Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	861,220	C→T	A463T (GCC→ACC)	ybiW ←	putative pyruvate formate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	861,220	0	C	T	84.6%	21.2 / ‑5.0	13	A463T (GCC→ACC)	ybiW	putative pyruvate formate lyase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/6);  minor base G (2/0);  total (7/6)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAATAAATTCCGGGATCACGATTTCGTCGTTGTTGAACGCCGGCATCCCGAAGCCGCAACGGATCACCTGTACGCAGGCGTCGAGGAAATCGTTG  >  NC_000913/860990‑861428                                                                                                                                                                                                                                       |                                                                                                                                                                                                                 caccaGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGTGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGTGAAGTTGATAAAGCTCATgn                                                                                                                                                                                              <  1:54310/251‑2 (MQ=255)                                               gtgtCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGCTCTGCGAATCCCACGCGTCCATAACTTCATAGACGCTATTGAACTTACCTGCCGACAACGCATTTTCTTATGGCAGGCACACTTTCCCGCTGGTGGCATCATGCCCGCCTTCCAGCCCCGCCAGCATCACGCGGGTGAAGTTGATCAAGCTCCTGCCGGGACAGCAATAGCCACATTTGCCACAGCCGGAGGTTTTTATAc                                                                                                                                                 >  1:182569/1‑250 (MQ=255)                                                                               gCGGGTGAAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCAAGCGGGTGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAATCATAAACGTCCttcg                                                                                                                >  1:72536/1‑248 (MQ=255)                                                                                                       aTCCCACGCGTCCTTCACTTCGTCAAAGTTGTTGAATTTACCTGCCGACAACGCTTTTTCTTGGGGCATGAACACTGTGCCGCTGTTGCGATCATTCCCGCCTTCCAGCCCCGCCAGCATCACGCGGGTGAAGTTGATAAAGCGCATGCCGGTACAGCGATAGCCCCAGTTTCCACCGACGGCGGTTTCTATACa                                                                                                                                                <  1:221764/195‑1 (MQ=255)                                                                                                                               gAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCAGCATGCCCTCCTTCCAGCGCCGCCAGCATCACGCGGGTGAAGTTGATAAAGCTCATGCCGGGACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCTTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAATAAATTCCGGGATCACGATTTCGTCgttgt                                                                <  1:216737/251‑1 (MQ=255)                                                                                                                                              aCCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCCCGCGGGTGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGGCCTGCGGTTCAATACCGAGTTTAATAAATTCCGGGATCACGATTTCGTCGTTGTTGAACGCCGGCATc                                                  <  1:164890/250‑1 (MQ=255)                                                                                                                                                                    tgtgGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCCCCCGGGGGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGGTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTtaata                                                                                            >  1:119572/1‑186 (MQ=255)                                                                                                                                                                                           ggtggCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGTGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTGCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTGTAATAAATTCCGGGATCACGATTTCTTCGTTGTTGAACGCCTGCATCCCTAAGCCGCAACGGATCACCTGTACGCAGGCGTCGAGGAAAtn      <  1:28365/249‑2 (MQ=255)                                                                                                                                                                                             tggCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGTGAAGTTGATACAGCTCATGCAGGTACAGCGCTAACCCCATTTGCCACCGACGGCGGCTTCTATACAACCAATAGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATTCCGAGTTTAATAAATTCCGGGATAACGATTTCGTCGTTGTTGAACGCCGGCATCCCGAAGCCGCAACGGATCACCTGTACGCCGGCGTCGAGGAAATCGTTg  >  1:249881/1‑251 (MQ=255)                                                                                                                                                                                                      gCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGTGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCa                                                                                                                             <  1:6162/119‑1 (MQ=255)                                                                                                                                                                                                      gCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGGGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCNNTTTCTANNc                                                                                                                                                 >  2:6162/1‑99 (MQ=255)                                                                                                                                                                                                       nccGCCTTCCAGCGCCGCCAGCATCACGCGGGTGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAATAAATTCCGGGATCCCGATTtcgtc                                                                     >  1:158015/2‑174 (MQ=255)                                                                                                                                                                                                           ccTTCCAGCGCCGCCAGCATCACGCGGGTGAAGTTGATAAAGCACATGCCGGTACAGCGTTAGCCCCATTTgac                                                                                                                                                                     >  1:34591/1‑72 (MQ=255)                                                                                                                                                                                                                                   nnggNNNNNTTGNTAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAATAAATTCCGGGATCACGATTtcgtc                                                                     <  2:158015/144‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                 CACCAGCGCCGAGCAGAGAATATCGTGCACGTTCTCTTCCAGCATGGTGTCGACGACATATTCGATTTCGATTGATTTGCGGGTGTAGTAACGGATTTGCGTATCCCACGCGTCCATCACTTCATCGAAGTTGTTGAAGTTACCTGCCGACAACGCTTTTTCTTGTGGCAGGAACACTTTGCCGCTGGTGGCATCATGCCCGCCTTCCAGCGCCGCCAGCATCACGCGGGCGAAGTTGATAAAGCTCATGCCGGTACAGCGATAGCCCCATTTGCCACCGACGGCGGTTTCTATACAACCAATCGCTGCGTAGTCATAAGCGTCCTGCGGTTCAATACCGAGTTTAATAAATTCCGGGATCACGATTTCGTCGTTGTTGAACGCCGGCATCCCGAAGCCGCAACGGATCACCTGTACGCAGGCGTCGAGGAAATCGTTG  >  NC_000913/860990‑861428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑