Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,508,356	C→T	A79T (GCC→ACC)	php ←	putative hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,508,356	0	C	T	100.0%	71.0 / NA	26	A79T (GCC→ACC)	php	putative hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/11);  total (15/11)

ACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGG  >  NC_000913/3508110‑3508594                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ccgacgGATGCGTGGAGTTCGGGCGTCCGGTTGGGTTATGCGCCAGCGCAGCGGCAA‑AAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCAATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCATTTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCTGGAAAAACGCGTCCTGGTAATAACCGGTACAGGtcacca                                                                                                                                                                                                                                           <  1:326233/250‑1 (MQ=255)                    cGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTcgc                                                                                                                                                                                                                         >  1:75679/1‑251 (MQ=255)                        cGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGa                                                                                                                                                                                                                                     <  1:108634/235‑1 (MQ=255)                                                        aataaataCCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCGGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCa                                                                                                                                                                                                          <  1:10924/230‑1 (MQ=255)                                                                                 gTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGCTACCCTGTTCAATTTCCTCGACCCTCTCCTGCGCCAGTTCCTGCACGCTGCGGATCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATcac                                                                                                                                                            >  1:331810/1‑251 (MQ=255)                                                                                                               ncgATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTcg                                                                                                                                                                                                                          >  1:192321/2‑159 (MQ=255)                                                                                                                               tNNNNNCCGNGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGtctct                                                                                                                                                                                                                          <  2:192321/143‑2 (MQ=255)                                                                                                                                   cTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACCTGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGcc                                                                                                                                                >  1:104206/1‑213 (MQ=255)                                                                                                                                           cATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAattgcg                                                                                                                                                                                                 >  1:2955/1‑156 (MQ=255)                                                                                                                                           cATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCa                                                                                                                                                                      >  1:187100/1‑183 (MQ=255)                                                                                                                                                              ttCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGGCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAAGTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTGCGCACTCGCCGGGTCATCAGGTCGTTCATCTCCCGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACg                                                                                <  1:167758/250‑1 (MQ=255)                                                                                                                                                                              tGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTAATCCCCGTCTCGCGCATTACATCAAGCATAAattgc                                                                                                                                                                                                  <  1:155995/120‑1 (MQ=255)                                                                                                                                                                                 gcCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCAtc                                                                                                                                                                    >  1:215721/1‑147 (MQ=255)                                                                                                                                                                                  ccAGTTCCTGCACGCTGCGGTTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCAGCAGGTCGTTCAGCGCCTGGCAAATGAACGCAt                                                                                                       <  1:272356/207‑1 (MQ=255)                                                                                                                                                                                  ccAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGCAACACGCGTCCTGGTAATAACCGGTACAGGTAACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATca                                                                                                                                                             >  1:287229/1‑153 (MQ=255)                                                                                                                                                                                     gTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATAATGATCAAGGCGGCAGTCCACGTTGttt                                                                          >  1:25729/1‑233 (MQ=255)                                                                                                                                                                                                     ggTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTGCAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGGCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCGATGCGGCAGTCCACGGTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCAt                                        <  1:280732/251‑1 (MQ=255)                                                                                                                                                                                                     ggTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATACATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCTGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAACGCCGgagaga                                                           >  1:329976/1‑232 (MQ=255)                                                                                                                                                                                                         gccgcaTGTTCCGGGAAAANNGCGTCCGGGNAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCNTAAATTGCGNNTTNCGCCCNANNNANNNGTNGGTCATCGCAATCACATTANGCNNGCCCCGNGTCATCAGGTCGTTCATCTCCTGGCa                                                                                                                  <  2:77087/169‑1 (MQ=255)                                                                                                                                                                                                         gCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGAGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCa                                                                                                                  >  1:77087/1‑173 (MQ=255)                                                                                                                                                                                                          ccACATGTTCGGGGAAAAACGCGTCCNGGTAATNNCCNGTCCAGGTCACCACGTTGANCCNNNTNNNGNGCATTNCANNAATCATAAANTGCGCATTGCGCCCCATGTAACGGTTGGCCATCTCAATCACATTCCGCACGCCNCGGGTCATCNNGTCGTTCATCTCCTGGCAAATNAACNNANNCNGATNaan                                                                                             >  2:74549/1‑192 (MQ=255)                                                                                                                                                                                                           cacaTGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCg                                   >  1:283002/1‑250 (MQ=255)                                                                                                                                                                                                                              gcgTCCTGGTAATAACCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGACC‑GGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAggcggc                                                                                        >  1:259740/1‑177 (MQ=255)                                                                                                                                                                                                                                           aaCCGGTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCTCGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATTCTGATCAAGTCGGCATTCCACGTTGTTTTTAAATCCGGAGAGATCAATATTCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAggg  <  1:165367/251‑1 (MQ=255)                                                                                                                                                                                                                                               ggTACAGGTCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAAcc                        <  1:273475/225‑1 (MQ=255)                                                                                                                                                                                                                                                   cAGGTNNCCNNNTTNNTCCCCGTCTCGCGCATTACATCANGCATAAattgcgcattgcgc                                                                                                                                                                                         >  2:238930/1‑60 (MQ=255)                                                                                                                                                                                                                                                   cAGGTCACCACGTTGATCGCCGTCTCGCGCATTACATCAAGCATAAattgcgcattgcgc                                                                                                                                                                                         <  1:238930/60‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                               ACGACGTATGCGTGGAGATCGGGCGTCCGGTCTGGTTATGCGCCAGCGCAGCGGCAATAAATACCTTCTCTTCCAGCGGCGTAATCTTTCCTTCGCTGGTGCCGATCTCCGCGATGATCCCGGCTTTCAGCTCCGTGCCATCGATACCCTGTTCAATTTCATCGACCATCTCCTGCGCCAGTTCCTGCACGCTGCGGGTCGCCACATGTTCCGGGAAAAACGCGTCCTGGTAATAACCGGTACAGGCCACCACGTTGATCCCCGTCTCGCGCATTACATCAAGCATAAATTGCGCATTGCGCCCCATGTAACGGTTGGTCATCTCAATCACATTACGCACGCCCCGGGTCATCAGGTCGTTCATCTCCTGGCAAATGAACGCATACTGATCAAGGCGGCAGTCCACGTTGTTTTTAAAGCCGGAGAGATCAATATGCAGATGCTCATGGGCGAGGGTGTAACCCGTCGGATCAAAACTCATAGGG  >  NC_000913/3508110‑3508594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑