Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,499,513	C→T	intergenic (+13/‑1747)	insD6 → / → yjgZ	IS2 insertion element protein InsB/uncharacterized protein YjgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,513	0	C	T	100.0%	26.8 / NA	12	intergenic (+13/‑1747)	insD6/yjgZ	IS2 insertion element protein InsB/uncharacterized protein YjgZ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

GAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCG  >  NC_000913/4499276‑4499699                                                                                                                                                                                                                                              |                                                                                                                                                                                           nagagTAACGGAATAGCAGAGAGCTTCGTGAAAACGATACAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCACAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATg                                                                                                                                                                               >  1:58150/2‑251 (MQ=16)                                      tAAAGCGTGACTACATCAGTATCATGCCCAGACCAGACGGGGTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACa                                                                                                                                           >  1:281424/1‑250 (MQ=255)                                                     tcaggatcatgacacaaacAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGaa                                                                                                                                                                             >  1:258248/19‑201 (MQ=11)                                                                                             gAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCAGAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGAGAACAGATGTCTGGAAATGTAGGGGCAAAGCCCCTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTGTCAGTACATGCGACTGTATTACAGGTAGCCATATATGGTTACCTGTGATCAGGAAGAATAAGtn                                                                                   <  1:64088/251‑2 (MQ=18)                                                                                                        agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGtttn                                                                        <  1:79107/251‑2 (MQ=255)                                                                                                                  gCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTg                                                              <  1:135600/251‑1 (MQ=255)                                                                                                                          aCGAATGGCATCCGCATAGTGCGCTGGGGTATCGCTCGCCACGGGAATATCTGCGGCAGCTGGCTTGTAATGGGTTAAGTGATATCAGATGTCTGGAAATATAGGGGCAAATTCACTTTTTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGc                                                                                                                                        <  1:299455/169‑1 (MQ=18)                                                                                                                                                        aTCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAgg                         <  1:286451/250‑1 (MQ=255)                                                                                                                                                                            cTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAg                                                                                                                                         >  1:323557/1‑118 (MQ=255)                                                                                                                                                                              gCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCg  <  1:162374/251‑1 (MQ=255)                                                                                                                                                                                                              ananNTNNCTGNAAATATAGGGGCAAATCCACTNNGTGCTGATANGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGNCTGCCATATNNGANTACCTNTNNNCNNNAANAATAAGTGGCTGAGGTTTCNAANNGTCTGCNGGTATATTCCTCTCTCCCTTAAAAAc                                  <  2:71578/187‑1 (MQ=255)                                                                                                                                                                                                              aCAGATGTCTGGAAATATAGGGGCAAATCCACTTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGACGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAAc                                  >  1:71578/1‑187 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                           GAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCG  >  NC_000913/4499276‑4499699

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑