Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	778,850	C→T	L371L (CTG→TTG)	tolB →	Tol‑Pal system periplasmic protein TolB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	778,850	0	C	T	100.0%	24.9 / NA	11	L371L (CTG→TTG)	tolB	Tol‑Pal system periplasmic protein TolB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/7);  total (4/7)

CCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGC  >  NC_000913/778636‑778964                                                                                                                                                                                                                       |                                                                                                                   ccAGAACCTGGCATTTACTTCTGACCAGTCCGGTCGTCCGCAGGTTTATAAAGTTAATAACAACGGCGGTGCTCCACAACGTATTCCCTGGGAAGGTTCGCAGAACCAGGACGCTGATGTCAGCAGCGACGGTAAATTTAGTGTAATCGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACTGGAGGCGTACAACTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTgg                                                                                <  1:270734/251‑1 (MQ=255)           gCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACgn                                                                      <  1:65630/251‑2 (MQ=255)                           nggCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTAGTAACTGGGAAGGTTTGCAGAACAAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACCTTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTTTGTCGTAAACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTAc                                                      >  1:29265/2‑251 (MQ=255)                                           ggTTTATAAAGTGAATATCAACGGCGNTGCGCCNNAANGTATTACCTGGGAAGGTTCNCANNNCNNNGNTGCGGNTGNNAGAAGAGACNGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAANACGATCTGGNNACGGGAGGCGTACAAGTTTTGNCGTNNANNTNCCTNGa                                                                                                 >  2:76559/1‑192 (MQ=255)                                                   aaGTGAATATCAACGGCGGTGCGCCACAACGCATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGGAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCGGGCAACGGGAGGCGTACAAGTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTCCAGCTCTTCTCAGGGGATGGGATcc                              <  1:17547/251‑1 (MQ=255)                                                                 ggcggTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGt                <  1:191007/251‑1 (MQ=255)                                                                            acaACGTATTACCTGGGAAGGGTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCAGTATGGTAAGCTACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATg     <  1:150034/251‑1 (MQ=255)                                                                               aCGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGc  <  1:76559/251‑1 (MQ=255)                                                                                                                          agcagcGACGGTAAATTTATGGTAATGGTCAGCTCCACTGGTGGGCAGCCGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTc                                                                                   >  1:125433/1‑127 (MQ=255)                                                                                                                                                                  gtggGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTTTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGt                            >  1:139189/1‑142 (MQ=255)                                                                                                                                                                                      nanCANGATCTNGCAANGGGAGGCGTACAAGTNTTGTCGTCCACGTTCCTGGNNGNAACGCNAAggc                                                                                   <  2:125433/66‑3 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                   CCAGAACCTGGCATTTACTTCTGACCAGGCCGGTCGTCCGCAGGTTTATAAAGTGAATATCAACGGCGGTGCGCCACAACGTATTACCTGGGAAGGTTCGCAGAACCAGGATGCGGATGTCAGCAGCGACGGTAAATTTATGGTAATGGTCAGCTCCAATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGC  >  NC_000913/778636‑778964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑