Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,911,729	C→T	L641L (CTG→CTA)	relA ←	GDP/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,911,729	0	C	T	100.0%	24.0 / NA	10	L641L (CTG→CTA)	relA	GDP/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/5);  total (5/5)

AGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTACGGCTGGCAACGCCAAGCACGTTCACCTTCTCGTTGGCGAGAATGGTCGTGATATCACGTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCCAGTTGTTCGCAATCGGCGCGGTGTACTGAAATACCGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACT  >  NC_000913/2911487‑2911950                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              aGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTACGGCTGGCAACGCCAAGCACGTTCACCTTCTCGTTGGCGAGAATGGTCGTGATATCACGTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGTTGTTc                                                                                                                                                                                                                       >  1:73519/1‑251 (MQ=255)                                                                     gCTGGNNACNNNAANNACGTTCACCTTCTCGTTGGCGAGNATGGTCGTGATATCACGTAACAACCCACTNCNANCATTNNCTANCACGCGGACCNCCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGTCAACAANGNGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGNTGTTCg                                                                                                                                                                                                                      >  2:185690/1‑184 (MQ=255)                                                                     gCTGGCAACGCCAAGCACGTTCACCTTCTCGTTGGCGAGAATGGTCGTGATATCACGTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGTTGTTCg                                                                                                                                                                                                                      <  1:185690/184‑1 (MQ=255)                                                                                 aaGCACGTTCACCTTCTCGTTGGCGAGAATGGTCGTGATATCACGTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGTTGTTCGCAAGCGGCGCGGTGTACTTAAATACCGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGgcagn                                                                                                                                       <  1:29517/251‑2 (MQ=255)                                                                                                   gTTGGCGAGAATGGTCGGGATATCACGTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGGCAACAATGCGTGCTGGCGCATGGGAGCGCAGTTCCGCTAGTGGTTCGCAATCGGCGCGGTGGACTGAAATACCGCGCCCCTGGGTAATGAAGGCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCa                                                                                                                     <  1:94868/251‑1 (MQ=255)                                                                                                       ncGAGAATGGTCGTGATATCACGTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGTTGTTCGCAATCGGCGCGGTGTACTGAAATACCGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAgcgcg                                                                                                                                 >  1:51311/2‑235 (MQ=255)                                                                                                                             gTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCTGCGGAGTAGCTCTCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCACAGTTCCGCTAGTTGTTCg                                                                                                                                                                                                                      <  1:41022/128‑1 (MQ=255)                                                                                                                                                                                          nctctCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGTTGTTCGCAATCGGCGCGGTGTACTGAAATACCGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTgcgg                                                           >  1:29751/2‑222 (MQ=255)                                                                                                                                                                                                 ccccATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGTTGTTCGCAATCGGCGCGGTGTACTGAAATACCGCGCCCCTGGGTAATGAAGCCGACAatctcatn                                                                                                                                                           <  1:75738/119‑2 (MQ=255)                                                                                                                                                                                                                      cGTTCTGGCGCATGGGAGCGCAGTTCCGCTAGTTGTTCGCAATCGGCGCGGTGTACTGAAATACCGCGCCCCTGGGTAATGAAGCCGCCAATCTCATCTCCAGGAATCGGCTGGCAGCACCGCGCGCTGTGGTGCATCAGGTTGCCAACACCTTCGCCTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACt  >  1:33921/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                              AGCACTTGCAGGTTGTAAATCTCAATGGTCATGTCGATGGTCGCCAGTTGCTGTTTGGTGTCGCTACGGCTGGCAACGCCAAGCACGTTCACCTTCTCGTTGGCGAGAATGGTCGTGATATCACGTAACAACCCACTACGATCATTAGCTACCACGCGGACCACCAGCGAATATCCGGCGGAGTAGCTCTCACCCCATACCGCGTCAACAATGCGTTCTGGCGCATGGGAGCGCAGTTCCGCCAGTTGTTCGCAATCGGCGCGGTGTACTGAAATACCGCGCCCCTGGGTAATGAAGCCGACAATCTCATCTCCAGGAATCGGCTGGCAGCAGCGCGCGATGTGGTGCATCAGGTTGCCAACACCTTCGACTACCACGCGACCGTTATCTTTACTGCGGTTTTGCGGCGTGTAGCTTTTTTGCTGAAGTTGCTTCAGCGCGGCGGCGTCCTGCTCTTCGGCACT  >  NC_000913/2911487‑2911950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑