Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,098,971	C→T	D215N (GAT→AAT)	ugd ←	UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,098,971	0	C	T	100.0%	28.4 / NA	12	D215N (GAT→AAT)	ugd	UDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

CTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTGCCGAGATCAGGTTATTCGGCACAGACTGGTAGTTCGCCAGTAACTGC‑TTGGTATCTTTCGGCAGACAATAACCACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCG  >  NC_000913/2098729‑2099164                                                                                                                                                                                                                                                    |                                                                                                                                                                                                  cTTGCGTGACAAAATGGCATCGGCAATAAAATCATTACGCGTGCGGTTCGCCTCGACAATTGCCGAGATCAGGTTATTTGGCACAGACAGGTAGTTCGCCAGTAACTGC‑TTGGTATCTTTCGGCAGACAATAACAACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTCGGTCGAGACAAACGCCTACGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGt                                                                                                                                                                                           >  1:55793/1‑251 (MQ=255)                            aaaaTCTTTACGCGTGCGGTTAGCATCGACAATTGCCGAGATCAGGTTATTCGGCACAGACTGGTAGTTCGCCAGTAACTGC‑TTGGTATCTTTCGGCAGACAATAACCACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATCGCCagn                                                                                                                                                                 <  1:197464/250‑2 (MQ=255)                                     aCGCGGGCGGTTAGCATCGACAATGGCCGAGAGCAGGTTATTCGGCACAGACGGGTAGTTCGCCAGTAACTGCGTTGG‑ATCTTTCGGCAGACAATAACCACCATAACCAAACGACTGATTGTTGTAATGGTTGCCAATACGTGTGTCGAGACAAACGCCTTCGATTATTTGACGGTAATGCAGTCCTAAACTTGCTGGATAGCTATTCAGTGCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGttt                                                                                                                                                        <  1:307598/250‑1 (MQ=255)                                            cGGTTAGCATCGACAATTGCCGAGATCAGGTTATTCGGCACAGACTGGTAGTTCGCCAGTAACTGC‑TTGGTATCTTTCGGCAGACAATAACCACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCaaaa                                                                                                                                                  >  1:7069/1‑249 (MQ=255)                                                                                ggCACAGACTGGTAGTTCGCCAGTAACTGC‑TTGGTATCTTTCGGCAGACAATAACCACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACan                                                                                                            <  1:59875/251‑2 (MQ=255)                                                                                      naCTGGTAGTTCGCCAGTAACTGC‑TTGGTATCTTTCGGCAGACAATAACCACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATc                                                                                                                                                                       >  1:30493/2‑186 (MQ=255)                                                                                                             gctgggTATCTTTCGGCAGACAATAACCACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCGTCTATTATGTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCg                                                                                                                                        <  1:242183/191‑1 (MQ=255)                                                                                                                                       ccaccaTAACCAAACGACGGCTTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAACCGCATTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTACAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCt                                                                                           >  1:270046/1‑214 (MQ=255)                                                                                                                                                   aaCGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATAATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCACCACGCATCGCAAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTg                                           >  1:158559/1‑250 (MQ=255)                                                                                                                                                                tgtAATGGTTGCCAATTCGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGAt                              <  1:3976/250‑1 (MQ=255)                                                                                                                                                                tgtAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCGGCGCGTTCTGAACGCTCACCGAt                              <  1:104488/250‑1 (MQ=255)                                                                                                                                                                                                                            naCCTAAACTTTCTGCATAGCTATTCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCg  >  1:95460/2‑218 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                  CTTGCGTGACAAAATGGCATCGGCAATAAAATCTTTACGCGTGCGGTTAGCATCGACAATTGCCGAGATCAGGTTATTCGGCACAGACTGGTAGTTCGCCAGTAACTGC‑TTGGTATCTTTCGGCAGACAATAACCACCATAACCAAACGACGGATTGTTGTAATGGTTGCCAATACGTGGGTCGAGACAAACGCCTTCGATTATTTGACGGGAATTCAGACCTAAACTTTCTGCATAGCTATCCAGTTCGTTAAAGTACGCCACGCGCATCGCCAGGTAGGTGTTTGCAAAAAGTTTAATCGCTTCTGCTTCAGTGGAGTCGGTAAACAGCATCGGGATATTTTGCTTAATCGCGCCTTCCTGTAACAGAGCAGCGAAACGTTCTGCGCGTTCTGAACGCTCACCGATGACAATACGTGAAGGATGGAGATTATCG  >  NC_000913/2098729‑2099164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑