Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,483	C→T	L290L (CTG→TTG)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,483	0	C	T	100.0%	24.6 / NA	10	L290L (CTG→TTG)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

GGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTGCCTATACCTTTGAACCAGGTATGGATTTTG  >  NC_000913/2813282‑2813699                                                                                                                                                                                                          |                                                                                                                                                                                                                         ggTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATACTTACCGTGGCGGCCGTCCTGGCTATCTGCTTCCTGATTGTCAGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCACCTTCCCCATCGGCTGCTTGTGTATCAGCCTCGCGTATCTGCTCTACTTCGGCGCTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACgg                                                                                                                                                                                   >  1:146052/1‑241 (MQ=255)  gTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTACGGCGCTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGc                                                                                                                                                                         >  1:83663/1‑250 (MQ=255)            ggACTGGTTTTCATCACAGGAAATTATCATCCTTAACGTGGTGGCGGTGGTGGCTTTCTGTTTCCTGATTGTCTGGGGGCTGACAGAAGATAACCCGTTAGTCGATCTGTCGTTGTTTACGCCGCGCAACTTCACCATCGGTTGCTTGTGTTTCAGCCTCGCGTATATGCTCTCCTTCGGCGCTATTGTTTTGCTGCCGCCGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCct                                                                                                                                                              >  1:209503/1‑251 (MQ=255)                                                                                                                             tgttTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGTCGACCTGTGCAGGGTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGcggcgg                                                                                 <  1:178375/215‑1 (MQ=255)                                                                                                                             tgttTAAGNCNNNCNNATTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTNCTTCGGCGCTATTGTNTTNCTGCNGCANTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGCTCCTGTCGCCGATTATCGGCCGCTTCGCGNATANAATGGNNANgcggcgnnnaannnnnnnncnnnnggn                                                             >  2:178375/1‑214 (MQ=255)                                                                                                                                    gTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCTCGGATAAACTGGATATGcggcgg                                                                                 <  1:83718/208‑1 (MQ=255)                                                                                                                                      cgcgCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTg                                                                                                                                                                  <  1:166863/125‑1 (MQ=255)                                                                                                                                            aCTTCACCATCGGCTGCTTGTGTATCAGCCTCGGGTATATGCTCTACTTCGGCGCTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCGCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGAGATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTGcc                              <  1:174678/251‑1 (MQ=255)                                                                                                                                                                        ccTCGCGTATATGCTCTACTTCGGCGCTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTGCCTATACCTTTGAACCAGGTATGGATTTTg  >  1:221713/1‑251 (MQ=255)                                                                                                                                                                                                 gcTATTGTTTTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTCTCGGCCGCTTCGCGCATAAACTGGATATgcggcg                                                                                  >  1:75614/1‑146 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                         GGTAAAGAGCTGGACTGGTTTTCATCACAGGAAATTATCATCCTTACCGTGGTGGCGGTGGTGGCTATCTGCTTCCTGATTGTCTGGGAGCTGACCGACGATAACCCGATAGTCGATCTGTCGTTGTTTAAGTCGCGCAACTTCACCATCGGCTGCTTGTGTATCAGCCTCGCGTATATGCTCTACTTCGGCGCTATTGTTCTGCTGCCGCAGTTGTTGCAGGAGGTCTACGGTTACACGGCGACCTGGGCAGGTTTGGCCTCTGCGCCGGTAGGGATTATTCCGGTGATCCTGTCGCCGATTATCGGCCGCTTCGCGCATAAACTGGATATGCGGCGGCTGGTAACCTTCAGCTTTATTATGTATGCCGTCTGCTTCTACTGGCGTGCCTATACCTTTGAACCAGGTATGGATTTTG  >  NC_000913/2813282‑2813699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑