Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	671,691	C→T	D166N (GAT→AAT)	lptE ←	lipopolysaccharide assembly protein LptE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	671,691	0	C	T	100.0%	23.7 / NA	12	D166N (GAT→AAT)	lptE	lipopolysaccharide assembly protein LptE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/9);  total (3/9)

CGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTT  >  NC_000913/671464‑671906                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        cGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGc                                                                                                                                                                                                   <  1:163328/250‑1 (MQ=255)                            gAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGt                                                                                                                                                                       >  1:251957/1‑251 (MQ=255)                                                     tAACANNGGNNNGTNNCCAAGTAAAAGATACGCCGCGCGNAGCCCTTCATTGAGCTGCGCGCGGAGTTGNTNCNGGTANNACCNAATCATCAGTNACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTNGNCGACGTCTGTTCTTCGTCGGAACGAATATTCNCAGCACGGATGCTTGGCAGCTTACNAATCNNCTNtnnnnnnnnn                                                                                                                                                                           >  2:242466/1‑213 (MQ=255)                                                          gagGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTTCAGGCGTTTCCTGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGTCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGtt                                                                                                                                         <  1:242466/251‑1 (MQ=255)                                                                             aaGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTGACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTGGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTAc                                                                                                                                                                  <  1:227315/207‑1 (MQ=255)                                                                                                               gcgcgGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCTGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAgaacg                                                                                               <  1:50988/240‑1 (MQ=255)                                                                                                                            ccGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGGACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATc                                                                        <  1:176488/250‑1 (MQ=255)                                                                                                                                                                       cgTGCAGGCGTTGCCGGAGTAGCCGTTGTGGTCGACGTCTGGTCGTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCgn                            <  1:62702/251‑2 (MQ=255)                                                                                                                                                                          gCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGAGCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATc                                                             <  1:190022/215‑1 (MQ=255)                                                                                                                                                                              gCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGatc                     >  1:211453/1‑251 (MQ=255)                                                                                                                                                                                     cGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTGTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTGGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAAGCGGGTAGATAGCACGGCCGGGGATCAACACGGTc                                       <  1:117639/226‑1 (MQ=255)                                                                                                                                                                                                  gtgGTCGACGTCTGTTCTTCGTCGGAACGAATATTCGCAGCACGGATGCTTGGCAGCTTACGAATCAGATGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGttc  <  1:251972/250‑2 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                                        CGAATCCTTGTGCCGCAGCTACCTGACGAACAGCGTCCTGGCTTTCCTGCAATAACAGAGGATCGTTACCAAGTAAAAGATACGCCGCGCGCAGCCCTTCATTGAGCTGCGCGCGGAGTTGTTCCGGGTACAACCGAATCATCAGTTACCCAGCGTGGTGGAGACGCGTGCAGGCGTTGCCGGAGTATCCGTTGTGGTCGACGTCTGTTCTTCGTCGGAACGAATATCCGCAGCACGGATGCTTGGCAGCTTACGAATCAGCTGTTCGGCAGCACGGTCGTACATCTCTTTTACGATCATGTCTTGTTCGTTATCTTTCGCTAACGCCATTTGCGGGTTATCGAAGAACGAACGGAAGACTTTGGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTT  >  NC_000913/671464‑671906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑