Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,409,541	C→A	P11T (CCT→ACT)	dbpA →	ATP‑dependent RNA helicase DbpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,409,541	0	C	A	100.0%	29.7 / NA	12	P11T (CCT→ACT)	dbpA	ATP‑dependent RNA helicase DbpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/6);  total (6/6)

CTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAGATCTGCGAGGATACGCGCCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGCCTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTGCAGGCGAAAACCGGCAGCGGCAAAACGGCGGCTTTTGGCCTCGGCTTGTTACAGCAAATTGATGCGTCGCTATTTCAAACCCAGGCTTTAGTGCTGTGTCCTACGCGTGAACTGGCGGATC  >  NC_000913/1409387‑1409766                                                                                                                                                           |                                                                                                                                                                                                                                  cTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAGATCTGCGAGGATCCGCGCCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATg                                                                                                                                   >  1:147346/1‑251 (MQ=255)                tGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAGATCTGCGAGGATACGCGCCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTGCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGGTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTgn                                                                                                                          <  1:65567/245‑2 (MQ=255)                         caccacCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAGATCTGCGAGGATACGCGCCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTGCAGGCGAAAACCGGc                                                                                                            >  1:125393/1‑250 (MQ=255)                                    gaagaaCCGATTAAATTGTCGCATCGTGGGCGTAGATCTGCGAGGATACGCGCCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGc                                                                                                                                                                          <  1:323167/177‑1 (MQ=255)                                                                    tAGATCTGCGAGGATACGCGCCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTGCAgg                                                                                                                       <  1:49941/196‑1 (MQ=255)                                                                                    ngcgcCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTGCAGGCGAAAACCGGCAGCGGCAAAACGGCGGCTTTTGGCCTCGGc                                                                              >  1:204151/2‑221 (MQ=255)                                                                                       gcCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCGACCCTGAATGTGTTGACTCCCGCCCAACGCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGgccgc                                                                                                                                                                   <  1:175339/133‑1 (MQ=255)                                                                                                       ncGATGACCACGAGACTAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTAACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTGCAgg                                                                                                                       >  1:82245/2‑161 (MQ=255)                                                                                                         gATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCGTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTgn                                                                                                                          <  1:59495/156‑2 (MQ=255)                                                                                                              ccACGAGTATAGATTGTGACCGCTTTTTATACCCTGCATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCTGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCAGGAAAAGTTGTTCGCGTGCAGGCGAAAACCGGCAGCGGCAAAACGGCGGCTTTTGGCCTCGGCTTGTTACAGCAAATTGATGCGTCGCTATTTCAAACCCAGGCTTTAGAGCTGTGTcc                      >  1:278066/1‑251 (MQ=255)                                                                                                                    gaATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGACCCTTCATGCGTTGGGTTAtttca                                                                                                                                                                                         >  1:47698/1‑80 (MQ=255)                                                                                                                                  cGCTTTTTCTACCCTGAATGTTTTGACTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTGCAGGCGAAAACCGGCAGCGGCAAAACGGCGGCTTTTGGCCTCGGCTTGTTACAGCAAATTGATGCGTCGCTATTTCAAACCCAGGCTTTAGTGCTGTGTCCTACGCGTGAACTGGCGGATc  <  1:45734/251‑1 (MQ=255)                                                                                                                                                           |                                                                                                                                                                                                                                  CTCGCCCGCAACCCATGCCTGACCCACCACCCGATGAAGAACCGATTAAATTGTCGCATCGTGAGCGTAGATCTGCGAGGATACGCGCCTGCTAACTTTGCGTCGATGACCACGAGAATAGATTGTGACCGCTTTTTCTACCCTGAATGTTTTGCCTCCCGCCCAACTCACGAACCTTAATGAGTTGGGTTATTTAACCATGACGCCGGTGCAGGCCGCCGCGCTTCCGGCGATCCTTGCCGGAAAAGATGTTCGCGTGCAGGCGAAAACCGGCAGCGGCAAAACGGCGGCTTTTGGCCTCGGCTTGTTACAGCAAATTGATGCGTCGCTATTTCAAACCCAGGCTTTAGTGCTGTGTCCTACGCGTGAACTGGCGGATC  >  NC_000913/1409387‑1409766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑