Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	276,792	G→A	E26E (GAG→GAA)	mmuM →	homocysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	276,792	0	G	A	100.0%	24.2 / NA	10	E26E (GAG→GAA)	mmuM	homocysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

CAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAG  >  NC_000913/276684‑276975                                                                                                             |                                                                                                                                                                                        cAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCCGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCg                                                                                                                       >  1:258774/1‑175 (MQ=255) cAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTGATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGc                                             <  1:169810/249‑1 (MQ=255)        gAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTTCTGCTGGATGGCGCGATGGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGATCTTATCCGCGAATTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCcggc                                    <  1:208848/251‑1 (MQ=255)           aCGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCggg                                 >  1:181176/1‑251 (MQ=255)                        gCCAGAACATGTCGCAGAATAATCCGTTTCGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATTGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGt                                                                                                      <  1:178046/169‑1 (MQ=255)                                        gAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAACTGGAAGCGCGAGGGTGTNNCTTAGCNNa                                                                                                                                                            >  2:19906/1‑99 (MQ=255)                                        gAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCGGGATGGCGCGATGGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGTCTTCGCCGCGCGAGGTCt               <  1:19906/240‑1 (MQ=255)                                                 gTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCGGGATGGCGCGATGGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGAGCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCan  <  1:29858/244‑2 (MQ=255)                                                                                              cgcgATGGCGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATATTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCggg                                 >  1:209123/1‑168 (MQ=255)                                                                                                      cGACGGAACTGGAAGCGCGAGGGTGTAACTTAGCCGAAAGCCTGTGGTCCGCCACAGTGCTGGTAGAAAAACCGGAGCTTATCCGCGAAGTGCATCTTGATTCCTACCGGGCGGGGGCGCAATGCGCGATc                                                              >  1:243174/1‑131 (MQ=255)                                                                                                             |                                                                                                                                                                                        CAACCCCGAAACGCAAAACAGGAGCCAGAACATGTCGCAGAATAATCCGTTACGCGCTCTTCTTGATAAACAGGATATCCTGCTGCTGGATGGCGCGATGGCGACGGAGCTGGAAGCGCGAGGGTGTAACTTAGCCGACAGCCTGTGGTCAGCCAAAGTGCTGGTAGAAAACCCGGAGCTTATCCGCGAAGTGCATCTTGATTACTACCGGGCGGGGGCGCAATGCGCGATCACTGCCAGCTATCAGGCGACGCCGGCGGGCTTCGCCGCGCGAGGTCTGGATGAAGCGCAG  >  NC_000913/276684‑276975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑