Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,317,998	G→A	intergenic (‑103/+8)	phnL ← / ← phnK	methylphosphonate degradation complex subunit PhnL/carbon‑phosphorus lyase subunit PhnK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,317,998	0	G	A	100.0%	28.9 / NA	11	intergenic (‑103/+8)	phnL/phnK	methylphosphonate degradation complex subunit PhnL/carbon‑phosphorus lyase subunit PhnK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

GAATGGCCGTGGAGCACCACGCATTCGCCCGCGTTGACGGTGAGCGAGGCGCGATTGAGGACGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCC  >  NC_000913/4317768‑4318222                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 gAATGGCCGTGGAGCACCACGCATTCGCCCGCGTTGACGGTGAGCGAGGCGCGATTGAGGACGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACACCGGCTCAATTCTGCaa                                                                                                                                                                                                               >  1:122939/1‑250 (MQ=255)             aGCACCACGCATTCGCCCGCGTTGACGGTGAGCGAGGCGCGATTGAGGACGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTTGCGCGTCTTATCCGACCGACGGGGAGCGCGTTTTTAGGCCGGATAAGGCGTTTACGCCGCATCCGACACCGGCTCAATTCTGCAAAACCGATGacaac                                                                                                                                                                                                  <  1:309378/251‑3 (MQ=39)                                                ggCGCGATTGAGGACGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCGACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACACCGGCGCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgatgataccgcgtgtgcgc                                                                                                                                                               <  1:180174/251‑14 (MQ=255)                                                  cgcgATTGAGGACGGGCAGGCGCTCGCCTTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCGCGTGGTTCGGGTGTCGGATGCGACGCTGACGCTTCTTACCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCTACACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCa                                                                                                                                                             <  1:230325/251‑1 (MQ=39)                                                   gcgATTGAGGACGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCTTGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGgatg                                                                                                                                                                              <  1:153671/233‑1 (MQ=255)                                                                         aCGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTCTCGGATGCGCCGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACACCGGc                                                                                                                                                                                                                           >  1:122928/1‑166 (MQ=255)                                                                                                                                                                                      cTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTAAGCCGCATCCGACACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGATGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCCTGGTTGAAAATACCCACCGCGAGGTTCAGCTCCACAACCAGGCcgc                         >  1:47684/1‑251 (MQ=255)                                                                                                                                                                                             gAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGACACCGGCTCAATTCTGCAAACCCGATGACACCAGCAGCTGTGTATACGGATGATGCGGCTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTtc                                                                                                                    >  1:213487/1‑153 (MQ=255)                                                                                                                                                                                                            ccGGATAAGGCGTTTACGCCGCATCCGACACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCggg                                                                                                                                                                       <  1:114371/87‑1 (MQ=255)                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGACACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGcc  <  1:273107/251‑1 (MQ=255)                                                                                                                                                                                                                             nccgcATCCGACACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATcac                                                         >  1:80255/2‑180 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                 GAATGGCCGTGGAGCACCACGCATTCGCCCGCGTTGACGGTGAGCGAGGCGCGATTGAGGACGGGCAGGCGCACGCCGTTTTGCTGGTGCAGGATGAAGGTTTTACTGACGTTTTGTACGTTAATCATTTTTCGCCTCGTGGTTCGGGTGTCGGATGCGACGCTGACGCGTCTTATCCGACCTACGGGGAGCGCATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCACCGGCTCAATTCTGCAAAACCGATGACACCAGCAGCTGTGTATACGGATGATGCGGGTCGTCGAGCACGCGGTCGGTTAACCCACTCTCCACCACTTGCCCCTGCTTCATCACCAGCAAACGGTCCGCCAGCAGGCGGGCGACGCCTAAATCATGGGTGACAATCACCACCGCGAGGTTCAGCTCCACCACCAGGCCGCGCAGCAGGTCGAGCAGGCGGGCC  >  NC_000913/4317768‑4318222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑