Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,225,963	C→T	A81V (GCG→GTG)	ebgC →	DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,225,963	0	C	T	100.0%	16.4 / NA	7	A81V (GCG→GTG)	ebgC	DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

GCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTGGAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAG  >  NC_000913/3225781‑3226058                                                                                                                                                                                       |                                                                                                ncAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTGGAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGTGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCt                             >  1:64954/2‑251 (MQ=255)                                                gCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTGGAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGTGcc                                                                                              <  1:71635/139‑1 (MQ=255)                                                     gCCCACTCCATCGGTGACTCATTGACTTACCGCGTTGAGACAGTCTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTGTGAAGTGCAGTACTACCTGCAAGGGCAGCAAAAAATTGAATATGTGCCGAAAGAGACATTACAGGTAGTGTAat                                                                    <  1:240112/160‑1 (MQ=255)                                                                 ggTGACTCATTGACTTACCGCGTGGAGACAGACTCCGCGCCCGATGCGATATTTACCGGGCATCGACGCTATTTTGAAGGGCATTACTACCTGCAAGGGCAGCAAAAACTTGAATATGTGCCGAAAGCGACATTACAGGTAGTGGAATATTATCGTGCTGCAACt                                                   >  1:170987/1‑165 (MQ=255)                                                                                             cagacTCCGCGACCGATGAGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGAAAAAAATTGAATATGTGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAg  >  1:90537/1‑186 (MQ=255)                                                                                                                                    gCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGTGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTc         >  1:130238/1‑140 (MQ=255)                                                                                                                                            gAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGTGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAggn                              <  1:29398/111‑2 (MQ=255)                                                                                                                                                                                       |                                                                                                GCAACGCTGCGTTGAAGCGATTGAAAATATCGACAACATTCAGCCTGGCGTCGCCCACTCCATCGGTGACTCATTGACTTACCGCGTGGAGACAGACTCCGCGACCGATGCGCTATTTACCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAG  >  NC_000913/3225781‑3226058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑