Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,442,307	C→T	T55T (ACG→ACA)	rpsM ←	30S ribosomal subunit protein S13

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,442,307	0	C	T	100.0%	79.1 / NA	27	T55T (ACG→ACA)	rpsM	30S ribosomal subunit protein S13
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

ACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTACGTGCGTTGGTCTTGGTACGCTGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGCGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGC  >  NC_000913/3442072‑3442544                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              aCGTGCACGAATTGGTGCCTTTGTCATTATTCAATCAACCCGATTATTTCTTGCTCGGTTTGCGCGGACCCTTACGGGTACGTGCGTTGGTCTTGGTACGCTGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGACGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCt                                                                                                                                                                                                                                >  1:263868/1‑251 (MQ=255)                                                              gcgaggACCCTGACGGGTACGGGGGTTGGTCGTGGTACGCTGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGGAGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCGACAACGACAAATTTGGCAACTTCGACACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAg                                                                                                                                                                   <  1:23016/247‑1 (MQ=255)                                                                                 cGTGCGTTGGTCTTGGTACGCTGACCNCGAACCNNGANACCACGACGATGACGCAAANCGNNNTNNNANCCAAGNTCNNTCAGGCGCTNGATGCTCATGCTGATTTAACGGCGCAGATCACCTTCAACGACAAATTTGGCAANTTCGTCACGNNGTGTGTc                                                                                                                                                                                                                                          >  2:77316/1‑161 (MQ=255)                                                                                 cGTGCGTTGGTCTTGGTACGCTGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTc                                                                                                                                                                                                                                          <  1:77316/161‑1 (MQ=255)                                                                                      gTTGGTCTTGGTACGCTGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCCTCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCTGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTACCATc                                                                                                                                                                                                      >  1:315980/1‑192 (MQ=255)                                                                                            cTTGGTACGCGGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGGTCCATCAGGCTCTTGATTCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTGGGCAACTTCGTCACGCAGTTTGTCGATTTGTCCGTGAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGaa                                                                                                                                                                 <  1:171590/223‑1 (MQ=255)                                                                                                gTACGCTGAACGCGAAACGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTa                                                                                                                                  >  1:249067/1‑250 (MQ=255)                                                                                                   ngCTGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTg                                                                                                                                                                                                                                    >  1:204375/2‑149 (MQ=255)                                                                                                        aCNNNNNACCNGGAGTCGACGCCGAGGACGCCGACCTCGATAGCAACCAACTCCCATCAGTCGCTTGATGCTCATGCTGACTTCACGGAGCAGATCACCATCAACGTCACAGTTGGCAAATTCGTCACGCAGTGTGTCGATTTg                                                                                                                                                                                                                                    <  2:204375/144‑1 (MQ=255)                                                                                                                         cACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAAGTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCGCTGATCTTAACATCTGCAGCGATACCCGCGGCAGCCAGGATGGCTTTAGAACg                                                                                                                                                               <  1:121000/196‑1 (MQ=255)                                                                                                                                       aaaCCGCGATAGCAACCAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGcc                                                                                          <  1:116183/251‑1 (MQ=255)                                                                                                                                                      ccAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCAc                                                                           >  1:22615/1‑251 (MQ=255)                                                                                                                                                       cAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAAAGACCAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCAATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACt                                                                          >  1:305674/1‑251 (MQ=255)                                                                                                                                                                     cgcTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGacgt                                                                                                                                    >  1:72716/1‑176 (MQ=255)                                                                                                                                                                     cgcNTGATGCTCATGCTGATTTCACNNCGCAGATCANCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCANACAGCTCACNNATNTTAACNTNNNCNNNGANACCCGCTGCAGCCAGGATGNCTNNAGAACGNGTCTTGCCGACGCCATAAATCGAAGt                                                                                                                                    <  2:72716/179‑1 (MQ=255)                                                                                                                                                                             gCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGCTCTTAACATCTTCAGCGATACCCGCTg                                                                                                                                                                                     >  1:126058/1‑122 (MQ=255)                                                                                                                                                                                   gCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCTTCACGCAGTGTGTCGCTTTGTGCTTCAGACCGCTCACTGCTCTTAACATCTTCAGCGATACCTGCTGCAGCCAGGATGGCTTTAGGACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCCGGAATGTTACTGCCTGCTATActg                                                                                >  1:49324/1‑215 (MQ=255)                                                                                                                                                                                             cGGCGCAGNTNNNCNNCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTNCTTCAGACAGCTCACNGANCTTANCATNTTCAGCGATACCCGCTGc                                                                                                                                                                                    >  2:227680/1‑107 (MQ=255)                                                                                                                                                                                             cGGCGCAGATCACCTTCAACGACAAATTGGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGc                                                                                                                                                                                    <  1:227680/107‑1 (MQ=255)                                                                                                                                                                                                  cAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGc                               >  1:149777/1‑251 (MQ=255)                                                                                                                                                                                                   aGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGcc                              <  1:16208/251‑1 (MQ=255)                                                                                                                                                                                                             cAACGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATTCCTGCTATACGGGCCACTATGCACTCCTACTATGTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAg                     <  1:188903/250‑1 (MQ=255)                                                                                                                                                                                                                cGACAAATTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGAt                  >  1:22459/1‑250 (MQ=255)                                                                                                                                                                                                                     aaTTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCtn                                                                                                                                                                                     <  1:202244/82‑2 (MQ=255)                                                                                                                                                                                                                     aaTTTGGCAACTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTg                                                                                                                                                                                     >  2:202244/1‑82 (MQ=255)                                                                                                                                                                                                                            catcTTCGTCACGCAGTGTGTCGAGTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGc                                                                                                                                                                                                <  1:181517/61‑1 (MQ=255)                                                                                                                                                                                                                               cTTCGTCACGCAGTGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGGTAATGCCTGCAATACGGGCCACTAAGCACTACTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAACTGGAAACGc  >  1:297533/1‑251 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                              ACGTGCACGAATTGGTGCCTTTGCCATTATTCAATCACCCCGATTATTTCTTGATCGGTTTGCGCGGACCCTTACGGGTACGTGCGTTGGTCTTGGTACGCTGACCGCGAACCGGGAGACCACGACGATGACGCAAACCGCGATAGCAACCAAGATCCATCAGGCGCTTGATGCTCATGCTGATTTCACGGCGCAGATCACCTTCAACGACAAATTTGGCAACTTCGTCACGCAGCGTGTCGATTTGTCCTTCAGACAGCTCACTGATCTTAACATCTTCAGCGATACCCGCTGCAGCCAGGATGGCTTTAGAACGGGTCTTGCCGACGCCATAAATCGAAGTTAATGCGATTACGGCATGCTTATGATCAGGAATGTTAATGCCTGCTATACGGGCCACTATGCACTCCTACTATTTAATATGTACGTTCCATGCTGAAAAGCCCGTTTTCAGGATACTCAAATGGAAACGC  >  NC_000913/3442072‑3442544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑