Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,721,243	G→A	G431R (GGA→AGA)	patZ →	peptidyl‑lysine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,721,243	0	G	A	100.0%	27.0 / NA	13	G431R (GGA→AGA)	patZ	peptidyl‑lysine N‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/5);  total (8/5)

TATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGC  >  NC_000913/2721106‑2721434                                                                                                                                          |                                                                                                                                                                                                tATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGACCTGCTGCGGCGAGCACTCCTCGCAAGAGGAAAGAAGTTTATTCAGCGAAGCCGGGCTGCCGCCATACCGTACCACGGCAAGCACCATCACTGCTTTTATGCATCTGGTGGAGTACCGGCGTAATCAGAAGCAACTCCGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCCATACCGCAGCAGCGCAtctt                                                                                >  1:244033/1‑251 (MQ=255)           aGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCGCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTAACGTACCCAGGAAAGAACCATCACTGCTTTGATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAAGCTGACTTCCAATGCCGCAGAAGCGCATCTTCTGTTGCAAc                                                                      <  1:211944/251‑1 (MQ=255)                                   caAATATGTCTATTAGATGAAGAACTGGTGCGGCGCGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGACGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGACGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGt                                              >  1:90059/1‑251 (MQ=255)                                                                                gCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGCAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGc  >  1:146851/1‑250 (MQ=255)                                                                                             cgTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCCGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAAt                                                                                                              >  1:137588/1‑129 (MQ=255)                                                                                                      aGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAgg                                                      <  1:59509/176‑1 (MQ=255)                                                                                                      aGCGAAGCCGGGCTGCCGACCTACCGNACCCCGNNAANAACCATCACTGCTTTTATGNATNNNGNNNANTACCGNCGNNATCAGAAGCNACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGNGCATCTTCTNNTGCCACAGGCGATTGCCGAAgn                                                      >  2:59509/1‑175 (MQ=255)                                                                                                                    nccGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGCAACGCCGGCGTTGCCCAGCACTCTGACTTCCAATACCGCAGAAGCGCAtctt                                                                                >  1:23917/2‑136 (MQ=255)                                                                                                                    gCCGACCTCCCGTACCCCGGAAAGACCCATCACTGCTTTTATGCATATGGGGAAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGc                                                                                                                               <  1:11374/89‑1 (MQ=255)                                                                                                                    gCCGACCTACCGTACCCCGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACgn                                                                                                                               >  2:11374/1‑88 (MQ=255)                                                                                                                           tACCGTACCCCGGAAAGAACCATCACTGCTTGTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCTTTGCCCAGCAATCTGACTACCAATACCGCAGAAGCGCATCTTCTGttn                                                                          <  1:31789/135‑2 (MQ=255)                                                                                                                             ccGTACCCCGGAAAGAACCATCACTGCGTTTATGCATATGGTGGAGTACCGGCGTAATCAGAATCAACCACGCGAAACGCCGGCGTTGCCCAGCAATCTTGCTTCCAATACCGCAGAAGCGCATCTTCTTTTGCAACAGGCGATTGCCGAAGGGGCTACGGCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGc         <  1:127155/198‑1 (MQ=255)                                                                                                                                   nccGGAAAGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGcc                     >  1:159878/2‑180 (MQ=255)                                                                                                                                          |                                                                                                                                                                                                TATTAATTGAAGCGGTAAAGCATCATCCCCGCAGCAAATATGTCTCTTTGCTGACGAACTGGTGCGGCGAGCACTCCTCGCAAGAGGCACGACGTTTATTCAGCGAAGCCGGGCTGCCGACCTACCGTACCCCGGAAGGAACCATCACTGCTTTTATGCATATGGTGGAGTACCGGCGTAATCAGAAGCAACTACGCGAAACGCCGGCGTTGCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGC  >  NC_000913/2721106‑2721434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑