Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,359,466	G→A	intergenic (‑125/+24)	ymjE ← / ← puuA	protein YmjE/glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,359,466	0	G	A	100.0%	13.9 / NA	7	intergenic (‑125/+24)	ymjE/puuA	protein YmjE/glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

CCTGAAATCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTGATAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTGATCATTCTCGATAAACTCACCCAGGGCATCGCTCTGGCGAATCGGAAAGGGTAAGCC  >  NC_000913/1359231‑1359672                                                                                                                                                                                                                                            |                                                                                                                                                                                                               ccTGAAATCAAACCTGTTTAGAAAGGGAACCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCg                                                                                                                                                                                                  >  1:86120/1‑250 (MQ=255)                    aGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCATGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTGCCTCGGCACAGTCTTAATCGTTAACGGTAGCTCTTTGCCGCCCGCGCTCCATAACGCGATATCCGGGAAATGGCTGAATTACCATCAAATGTCAGGGTTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGtttt                                                                                                                                                                              <  1:176820/251‑1 (MQ=255)                                                              ngggCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGCCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCa                                                                                                                                     >  1:196997/2‑250 (MQ=255)                                                                                                                                        tAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGTGTGTTTCAACATCCACTCAATTTCTGTTTCTGGGATGAGGCGCTTAAACTGCAACAGCGCATCATTTTTACAGGCGTGAGAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTg                                                           <  1:218920/250‑1 (MQ=255)                                                                                                                                                 aCGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCAGGTTTACAGGCGTGATAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTGATCATTCTCg                                                 <  1:300496/251‑1 (MQ=255)                                                                                                                                                                                                tGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTGATAGAAATGGCAGAAGCGTTCGCCTAACTAGCGGCGCAGGTGATCATTCTCGATAAACTCACCCAGGGCATCGCTCTGGCGAATCGGAAAGGGTAAGcc  >  1:181714/1‑251 (MQ=255)                                                                                                                                                                                                                               cTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTGATAGACATGGCAGAAGCGTTCGCCTAATTAGCGGCGCAGGTGATCATTCTCGATa                                              >  1:320123/1‑176 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                               CCTGAAATCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTGATAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTGATCATTCTCGATAAACTCACCCAGGGCATCGCTCTGGCGAATCGGAAAGGGTAAGCC  >  NC_000913/1359231‑1359672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑