTTTTGAGGATCAGACGTACCGATTTCCCCTGAATCATGCCGTCGCTGACCTGCGGCAGTTTCAAGGCGCGTTGCAACAGTTTG  >  NC_000913/827802‑828253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑