Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,135,180	IS2 (–) +5 bp	coding (1346‑1350/2181 nt)	katG →	catalase/hydroperoxidase HPI

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4135180 =	0 (0.000)	9 (0.890)	7/298	0.2	100%	coding (1346/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4135184	0 (0.000)	16 (1.580)	12/298	0.0	100%	coding (1350/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134935‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCG  <  NC_000913/4499511‑4499274                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          GAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATACTGAGTTCGAGAAGATCTGTCGTCGTTTCCTCAACGATCCGCCGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACTCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGACAGAAGATATGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGCCATTT                                                                                                                                                                                                                                               >  1:170621/1‑251                                                      AGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATAGGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTGAACCCATTACAAGCC                                                                                                                                                                                          <  1:200267/251‑1                                                                           GTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGCCCGAGCAGGACATTTG                                                                                                                                                                                                                                              >  1:146055/1‑178                                                                                                                 nCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTCCATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCC                                                                                                                                                                                                                                    >  1:27576/2‑150                                                                                                                                     GACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCn                                                                                                            <  1:159859/250‑2                                                                                                                                        GCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGCAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTT                                                                                                                            >  1:169462/1‑231                                                                                                                                                     GGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATA                                                                                                                         >  1:71907/1‑221                                                                                                                                                                           GGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTAn                                                                                                                                                                                               <  1:65404/129‑2                                                                                                                                                                                                         GGCAAGATCCGCTGCCGCAGCCGATCTACAACCCNACCNNGCNNGANATTTG                                                                                                                                                                                                                                              <  2:146055/52‑1                                                                                                                                                                                                           CAAGATCCGCTGCCGCACCCGATCTAGAACCCGACCGACCACGACATTTGGATTTGCCCCTATACTGCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGT                                                                                                                                                                     <  1:228891/123‑1                                                                                                                                                                                                            AAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGATCTATAACCCAGCGCACTAGGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCn                                                                              <  1:47890/209‑2                                                                                                                                                                                                                      GCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAG                                                                                                                                                    >  1:18517/1‑129                                                                                                                                                                                                                           AGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCAAGACCTCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTC                                                                                                >  1:234329/1‑176                                                                                                                                                                                                                                        CCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAAACCATTACAAGCCCGCTGCCGCCGATATTCCCGTGGCGAGCGATAACCCAGCGCAATATGCGGATGCCATTCGTTATACTGCTCGACCGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC        >  1:34002/1‑251                                                                                                                                                                                                                                              CGAGCAGGACATTTGTATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATCCCCGTGGCGAGCGATAACCCAGCTCACGATGCTGATGCCATTCGTTATAAGTCTCGAACGCCGCTGCAAGGTGCTTTGCTGCCGTTAACCCGTCTGGTTGTGGCATGATATTTATTTAGTCACGCTGGGTCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCG  <  1:146930/251‑1                                                                                                                                                                                                                                                   AGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCGGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTTCTATTCCGT           <  1:207274/237‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          GAAACGTAAACCGACAATGCTGGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134935‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCG  <  NC_000913/4499511‑4499274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.