Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,316,569	G→A	A217V (GCC→GTC)	phnM ←	RPnTP hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,316,569	0	G	A	100.0%	47.5 / NA	19	A217V (GCC→GTC)	phnM	RPnTP hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/8);  total (11/8)

CACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGCGACACCAGCGTCACCGGCTCGCGCTGCACCAGTTTTTCAAACAGCGGCAGCGTGGTGTGATGCGGC  >  NC_000913/4316357‑4316802                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          cACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTgcg                                                                                                                                                                                                     >  1:112522/1‑251 (MQ=255)                 gCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGCGGCTTCCGCAGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGgacggc                                                                                                                                                                                                                                       >  1:70471/1‑201 (MQ=255)                         ncGNGCCNCNNCNNACANTATTCGGCGCGCCCATCAGCANNTTCATGCCANGCTTGCGCGAGGCTTCCGCAGCTTCGAACGTGGTGAGAAATTCGGCGATCACGNTGCCAAGCTNNGGNGATTCNGNNNCNNNGGNGTGGGTGGCGTCATCGTGGNTGNNAAGCGCNATTTTGCGCGCGCGGCGCAGgacggc                                                                                                                                                                                                                                       <  2:70471/192‑1 (MQ=255)                                                    ngcCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGATGGTGAGATTCAGAAACGTGGGCGTGGGCGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTg                                                                                                                                                     >  1:62900/2‑248 (MQ=255)                                                      cccATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGCGAAATTCGGCGCTCACGCTGCCACGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGGCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAggg                                                                                                                                                 >  1:148198/1‑250 (MQ=255)                                                         aTCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGc                                                                                                                                                                                      <  1:251954/210‑1 (MQ=255)                                                                     atgccatgcTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGGAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTgcggc                                                                                                                                                                                                   <  1:143553/185‑1 (MQ=255)                                                                                   cgAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCACGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACt                                                                                                                   >  1:104116/1‑251 (MQ=255)                                                                                   cgAGGCGTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATAcn                                                                                                                   <  1:202195/251‑2 (MQ=255)                                                                                                   tCGAACGAGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCACCGTGGGCGAGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACCGGACGGCGATGGATTCGCGATTCGGCTGCGACAAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCa                                                                                                                                                                   >  1:46910/1‑187 (MQ=255)                                                                                                                naaaTTCGGCGCTCACGCTGCCAAGCTGGTGAGATTCAGCACAGTGGGCGTGGGCGGCGTCATCGTGGCTGGCCAGCGCAATTTTTCGCGCGCGGAACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGgcg                                                                                             >  1:66235/2‑244 (MQ=255)                                                                                                                      cGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCACCGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGc                                                                                                      <  1:17738/229‑1 (MQ=255)                                                                                                                                   gCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGc                                                                    <  1:269548/250‑1 (MQ=255)                                                                                                                                             cgatcgtCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTTGCCCGGCGAGTGGTCCATCAGCGACACCAGCgn                                                         <  1:95437/245‑2 (MQ=255)                                                                                                                                              gagaTTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGNCTGGCAAGCGNAATNNTTCGNGNGNGGCACAGGACGGCGATGGATTCGCGATTCNGCTGCGACCAGCGTGCGGCGAGCNNCANNNGCNnttctt                                                                                                                                                                               <  2:240733/132‑1 (MQ=255)                                                                                                                                              gagaTTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGAGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTcttctt                                                                                                                                                                               >  1:240733/1‑132 (MQ=255)                                                                                                                                                                          gTCATCGTGGCTGGCAAGCGAAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGCGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGAATTTGCCCTGATAGTATTCGCGATACATCTCGCGGTTGGAGAACTGACGCTGGCCCGGCGAGTGGACCATCAGCGACACCAGTGTCACCGGCTCGCGCAGCACCAGTTTTTCa                             >  1:216064/1‑250 (MQ=255)                                                                                                                                                                                  ggctggcAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGAGTGCGGCGAGCGCCAGtt                                                                                                                                                                                        >  1:181299/1‑87 (MQ=255)                                                                                                                                                                                              cAATTTTTCGCGCGCGGCACAGGACGGCGCTGGATTCGCGATTCGGCTGCGACCCGCGTGAGGCGCACGACAGTTGCTCTTCTTAGTAATGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATACTATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGCGACCCCAGCGTCACCGGCTCGCGCTGCACCAGTTTTTc                              >  1:307977/1‑229 (MQ=255)                                                                                                                                                                                              cAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTTATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGgcg                                                                                             >  1:105123/1‑166 (MQ=255)                                                                                                                                                                                                     tCGCGCGAGGCACAGGACGGCGATGGATTCGCGATTCGGCTTCGACCAGAGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCAGCAGTGAGGGAGTCTTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGCGACTCCAGCGTCACCGGCTCGCGCTGCACCAGTTTTTCAAACAGCGGCAGCGTGGTGTGATGAGGc  <  1:20598/250‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                                          CACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGGCGAACTGGCGCTGGCCCGGCGAGTGGTCCATCAGCGACACCAGCGTCACCGGCTCGCGCTGCACCAGTTTTTCAAACAGCGGCAGCGTGGTGTGATGCGGC  >  NC_000913/4316357‑4316802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑