Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,273,707 C→T V401M (GTG→ATG)  garP ← galactarate/D‑glucarate transporter GarP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,273,7070CT100.0% 58.6 / NA 22V401M (GTG→ATG) garPgalactarate/D‑glucarate transporter GarP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/10);  total (12/10)

GCTGACCAGCAACCAATTTGTACCTGTTTCGCAGCCAGTGCGGCTTTGAATTTATTCGGGAAAACATCGTTATTCATCGCTTATACCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGATGGTGGAAGCCAGCAACATTCCCAGCACAATCGGTAGC  >  NC_000913/3273481‑3273933
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  
gCTGACCAGCAACCAATTTGTACCTGTTTCGCAGCCAGTGCGGCTTTGAGTTTATTCGGGAAAACATCGTTATTCATCGCTTATACCTTTGCTTAATTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGACAATCATCAGTGGAGTGACAATGGAGGCAAc                                                                                                                                                                                                            >  1:215556/1‑251 (MQ=255)
     ccAGCAACCAATTTGTACCTGTTTCGCAGCCAGTGCGGCTTTGAATTTATTCGGGAAAACATCGTTATTCATCGCTTATACCTTTGCTTATTTCTGCAATTCCATACGTTTCATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGc                                                                                                                                                                                                       >  1:237916/1‑251 (MQ=255)
                               cAGCCAGTGCGGCTTTGAATTTATTCGGGAAACCATCGTTATTCATCGCTTATACCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGCCGTTAAAGAcgccgccgc                                                                                                                                                                               >  1:56480/1‑249 (MQ=255)
                               cAGCCAGTGCGGCTTTGAATTTATTCGGGAAAACATCGTTATTCATCGCTTATACCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGc                                                                                                                                                                                                               <  1:112424/217‑1 (MQ=255)
                                          gCTTTTAATTTATTCGGGAAAACATCGTTATTCATCGCTTATACCTTTGCTTATGTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCGGCATTGAAGGAGTGCATTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGAAAAGGGAGGCAGCATTGCCAAAGACGTTAAAGAcgccgccgc                                                                                                                                                                               <  1:137556/238‑1 (MQ=255)
                                                                                  ataCCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGTCCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGccagcccag                                                                                                                           <  1:144369/250‑1 (MQ=255)
                                                                                    aCCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAgcgc                                                                                                                        >  1:172896/1‑251 (MQ=255)
                                                                                                     cAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCa                                                                                                        >  1:74665/1‑250 (MQ=255)
                                                                                                                               cTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGAc                                                                                                                                                                                                                        <  1:191016/112‑1 (MQ=255)
                                                                                                                                                           cAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGTAGGCGTGCAGTTCACTTCCCAGGTAGCAAATCATAAGTGGAGTGACAATGGAGGCAACATTGACAAAGACGTTAAAGACGCCGCCGCAGAGGCAAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCAAGCGCACCAAATCCTTTGCCAAAGAAAGCAAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTAc                                                 >  1:187681/1‑251 (MQ=255)
                                                                                                                                                                     cATCCCACNANNNCNNGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCNTCAGTGGAGTGACAANGGNGGCCNCATNGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCgg                                                                                                                                    >  2:229414/1‑158 (MQ=255)
                                                                                                                                                                     cATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGTTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCgg                                                                                                                                    <  1:229414/158‑1 (MQ=255)
                                                                                                                                                                       tCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGAtgg                                     >  1:174137/1‑251 (MQ=255)
                                                                                                                                                                        cccACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGgtg                                                        <  1:108374/231‑1 (MQ=255)
                                                                                                                                                                          cACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTGTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGGGTAGTTACATAAGATGAtggtgg                                  <  1:272486/251‑1 (MQ=255)
                                                                                                                                                                                         tgcATTGAAGGAGTGCAGGTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAAAGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTGCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAAGCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGAtggt                                    <  1:132975/234‑1 (MQ=255)
                                                                                                                                                                                             ttGAAGGCGTGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGAAACATTGCCAAAGACGTTAAAGACCCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTAAGa                                                                                                                                             >  1:291119/1‑125 (MQ=255)
                                                                                                                                                                                                     ntGCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACCTTGCCAAAGACGTTAAAGACGCCGCCGCAGAAGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCg                                                                                                                                     >  1:67331/2‑125 (MQ=255)
                                                                                                                                                                                                       gCAGTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTGTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGATGGTGGAAGcn                             <  1:36697/227‑2 (MQ=255)
                                                                                                                                                                                                          gTTCACTTACCAGGTAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGATGGTGGAAGCCAGCAACATTCTCAGCACAATCGGTAGc  >  1:12269/1‑251 (MQ=255)
                                                                                                                                                                                                                        tAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGAc                                                                                                                                                                                                <  1:20138/47‑1 (MQ=255)
                                                                                                                                                                                                                        tAGCCAATCATCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGAc                                                                                                                                                                                                >  2:20138/1‑47 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                  
GCTGACCAGCAACCAATTTGTACCTGTTTCGCAGCCAGTGCGGCTTTGAATTTATTCGGGAAAACATCGTTATTCATCGCTTATACCTTTGCTTATTTCTGCAATTCCATACGTTTAATGTCGCCAACTACGAAGAGGTAGCAGACCATCGCCATCAGCGCTGAACATCCCACGAAAACCAGTGCTGCATTGAAGGAGTGCAGTTCACTTACCAGGTAGCCAATCACCAGTGGAGTGACAATGGAGGCAACATTGCCAAAGACGTTAAAGACGCCGCCGCAGAGGCCAACAATCTCTTTCGGCGCGGTGTCAGAAATCACCGGCCAGCCCAGCGCACCAAATCCTTTGCCAAAGAAAGCCAGCGCCATCAGCATGACCACCAGCGTGGTGTTGTTGGTGTAGTTACATAAGATGATGGTGGAAGCCAGCAACATTCCCAGCACAATCGGTAGC  >  NC_000913/3273481‑3273933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑