Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	4,135,180	IS2 (–) +5 bp	coding (1346‑1350/2181 nt)	katG →	catalase/hydroperoxidase HPI

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	4135180 =	1 (0.070)	17 (1.200)	14/310	0.1	94.3%	coding (1346/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 4135184	1 (0.070)	14 (0.990)	12/310	0.1	93.1%	coding (1350/2181 nt)	katG	catalase/hydroperoxidase HPI
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134956‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  <  NC_000913/4499511‑4499280                                                                                                                                                                                                                                                                                                                                                                                                                                                                               GGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTAGTTTCATCAACGATCTGCAGGCGTTAAACGAAGCCTTTGCCCGTGACTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATATGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCC                                                                                                                                                                                                                     >  1:229275/1‑250                    CGTTTTGCTCCTGAGTTAGAGAAGATCTCTCGTCGTTTCCTCACCGATCCGCAGGCGGTAAACGAAGCCTTTGCCCGTGCCTGGGTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGACGTGCCGAAAGAAGATCTGATCTGGCAAGATACGCTGCCTCAGCCGATCTACAACCCGCCCGAGCAGGAAATTTGGATTTGCCCCTATATTTCCATACATCTGTTATCACTTA                                                                                                                                                                                                  >  1:250121/1‑250                                GAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAG                                                                                                                                                                                      <  1:8908/250‑1                                                                                      nnnnnnGCNNNNNCCTNGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATT                                                                                                                                                                                                                                    <  2:196023/144‑1                                                                                                  GCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAAn                                                                                                                                                  <  1:78177/220‑2                                                                                                            AACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCTGAAGTGTCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGTATTTTTCCCTATATTTCCATGCAGCTGTTATCACTTAACCCATTACAATCCCGCTGCCGCAGATGTTCCCGTGTCGAGCGATAGCCCATCGCACTATGCGGATGCCATTCGTTATAATGCTCGAAC                                                                                                         <  1:158849/251‑1                                                                                                                                   CCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACACCCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGCTGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGC                                                                                  >  1:243124/1‑251                                                                                                                                                                GCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTC                                                                                                    >  1:19064/1‑204                                                                                                                                                                 CCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTGGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCANAT                                                                                                                                                                       >  2:192368/1‑136                                                                                                                                                                               GATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCAGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACACATTACAAGCCCGCTGCCGCAGATATTCACGTGGCGAGC                                                                                                                                                        >  1:139790/1‑137                                                                                                                                                                                           TCCGCTGCCGCATCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGAACCTATATTTCCCGACATATGTTATCACTTACCCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGTCGAGCGATAACCCAGAGCACTATGC                                                                                                                                    >  1:222973/1‑145                                                                                                                                                                                           TCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATTTGGATTTGCCCCTNTATNTCCANACNTCTGTTATCACTTAANCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATANNCNNNCNCACTATGC                                                                                                                                    <  2:222973/145‑1                                                                                                                                                                                            CCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACCTTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGACATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCAC                         >  1:127227/1‑251                                                                                                                                                                                                                   CCCGACCGAGCAGGACATTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAn  <  1:195022/251‑2                                                                                                                                                                                                                                                                                                                                                                                                                                                                               GGTGACCGACCTGACGCTGCGTTTTGATCCTGAGTTCGAGAAGATCTCTCGTCGTTTCCTCAACGATCCGCAGGCGTTCAACGAAGCCTTTGCCCGTGCCTGGTTCAAACTGACGCACAGGGATATGGGGCCGAAATCTCGCTACATCGGGCCGGAAGTGCCGAAAGAAGATCTGATCTGGCAAGATCCGCTGCCGCAGCCGATCTACAACCCGACCGAGCAGGACATT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4134956‑4135184 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTAC  <  NC_000913/4499511‑4499280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.