breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC JC 57,276 IS2 (+) +5 bp intergenic (‑167/‑84) lptD ← / → djlA lipopolysaccharide assembly protein LptD/co‑chaperone protein DjlA
RA 68,465 C→T Q528Q (CAG→CAA araB ← ribulokinase
RA 71,134 G→A G250S (GGT→AGT)  araC → DNA‑binding transcriptional dual regulator AraC
RA 74,996 G→A T162I (ACA→ATA)  thiB ← thiamine ABC transporter periplasmic binding protein
RA 154,417 G→A S337F (TCC→TTC)  htrE ← putative fimbrial usher protein HtrE
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 276,792 G→A E26E (GAG→GAA mmuM → homocysteine S‑methyltransferase
RA 283,868 G→A C223Y (TGT→TAT)  yagF → D‑xylonate dehydratase
RA 286,109 G→A A239T (GCG→ACG)  yagG → putative D‑xylonate transporter YagG
RA 286,492 G→A L366L (CTG→CTA yagG → putative D‑xylonate transporter YagG
RA 294,754 C→T G16R (GGA→AGA)  yagM ← protein YagM
RA 296,801 G→A A99V (GCC→GTC)  intF ← putative phage integrase
RA 344,389 G→A A72T (GCT→ACT)  yahL → uncharacterized protein YahL
RA 345,165 G→A intergenic (+174/‑239) yahL → / → yahM uncharacterized protein YahL/uncharacterized protein YahM
RA 383,799 G→A A46V (GCG→GTG)  yaiP ← putative glucosyltransferase
RA 384,894 G→A intergenic (‑278/‑338) yaiS ← / → tauA putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein
RA 467,730 G→A D107N (GAC→AAC)  cof → HMP‑PP phosphatase
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
RA 563,802 G→A G158D (GGT→GAT)  sfmF → putative fimbrial protein SfmF
JC JC 568,449 IS2 (–) +5 bp coding (135‑139/333 nt) emrE → multidrug/betaine/choline efflux transporter EmrE
RA 603,168 C→T Q404* (CAG→TAG)  pheP → phenylalanine:H(+) symporter PheP
RA 606,147 G→A A18V (GCT→GTT)  ybdJ ← DUF1158 domain‑containing protein YbdJ
RA 615,785 C→T R543R (CGC→CGT entF → apo‑serine activating enzyme
RA 616,477 C→T P774L (CCG→CTG)  entF → apo‑serine activating enzyme
RA 627,548 G→A L493L (CTG→CTA entE → 2,3‑dihydroxybenzoate‑AMP ligase
RA 628,224 G→A A177A (GCG→GCA entB → enterobactin synthase component B
RA 629,709 G→A G137E (GGA→GAA)  entH → proofreading thioesterase in enterobactin biosynthesis
RA 631,633 G→A W580* (TGG→TGA cstA → pyruvate transporter CstA
RA 671,691 C→T D166N (GAT→AAT)  lptE ← lipopolysaccharide assembly protein LptE
RA 672,395 C→T E797K (GAA→AAA)  leuS ← leucine‑‑tRNA ligase
RA 677,723 C→T C103C (TGC→TGT djlB → J domain‑containing protein DjlB
RA 678,229 C→T A272V (GCA→GTA)  djlB → J domain‑containing protein DjlB
RA 680,493 G→A Q94Q (CAG→CAA djlC → co‑chaperone DjlC
RA 690,588 C→T A98A (GCG→GCA lnt ← apolipoprotein N‑acyltransferase
RA 690,713 C→T A57T (GCC→ACC)  lnt ← apolipoprotein N‑acyltransferase
RA 694,841 C→T E39K (GAA→AAA)  miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 723,625 C→T E264K (GAA→AAA)  kdpD ← sensor histidine kinase KdpD
RA 754,777 C→T R2H (CGT→CAT)  ybgU ← protein YbgU
RA 757,445 C→T C513C (TGC→TGT sdhA → succinate:quinone oxidoreductase, FAD binding protein
RA 757,538 C→T D544D (GAC→GAT sdhA → succinate:quinone oxidoreductase, FAD binding protein
RA 759,605 C→T G300G (GGC→GGT sucA → subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
RA 778,541 C→T L268L (CTG→TTG)  tolB → Tol‑Pal system periplasmic protein TolB
RA 778,850 C→T L371L (CTG→TTG)  tolB → Tol‑Pal system periplasmic protein TolB
RA 779,360 C→T N98N (AAC→AAT pal → peptidoglycan‑associated outer membrane lipoprotein Pal
RA 779,557 C→T S164F (TCC→TTC)  pal → peptidoglycan‑associated outer membrane lipoprotein Pal
RA 794,007 C→T G213D (GGC→GAC)  modE ← DNA‑binding transcriptional dual regulator ModE
RA 797,058 C→T P169S (CCT→TCT)  modC → molybdate ABC transporter ATP binding subunit
RA 801,374 C→T L206L (CTG→TTG)  ybhH → putative isomerase YbhH
RA 813,075 C→T intergenic (+128/‑451) bioD → / → uvrB dethiobiotin synthetase/UvrABC excision nuclease subunit B
RA 814,630 C→T P369S (CCA→TCA)  uvrB → UvrABC excision nuclease subunit B
RA 817,542 C→T R167C (CGT→TGT)  moaA → GTP 3',8'‑cyclase
RA 828,572 C→T G137D (GGC→GAC)  ybhF ← ABC exporter ATP binding subunit YbhF
RA 839,883 G→A S550F (TCT→TTT)  fiu ← iron catecholate outer membrane transporter Fiu
MC JC 878,329 Δ29,995 bp [bssR][ltaE] 36 genes
RA 969,142 A→G V259V (GTA→GTG lpxK → tetraacyldisaccharide 4'‑kinase
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,095,492 G→A intergenic (‑55/‑351) insE4 ← / → ycdU IS3 element protein InsE/uncharacterized protein YcdU
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,290,881 C→T L214F (CTT→TTT)  rssB → regulator of RpoS
MC JC 1,294,035 Δ141 bp [tdk] [tdk]
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,344,613 G→A intergenic (‑4/‑34) yciZ ← / → ymiD UPF0509 family protein YciZ/protein YmiD
RA 1,346,924 G→A intergenic (+24/+54) ymiB → / ← rnb putative protein YmiB/RNase II
RA 1,358,227 G→A R321C (CGT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,359,428 G→A intergenic (‑87/+62) ymjE ← / ← puuA protein YmjE/glutamate‑putrescine ligase
RA 1,359,466 G→A intergenic (‑125/+24) ymjE ← / ← puuA protein YmjE/glutamate‑putrescine ligase
RA 1,394,087 G→A P287P (CCG→CCA mppA → murein tripeptide ABC transporter periplasmic binding protein
RA 1,406,261 G→A V95M (GTG→ATG)  smrA → DNA endonuclease SmrA
RA 1,408,820 G→A Q257Q (CAG→CAA zntB → Zn(2(+)):H(+) symporter
RA 1,409,541 C→A P11T (CCT→ACT)  dbpA → ATP‑dependent RNA helicase DbpA
RA 1,494,388 Δ1 bp coding (684/924 nt) ydcI ← DNA‑binding transcriptional dual regulator YdcI
RA 1,517,508 G→T V40V (GTG→GTT ortT → orphan toxin OrtT
RA 1,582,293 C→T G78R (GGA→AGA)  ydeN ← putative sulfatase YdeN
RA 1,650,209 C→T intergenic (+224/+2519) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,665,014 C→T A170V (GCA→GTA)  dmsD → redox enzyme maturation protein DmsD
RA 1,667,887 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,669,453 G→A A47V (GCC→GTC)  ynfL ← putative DNA‑binding transcriptional regulator YnfL
RA 1,670,201 G→A G168D (GGC→GAC)  ynfM → putative transporter YnfM
RA 1,698,593 G→A T196T (ACC→ACT malI ← DNA‑binding transcriptional repressor MalI
RA 1,707,546 C→T L210F (CTT→TTT)  rsxC → SoxR [2Fe‑2S] reducing system protein RsxC
RA 1,708,108 A→G Y397C (TAC→TGC)  rsxC → SoxR [2Fe‑2S] reducing system protein RsxC
RA 1,712,731 C→T intergenic (+573/‑38) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,715,932 C→T intergenic (‑43/+16) pdxY ← / ← tyrS pyridoxal kinase 2/tyrosine‑‑tRNA ligase
RA 1,726,995 C→T L113L (CTG→TTG)  nemA → N‑ethylmaleimide reductase
RA 1,734,541 C→T A36V (GCT→GTT)  mepH → peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH
RA 1,744,751 Δ1 bp coding (1295/1374 nt) mdtK → multidrug efflux pump MdtK
RA 1,751,127 C→T A200T (GCT→ACT)  ydhW ← uncharacterized protein YdhW
RA 1,753,839 C→T intergenic (‑9/+12) ydhV ← / ← ydhY putative oxidoreductase YdhV/putative 4Fe‑4S ferredoxin‑like protein YdhY
RA 1,761,385 C→T S136N (AGC→AAC)  sufD ← Fe‑S cluster scaffold complex subunit SufD
RA 1,786,384 C→T V243V (GTG→GTA ppsA ← phosphoenolpyruvate synthetase
RA 1,789,682 C→T P24S (CCT→TCT)  ydiE → PF10636 family protein YdiE
JC JC 1,792,772 IS2 (–) +5 bp intergenic (‑41/+33) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,794,855 C→T G100R (GGG→AGG)  btuC ← vitamin B12 ABC transporter membrane subunit
RA 1,794,878 C→T G92D (GGC→GAC)  btuC ← vitamin B12 ABC transporter membrane subunit
RA 1,810,624 C→T I138I (ATC→ATT ydjM → inner membrane protein YdjM
RA 1,852,006 C→T C40C (TGC→TGT pncA → nicotinamidase
RA 1,861,743 C→T D78N (GAT→AAT)  yeaC ← DUF1315 domain‑containing protein YeaC
RA 1,866,085 C→T G130S (GGC→AGC)  mipA ← MltA‑interacting protein
RA 1,871,049 C→T S222F (TCT→TTT)  cdgI → putative c‑di‑GMP binding protein CdgI
RA 1,874,299 C→T intergenic (‑117/‑53) yoaI ← / → yeaL protein YoaI/DUF441 domain‑containing inner membrane protein YeaL
RA 1,876,474 C→T L268F (CTT→TTT)  nimT → 2‑nitroimidazole exporter
RA 1,876,658 C→T S329F (TCT→TTT)  nimT → 2‑nitroimidazole exporter
RA 1,877,096 C→T T63I (ACC→ATC)  yeaO → DUF488 domain‑containing protein YeaO
RA 1,883,309 C→T A41V (GCC→GTC)  yeaV → putative transporter YeaV
RA 1,893,087 C→T G50D (GGC→GAC)  yoaA ← putative ATP‑dependent 5' to 3' DNA helicase
RA 1,978,243 G→T intergenic (‑46/+275) flhD ← / ← insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
RA 2,003,264 C→T E115K (GAA→AAA)  fliC ← flagellar filament structural protein
RA 2,026,760 C→T A420T (GCT→ACT)  dgcQ ← diguanylate cyclase DgcQ
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,072,358 G→A G274S (GGC→AGC)  flu → self recognizing antigen 43 (Ag43) autotransporter
RA 2,095,666 G→A G181D (GGC→GAC)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
RA 2,098,971 C→T D215N (GAT→AAT)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 2,187,922 C→T A165T (GCA→ACA)  yehA ← putative fimbrial adhesin YehA
RA 2,226,317 G→A V173M (GTG→ATG)  yohD → DedA family protein YohD
RA 2,226,323 G→A G175S (GGC→AGC)  yohD → DedA family protein YohD
RA 2,242,121 A→G S283P (TCG→CCG)  yeiB ← DUF418 domain‑containing protein YeiB
JC JC 2,269,876 IS5 (+) +4 bp intergenic (+309/‑100) lpxT → / → mepS lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
RA 2,291,951 T→C I154V (ATT→GTT)  ccmH ← holocytochrome c synthase CcmH component
JC JC 2,319,850 IS2 (+) +5 bp coding (23‑27/2850 nt) rcsC ← histidine kinase RcsC
RA 2,323,892 C→T A149V (GCC→GTC)  atoD → acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
RA 2,363,025 C→T L203L (CTG→CTA yfaY ← IPR008135 CinA family protein YfaY
RA 2,397,444 G→A Q908* (CAA→TAA)  nuoG ← NADH:quinone oxidoreductase subunit G
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,515,819 T→C T672A (ACG→GCG)  pdeA ← putative c‑di‑GMP phosphodiesterase PdeA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,603,942 C→T S32S (TCC→TCT hyfC → hydrogenase 4 component C
JC JC 2,666,074 IS1 (+) +9 bp coding (640‑648/1281 nt) csiE → stationary phase‑inducible protein CsiE
RA 2,686,812 G→C Q326H (CAG→CAC hmp → nitric oxide dioxygenase
RA 2,864,407 C→T L58L (CTG→TTG)  ygbM → putative 2‑oxo‑tetronate isomerase YgbM
RA 2,887,602 C→T R237R (AGG→AGA cysH ← phosphoadenosine phosphosulfate reductase
RA 2,899,684 C→T G197D (GGC→GAC)  ygcW ← putative deoxygluconate dehydrogenase
RA 3,162,858 C→T Q208Q (CAG→CAA plsC ← 1‑acylglycerol‑3‑phosphate O‑acyltransferase PlsC
RA 3,268,970 A→G T186A (ACA→GCA)  yhaC → uncharacterized protein YhaC
RA 3,273,707 C→T V401M (GTG→ATG)  garP ← galactarate/D‑glucarate transporter GarP
RA 3,398,259 G→A S40S (AGC→AGT yhdE ← nucleoside triphosphate pyrophosphatase YhdE
RA 3,411,854 G→A V68M (GTG→ATG)  yhdJ → DNA adenine methyltransferase
RA 3,429,941 C→T G28D (GGT→GAT)  yrdB ← DUF1488 domain‑containing protein YrdB
RA 3,431,615 C→T Q116Q (CAG→CAA yrdD ← putative DNA topoisomerase
RA 3,434,376 C→T L55L (CTG→TTG)  fmt → 10‑formyltetrahydrofolate:L‑methionyl‑tRNA(fMet) N‑formyltransferase
RA 3,436,817 C→T R100R (CGC→CGT trkA → NAD‑binding component of Trk potassium transporters
RA 3,438,489 C→T S54N (AGT→AAT)  arfA ← alternative ribosome‑rescue factor A
RA 3,438,497 C→T W51* (TGG→TGA arfA ← alternative ribosome‑rescue factor A
RA 3,441,328 C→T Q116Q (CAG→CAA rpsD ← 30S ribosomal subunit protein S4
RA 3,441,859 C→T K80K (AAG→AAA rpsK ← 30S ribosomal subunit protein S11
RA 3,442,095 C→T A2T (GCA→ACA)  rpsK ← 30S ribosomal subunit protein S11
RA 3,442,307 C→T T55T (ACG→ACA rpsM ← 30S ribosomal subunit protein S13
RA 3,444,895 C→T R112H (CGC→CAC)  rpsE ← 30S ribosomal subunit protein S5
RA 3,453,500 C→T intergenic (‑230/+8) rpsJ ← / ← gspB 30S ribosomal subunit protein S10/putative general secretion pathway protein B
RA 3,456,444 C→T A23V (GCT→GTT)  gspD → Type II secretion system protein GspD
RA 3,473,353 C→T E54E (GAG→GAA fusA ← elongation factor G
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,487,627 C→T R279R (CGC→CGT yhfK → putative transporter YhfK
RA 3,498,336 C→T A170V (GCC→GTC)  cysG → siroheme synthase
RA 3,498,660 C→T P278L (CCC→CTC)  cysG → siroheme synthase
RA 3,500,505 C→T A199V (GCG→GTG)  frlA → fructoselysine/psicoselysine transporter
RA 3,500,603 C→T L232L (CTG→TTG)  frlA → fructoselysine/psicoselysine transporter
RA 3,505,212 C→T S270N (AGC→AAC)  yhfS ← putative aminotransferase YhfS
RA 3,507,706 C→T intergenic (‑5/+6) yhfU ← / ← php DUF2620 domain‑containing protein YhfU/putative hydrolase
RA 3,508,356 C→T A79T (GCC→ACC)  php ← putative hydrolase
RA 3,513,332 C→T E103K (GAA→AAA)  trpS ← tryptophan‑‑tRNA ligase
RA 3,513,380 C→T E87K (GAA→AAA)  trpS ← tryptophan‑‑tRNA ligase
RA 3,519,188 C→T intergenic (‑124/+277) aroK ← / ← hofQ shikimate kinase 1/DNA utilization protein HofQ
RA 3,521,516 C→T G153R (GGA→AGA)  hofN ← DNA utilization protein HofN
RA 3,527,435 C→T L323F (CTT→TTT)  igaA → inner membrane protein IgaA
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,682,261 Δ34 bp coding (1154‑1187/1287 nt) dctA ← C4 dicarboxylate/orotate:H(+) symporter
JC JC 3,737,137 IS2 (+) +5 bp coding (37‑41/825 nt) bax ← putative glycoside hydrolase Bax
RA 3,894,178 G→A G100S (GGC→AGC)  yieE → putative phosphopantetheinyl transferase
RA 4,025,904 T→C V306A (GTG→GCG)  ubiD → 3‑octaprenyl‑4‑hydroxybenzoate decarboxylase
RA 4,069,443 C→T intergenic (‑167/+32) yihQ ← / ← yihR sulfoquinovosidase/putative sulfoquinovose mutarotase YihR
RA 4,072,562 C→T M30I (ATG→ATA yihT ← 6‑deoxy‑6‑sulfofructose‑1‑phosphate aldolase
RA 4,072,783 C→T R263R (CGG→CGA yihU ← 3‑sulfolactaldehyde reductase
JC JC 4,123,923 IS2 (–) +5 bp coding (530‑534/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
JC JC 4,135,180 IS2 (–) +5 bp coding (1346‑1350/2181 nt) katG → catalase/hydroperoxidase HPI
RA 4,213,597 G→A A122T (GCA→ACA)  yjaA → stress response protein
RA 4,213,776 G→A F116F (TTC→TTT yjaB ← peptidyl‑lysine N‑acetyltransferase YjaB
RA 4,214,904 G→A E209K (GAA→AAA)  metA → homoserine O‑succinyltransferase
RA 4,219,936 G→A Q447Q (CAG→CAA aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,233,540 A→G intergenic (‑307/‑218) lysC ← / → pgi aspartate kinase III/glucose‑6‑phosphate isomerase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,306,168 C→T G144S (GGC→AGC)  yjcS ← linear primary‑alkylsulfatase
RA 4,306,177 C→T G141S (GGC→AGC)  yjcS ← linear primary‑alkylsulfatase
RA 4,306,896 G→A P302S (CCA→TCA)  alsK ← D‑allose kinase
RA 4,307,202 G→A Q200* (CAG→TAG)  alsK ← D‑allose kinase
RA 4,308,804 G→A F222F (TTC→TTT alsC ← D‑allose ABC transporter membrane subunit
RA 4,309,387 G→A T28I (ACC→ATC)  alsC ← D‑allose ABC transporter membrane subunit
RA 4,313,713 G→A D122N (GAT→AAT)  rpiB → allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase B
RA 4,314,514 G→A P197S (CCG→TCG)  phnP ← 5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
RA 4,316,393 G→A R276C (CGC→TGC)  phnM ← RPnTP hydrolase
RA 4,316,417 G→A L268L (CTG→TTG)  phnM ← RPnTP hydrolase
RA 4,316,467 G→A P251L (CCC→CTC)  phnM ← RPnTP hydrolase
RA 4,316,569 G→A A217V (GCC→GTC)  phnM ← RPnTP hydrolase
RA 4,317,998 G→A intergenic (‑103/+8) phnL ← / ← phnK methylphosphonate degradation complex subunit PhnL/carbon‑phosphorus lyase subunit PhnK
RA 4,319,543 G→A R22C (CGC→TGC)  phnJ ← carbon‑phosphorus lyase core complex subunit PhnJ
RA 4,320,013 G→A R217R (CGC→CGT phnI ← carbon‑phosphorus lyase core complex subunit PhnI
RA 4,320,398 G→A A89V (GCG→GTG)  phnI ← carbon‑phosphorus lyase core complex subunit PhnI
RA 4,321,220 G→A P10S (CCC→TCC)  phnH ← carbon‑phosphorus lyase core complex subunit PhnH
RA 4,325,598 G→A C48C (TGC→TGT yjdN ← PF06983 family protein YjdN
RA 4,326,570 G→A G55G (GGC→GGT yjdM ← zinc ribbon domain‑containing protein YjdM
RA 4,330,772 G→A D91N (GAT→AAT)  proP → osmolyte:H(+) symporter ProP
RA 4,330,799 G→A A100T (GCT→ACT)  proP → osmolyte:H(+) symporter ProP
RA 4,330,931 G→A E144K (GAA→AAA)  proP → osmolyte:H(+) symporter ProP
RA 4,338,118 G→A intergenic (‑189/+136) adiY ← / ← adiA DNA‑binding transcriptional activator AdiY/arginine decarboxylase, degradative
RA 4,338,465 G→A P686L (CCA→CTA)  adiA ← arginine decarboxylase, degradative
RA 4,339,929 C→T G198D (GGT→GAT)  adiA ← arginine decarboxylase, degradative
RA 4,342,135 C→T A75A (GCC→GCT melA → alpha‑galactosidase
RA 4,342,451 G→A E181K (GAA→AAA)  melA → alpha‑galactosidase
RA 4,342,582 G→A P224P (CCG→CCA melA → alpha‑galactosidase
RA 4,342,595 G→A A229T (GCT→ACT)  melA → alpha‑galactosidase
RA 4,344,255 G→A R296H (CGC→CAC)  melB → melibiose:H(+)/Na(+)/Li(+) symporter
RA 4,345,751 G→A L526L (CTG→TTG)  fumB ← fumarase B
RA 4,346,714 G→A P205S (CCC→TCC)  fumB ← fumarase B
RA 4,347,037 G→A A97V (GCC→GTC)  fumB ← fumarase B
RA 4,351,790 G→A intergenic (‑128/‑53) dcuS ← / → yjdI sensor histidine kinase DcuS/PF06902 family protein YjdI
RA 4,369,682 G→A A244V (GCC→GTC)  yjeH ← L‑methionine/branched chain amino acid exporter
RA 4,380,354 C→T R55H (CGC→CAC)  frdB ← fumarate reductase iron‑sulfur protein
RA 4,381,240 C→T G360D (GGC→GAC)  frdA ← fumarate reductase flavoprotein subunit
RA 4,381,966 C→T G118D (GGC→GAC)  frdA ← fumarate reductase flavoprotein subunit
RA 4,385,905 C→T L68F (CTC→TTC)  yjeO → DUF2645 domain‑containing inner membrane protein YjeO
RA 4,386,061 C→T A1104T (GCG→ACG)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,387,120 C→T E751K (GAA→AAA)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,387,757 C→T T538T (ACG→ACA mscM ← miniconductance mechanosensitive channel MscM
RA 4,388,794 C→T A193T (GCG→ACG)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,392,516 G→A noncoding (45/76 nt) glyX → tRNA‑Gly
RA 4,397,180 C→T R372R (CGC→CGT amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,403,803 C→T T168T (ACC→ACT hflC → regulator of FtsH protease
RA 4,405,622 C→T D312D (GAC→GAT purA → adenylosuccinate synthetase
RA 4,408,175 C→T L508L (CTG→TTG)  rnr → RNase R
RA 4,408,700 C→T R683C (CGC→TGC)  rnr → RNase R
RA 4,411,260 C→T intergenic (+9/‑42) yjfJ → / → yjfK PspA family protein YjfJ/DUF2491 domain‑containing protein YjfK
RA 4,421,451 A→G K20R (AAG→AGG)  ulaB → L‑ascorbate specific PTS enzyme IIB component
RA 4,424,407 C→T intergenic (+21/+109) ulaF → / ← yjfY L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY
RA 4,425,291 C→T H58H (CAC→CAT rpsF → 30S ribosomal subunit protein S6
RA 4,429,997 C→T S45F (TCT→TTT)  cycA → D‑serine/alanine/glycine/:H(+)symporter
RA 4,430,193 C→T C110C (TGC→TGT cycA → D‑serine/alanine/glycine/:H(+)symporter
RA 4,430,615 C→T S251F (TCC→TTC)  cycA → D‑serine/alanine/glycine/:H(+)symporter
RA 4,434,299 C→T L63F (CTT→TTT)  ytfH → putative transcriptional regulator YtfH
RA 4,437,808 C→T I34I (ATC→ATT ytfI → protein YtfI
RA 4,440,203 C→T G338D (GGT→GAT)  paeA ← putative cadaverine/putrescine exporter
RA 4,441,026 C→T G64S (GGT→AGT)  paeA ← putative cadaverine/putrescine exporter
RA 4,499,513 C→T intergenic (+13/‑1747) insD6 → / → yjgZ IS2 insertion element protein InsB/uncharacterized protein YjgZ
RA 4,521,815 A→G V69A (GTT→GCT)  yjhF ← putative transporter YjhF
RA 4,540,522 C→T intergenic (‑1021/‑435) nanC ← / → fimB N‑acetylneuraminate outer membrane channel/Type 1 fimbriae regulatory protein FimB
RA 4,542,683 Δ1 bp intergenic (+50/‑432) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
RA 4,542,990 C→T intergenic (+357/‑125) fimE → / → fimA regulator for fimA/type 1 fimbriae major subunit
RA 4,545,140 C→T C15C (TGC→TGT fimD → type I fimbriae usher protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 164506 164643 138 2 [0] [1] 2 [hrpB] [hrpB]
* * ÷ NC_000913 807321 807360 40 2 [1] [1] 2 ybhC/ybhB outer membrane lipoprotein YbhC/putative kinase inhibitor
* * ÷ NC_000913 1168591 1168623 33 2 [1] [0] 2 comR DNA‑binding transcriptional repressor ComR
* * ÷ NC_000913 1196268 1211429 15162 2 [1] [1] 2 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1304999 1305061 63 2 [1] [1] 2 oppD murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppD
* * ÷ NC_000913 1311151 1311312 162 2 [1] [1] 3 tonB Ton complex subunit TonB
* * ÷ NC_000913 1395315 1395332 18 2 [1] [1] 3 ynaI small conductance mechanosensitive channel YnaI
* * ÷ NC_000913 1978495 1979270–1978504 10–776 10 [0] [0] 15 insB5–insA5 insB5,insA5
* * ÷ NC_000913 2271785 2271843 59 2 [1] [1] 2 pdeN putative c‑di‑GMP phosphodiesterase PdeN
* * ÷ NC_000913 2626138 2626176 39 2 [1] [1] 2 ppx exopolyphosphatase
* * ÷ NC_000913 3239607 3239663 57 2 [1] [1] 3 alx/sstT putative membrane‑bound redox modulator Alx/serine/threonine:Na(+) symporter
* * ÷ NC_000913 3423895–3424233 3424444–3424239 7–550 2 [0] [1] 2 rrlD 23S ribosomal RNA
* * ÷ NC_000913 3781054 3781090 37 2 [1] [1] 2 lldD/trmL L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 381260 =NA (NA)10 (0.700) 9/310 0.3 NA noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 382183 NA (NA)noncoding (924/1331 nt) IS2 repeat region
* ? NC_000913 382179 =NA (NA)9 (0.640) 7/306 0.4 NA noncoding (920/1331 nt) IS2 repeat region
?NC_000913 = 382590 NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 = 12994980 (0.000)15 (1.090) 14/302 0.1 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1397238NA (NA)10 (0.700) 8/310 0.4 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)14 (0.990) 13/310 0.1 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 2288919 =NA (NA)14 (0.990) 10/308 0.2 93.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 4536236 = 1 (0.070)intergenic (‑2183/‑361) topAI/ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
* ? NC_000913 = 33059102 (0.140)14 (0.990) 12/308 0.1 80.0% intergenic (‑93/+61) mtr/deaD tryptophan:H(+) symporter Mtr/ATP‑dependent RNA helicase DeaD
?NC_000913 3306184 = 5 (0.350)coding (1677/1890 nt) deaD ATP‑dependent RNA helicase DeaD