Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,363,025	C→T	L203L (CTG→CTA)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,363,025	0	C	T	100.0%	27.5 / NA	12	L203L (CTG→CTA)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

TCAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGC  >  NC_000913/2362817‑2363271                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       tcAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGGCAACACGTTTAACAGCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTGCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCg                                                                                                                                                                                                                <  1:211255/249‑1 (MQ=255)                                                       gTCCTTCANTNNNTNNGAAAATCACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACANTTTTTCCATCGCCTGNTGNTCGCNTGCNGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTGCGCCCGGCGGTAGTTGTAGAGTGTCCAGGCCTTGGGCCAGATCGCTTTccncccncc                                                                                                                                                                                                          >  2:234059/1‑194 (MQ=255)                                                       gTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTGCGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGAc                                                                                                                                                                                                          <  1:234059/201‑1 (MQ=255)                                                                         aaaTCACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAgg                                                                                                                                      <  1:230817/251‑1 (MQ=255)                                                                                gCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCATGATTTCNt                                                                                                                               <  1:254328/251‑1 (MQ=255)                                                                                                cGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATc                                                                                                                    <  1:269522/246‑1 (MQ=255)                                                                                                                         ccATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGTACGNCCannnnn                                                                                                                                                                                                   >  2:195804/1‑137 (MQ=255)                                                                                                                                                 gtccggtGAGGTTCAGTTCGATGATAGGCATTGAGGAGCGAGAGCCCATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTTCTCGACCATTACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGTCAACGATTAAGc                                                              <  1:230476/251‑1 (MQ=255)                                                                                                                                                          gTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATAGCTTTCCGAACGACCAAAAGTAGTAAAAAGCCGACAAACCGGCGGCTGCGGTAAAGAAAATCGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAAAGATTAAGCTGAACGGcc                                                     >  1:330899/1‑250 (MQ=255)                                                                                                                                                          gTTTCAGTTCGATGAGTGGCATTGAGGAGCGATAGCCCATTGTTCCGCCCGGCGGTAGTGGTAGAGTGTCCAGGCGTTGTGCCAGATCGCTTGCCGAACGACCAAAAGTAGTCAAAGGCAGACAAGCTGGCGGCTGGGGTAAATAAAAGCGCGCGCTCAGGCGTGGCAGGATTTCGTCCTCGACGATCTCCTGAAGTTCTAACGGTACTCCGGGAGAAAAGAACATCAGGCAACGATTAAGCTGCACGGc                                                      <  1:163499/250‑1 (MQ=255)                                                                                                                                                                                            ncccATTGTTACGCCCGGCGGTAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGGTATTGATa                    >  1:95669/2‑250 (MQ=255)                                                                                                                                                                                                          ccGGCGGTAATTGTAGAGGGTCCAGGNTTTGTGNNAGNTCGCTTTCCGAACGACCAANAGNNNTNNNANGCAGANACNNCGGCGGCTGNGGTAAAGAAAAACGCCCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACNTTAAATTTTNNCGGTACGCCGGGAGGAAAGAANATCNNGNNANGATNaan                                                               >  2:69679/1‑192 (MQ=255)                                                                                                                                                                                                             gcggtAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTggcaggc  >  1:91600/1‑251 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                       TCAACGTCAGGCTGAACTGGCGGTTTTGCAATTCGCGACTGATCTGCGCGGGCAGTCCTTCAGTGCCTTCGAAAATCACGCTCTGTCCGGCAACACGTTTAACATCCAGCCACAGTTTTTCCATCGCCTGTTGCTCGCTTGCCGGTCCGGTGAGTTTCAGTTCGATGATAGGCATTGAGGAGCGATAGCCCATTGTTACGCCCGGCGGCAGTTGTAGAGTGTCCAGGCTTTGTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGC  >  NC_000913/2362817‑2363271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑