Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,095,666	G→A	G181D (GGC→GAC)	hisA →	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,095,666	0	G	A	100.0%	37.6 / NA	14	G181D (GGC→GAC)	hisA	1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

GGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTG  >  NC_000913/2095468‑2095897                                                                                                                                                                                                       |                                                                                                                                                                                                                                        ggTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCTGTCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGTCCGGAAACATGTGCTGTGTACCGATATCGCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGc                                                                                                                                                                                      <  1:242325/250‑1 (MQ=255)                                      gcTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGatgatg                                                                                                                                                >  1:250123/1‑251 (MQ=255)                                               ccGTATTGACGAGCCAGGCAACAAGCAGGTGGCAGTCAGCCGCTGGCAAGAGAACTCGGGCGTTTAACTGGAACAACTGGTGGATCCCTATCTCCCCGTCGGCCTGACACATGTGCTGTGTACCGCTATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGACGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTCTTGGCGAc                                                                                                                                                          >  1:317823/1‑232 (MQ=255)                                                 gTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTTTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAgtgtn                                                                                                                                                                                                           <  1:66517/181‑2 (MQ=255)                                                                              gCAGTCAGCGGCTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGACACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAgt                                                                                                                                                                                                              >  1:138933/1‑149 (MQ=255)                                                                                 gTCAGCGGCTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCcgg                                                                                                                                                                       >  1:27200/1‑185 (MQ=255)                                                                                        ncTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTgg                                                                                                                          >  1:262820/2‑223 (MQ=255)                                                                                           ggCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGTCATTTCAGTCCTCcggcgg                                                                                                                                                                    <  1:250236/178‑1 (MQ=255)                                                                                           ggCAAGAGAACTCGGGCGTTGCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGtgtg                                                                                                                                                                                                           <  1:282044/139‑1 (MQ=255)                                                                                                   aaCTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGTCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGTCATTTCAGGCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTgg                                                                                                                          <  1:186734/212‑1 (MQ=255)                                                                                                                                                     ccTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGACTCTAACGTATCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGGAAATTCACCGTGAAGGAGGCCATCGCATGCTGGCAAAACGCATAATccc                                 >  1:170168/1‑251 (MQ=255)                                                                                                                                                                 gNTGNGTACCNATATNTCGCGCGACGGCACGNTGGCAGACGCTAACGTCTCNNTNTATGANGAAgt                                                                                                                                                                                                              <  2:138933/66‑1 (MQ=255)                                                                                                                                                                                    ngcgACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATgg            >  1:65915/2‑241 (MQ=255)                                                                                                                                                                                    cgcgACGGCACGCTGGCAGACTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGgtggtg  <  1:51503/251‑1 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                        GGTTTGAACGCTTCGGTGCCGATGCCTTAGTGCTGGCGCTGGATGTCCGTATTGACGAGCAAGGCAACAAGCAGGTGGCAGTCAGCGGCTGGCAAGAGAACTCGGGCGTTTCACTGGAACAACTGGTGGAAACCTATCTGCCCGTCGGCCTGAAACATGTGCTGTGTACCGATATCTCGCGCGACGGCACGCTGGCAGGCTCTAACGTCTCTTTATATGAAGAAGTGTGCGCCAGATATCCGCAGGTGGCATTTCAGTCCTCCGGCGGTATTGGCGACATTGATGATGTGGCGGCCCTGCGTGGCACTGGTGTGCGCGGCGTAATAGTTGGTCGGGCATTACTGGAAGGTAAATTCACCGTGAAGGAGGCCATCGCATGCTGGCAAAACGCATAATCCCATGTCTCGACGTTCGTGATGGTCAGGTGGTG  >  NC_000913/2095468‑2095897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑