Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,316,393	G→A	R276C (CGC→TGC)	phnM ←	RPnTP hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,316,393	0	G	A	100.0%	22.6 / NA	10	R276C (CGC→TGC)	phnM	RPnTP hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/3);  total (7/3)

GCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCG  >  NC_000913/4316169‑4316607                                                                                                                                                                                                                                 |                                                                                                                                                                                                                       gCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCaac                                                                                                                                                                                               >  1:70004/1‑248 (MQ=255)                                                gATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACgtggtg                                                                                                                                               <  1:265710/251‑1 (MQ=255)                                                            ccAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTTGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTGCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCgn                                                                                                                                                                                                            <  1:36783/178‑2 (MQ=255)                                                                              ncAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCTGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTgg                                                                                                                 >  1:36553/2‑251 (MQ=255)                                                                                          ggTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGAGGGGTAGTAGTTGGAAGCGAGGATATCCAGCAGGCCAAGCCGCGCCAGTTCACTGGCCGCCACCATGCCGGAGTGCGAGACGCCGCACACAATATTCGGCGCGCCCATCAAAACGCTCATGCCATGCTTGCGCGAGGCTTACGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCCCGc                                                                                                                            >  1:216020/1‑228 (MQ=255)                                                                                                    gTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAAcg                                                                                                  <  1:105994/244‑1 (MQ=255)                                                                                                           ncGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGt                                                                                                                                                                                           >  1:60996/2‑148 (MQ=255)                                                                                                                                               tagtagTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTcgcgcgcg                                                >  1:182699/1‑251 (MQ=255)                                                                                                                                                                                             cACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGACGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTgcg  >  1:112522/1‑251 (MQ=255)                                                                                                                                                                                                             gCCGGAGTGCGAGCCGCCGCACACAATATTCGGCGCGCCCATCAACACGTTCATGCCATGCTTGCGCGCGGCTTCCGCAGCTTCGAACGTGGTGAGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGgacggc                                    >  1:70471/1‑201 (MQ=255)                                                                                                                                                                                                                     ncGNGCCNCNNCNNACANTATTCGGCGCGCCCATCAGCANNTTCATGCCANGCTTGCGCGAGGCTTCCGCAGCTTCGAACGTGGTGAGAAATTCGGCGATCACGNTGCCAAGCTNNGGNGATTCNGNNNCNNNGGNGTGGGTGGCGTCATCGTGGNTGNNAAGCGCNATTTTGCGCGCGCGGCGCAGgacggc                                    <  2:70471/192‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                       GCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCTGCGGCAGCGTAAAGCGGTTGCTCTGGTCATCGGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCG  >  NC_000913/4316169‑4316607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑