Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,734,541 C→T A36V (GCT→GTT)  mepH → peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,734,5410CT100.0% 20.5 / NA 8A36V (GCT→GTT) mepHpeptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase MepH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/0);  total (8/0)

ATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAA‑AAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGC  >  NC_000913/1734367‑1734783
                                                                                                                                                                              |                                                                                                                                                                                                                                                   
aTTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGAGGATCAACCGTATTTAGACCACCCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGTTACCACTCATATCACCAAAAAAGAAGATAAAAAGAAAAGCACGGCAAACACCAA‑AAAGACCCAGAAAACAGCGaa                                                                                                                                                                         >  1:170769/1‑250 (MQ=255)
                   ntAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGTTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAA‑AAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGt                                                                                                                                                      >  1:95708/2‑250 (MQ=255)
                      aaTCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGACGCGGATAAACCGTATATAGATCACGACCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCACATGCTTCAAAGCAAGCCAGGGAGAGTTCTGTTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAC‑AAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACg                                                                                                                                                   >  1:152877/1‑250 (MQ=255)
                                                                        agcgGATAAACCGTATTTCGATCACGCTCTGTGCTTTAATTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGTTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAA‑AAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTCCGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCt                                                                                                >  1:143469/2‑251 (MQ=255)
                                                                                                                                              ccATGCTTCAAAGAAAGCCAGGGAGAGTTCTGTTACCACTCATATCACCAAAAACGCAGATAACAAGCAAAGCACGGCAACCACCAA‑ACAGACCCAGAAAACAGCGAACAAAGCCACCCGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCTTTAAAAAATCTTCCATTCCCGCTTCTAAAAAc                                                                                          >  1:234390/1‑187 (MQ=255)
                                                                                                                                                           caagcCAGGGAGCGTTCTGTTACCACTCATATCACCAAAAAAGCAGATAAACAGAAAAGCACGCCAACC‑CCAACAAAGACCAAGACAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACAAAAAGCAAAACCGCTTCTTCCGTTAAACAATCTTCCATTACCGCTTCTAAAAACGACAAAACt                                                                                 >  1:178876/1‑183 (MQ=255)
                                                                                                                                                                 aGGGAGAGTTCTGTTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAAACACCAA‑AACGACCCAGAAAACAGCGAAAAAAGACGCCAGAAAAAGTACGACCAACAGCa                                                                                                                                         >  1:154508/1‑121 (MQ=255)
                                                                                                                                                                       ngTTCTGTTAACACTCCTATCACCACAACAGCAGATAAAAAGAAAAGCACGGCAACCACCAA‑AAAGACCCAGAAAACAGCGAAAAAAGCCGCCCGCAAAATTACGACTGAAAGCACAACGGCTCATTCCGTTAAAAAATCTTCCATTACCGCTTCTACAAACGCCAACACTCGAATCAAACACGCCGACAACAAAACGGCCTCAGCCAGCATTACAGAAAAGAGAACCAAACCAAAGGGATATCAATcca  >  1:28285/2‑248 (MQ=255)
                                                                                                                                                                              |                                                                                                                                                                                                                                                   
ATTAGAATCATCAAGTTTTGTAAATCAGACGCAGGCATGATAGACCTGCCTTTACAGAGGGACGCTCAGTGGCGCGGATAAACCGTATTTCGATCACGCTCTGTGCTTTACTTTTTACCACCCTGCCTTTAACGCCTATGGCCCATGCTTCAAAGCAAGCCAGGGAGAGTTCTGCTACCACTCATATCACCAAAAAAGCAGATAAAAAGAAAAGCACGGCAACCACCAA‑AAAGACCCAGAAAACAGCGAAAAAAGCCGCCAGTAAAAGTACGACCAAAAGCAAAACCGCTTCTTCCGTTAAAAAATCTTCCATTACCGCTTCTAAAAACGCCAAAACTCGCAGCAAACACGCCGTCAATAAAACGGCCTCAGCCAGCTTTACCGAAAAGTGTACCAAACGTAAGGGCTATAAATCGC  >  NC_000913/1734367‑1734783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑