Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	627,548	G→A	L493L (CTG→CTA)	entE →	2,3‑dihydroxybenzoate‑AMP ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	627,548	0	G	A	100.0%	19.4 / NA	11	L493L (CTG→CTA)	entE	2,3‑dihydroxybenzoate‑AMP ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

GAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAAC‑‑‑CTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCGCATCAGCCTGAAGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTGACTGGGCCTTTGAACCGC  >  NC_000913/627362‑627778                                                                                                                                                                                              |                                                                                                                                                                                                                                       gaAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGAAGAAGAGATCGACAAC‑‑‑CTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGGAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTACGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGc                                                                                                                                                                        >  1:50590/1‑251 (MQ=255)        cAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAAC‑‑‑CTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTACGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGAcggc                                                                                                                                                                 <  1:154959/251‑1 (MQ=255)           aTTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTACGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGAcggc                                                                                                                                                                 <  1:279309/251‑1 (MQ=255)                        gcgAGAAGATCGCTGCCGAAGAGATCGACAAC‑‑‑CTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTACGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCa                                                                                                                                                                               >  1:101797/1‑221 (MQ=255)                                                             nctgctgcGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGATGCTGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTACGTGAACAGGGGATTGCCGAATTTAAATTACCGGATCGCGTGGAGAGTGTGGCTTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTAAGTCAGtggctgg                                                                                                                        >  1:66795/2‑242 (MQ=255)                                                                                     ntACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTAGTGGTAAAAGAGACGCTGCGCGCGGTGACGGTGCGTCGTTTCCTACGTGAACCGGGTATTGCCGTATTTACATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAACACAATTACGTCAGTGGCTGGCGTCACGCGCATCAGCCTGAAGGAGAGAACACg                                                                                       >  1:28737/2‑251 (MQ=255)                                                                                             cACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTACGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTAAGTCAGTGGCTGGCGTCACGCGCATCAGCCTGAAGGAGAGAACACGATGGCTAt                                                                               >  1:188917/1‑251 (MQ=255)                                                                                                                 gAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGATCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTAAGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGCTTCACTTc                                                                                                                                                                           >  1:147877/1‑139 (MQ=255)                                                                                                                                                                  tGCGCGCGGTGCAGGTGCGTCGTGTCCGACGTGAACAGGGTATTGCCGAAGTGAAATTACCGGATCGCGTGGAGGGTGTGTATTCACTTCCACTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACg                                                                                                                                   <  1:290941/130‑1 (MQ=255)                                                                                                                                                                        cgGTGCAGGTGCGTCTTTTCCTACGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAATTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCTCATCAGCCGGAAGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACTCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTGACTGGGCCTTTGAAcc    <  1:243317/251‑1 (MQ=255)                                                                                                                                                                          gTGCAGGTGCGTCGTTTCCTACGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCGCATCAGCCTGAAGGAGAGAAAACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTGACTGGGCCTTTGAACCGc  >  1:154900/1‑251 (MQ=255)                                                                                                                                                                                              |                                                                                                                                                                                                                                       GAAAGATCAGATTAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAAC‑‑‑CTGCTGCTGCGCCACCCGGCGGTGATCTACGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGCGCTTATCTGGTGGTAAAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGTGGAGTGTGTGGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTACGTCAGTGGCTGGCGTCACGCGCATCAGCCTGAAGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTGACTGGGCCTTTGAACCGC  >  NC_000913/627362‑627778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑