Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,708,108	A→G	Y397C (TAC→TGC)	rsxC →	SoxR [2Fe‑2S] reducing system protein RsxC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,108	0	A	G	100.0%	35.0 / NA	12	Y397C (TAC→TGC)	rsxC	SoxR [2Fe‑2S] reducing system protein RsxC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/5);  total (7/5)

GCCCGCTAATGGGCTTTACCTTGCCATGGCTGGATGTCCCGGTCGTAAAGATTACCAACTGTCTGTTGGCTCCCTCTGCCAATGAACTTGGCGAACCACAGGAAGAACAAAGCTGCATCCGGTGTAGCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTG  >  NC_000913/1707934‑1708319                                                                                                                                                                               |                                                                                                                                                                                                                    gCCCGCTAATGGGCTTTACCTTGCCATGGCTGGATGTCCCGGTCTTAAAGATTACCAACTGTCTGTTGGCTCCCTCTGCCAATGAACTGGGCGAACCACAGGAAGAACAAAGCTGCATCCGGTGTAGCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACTCTATAAAGCTACCACGc                                                                                                                                                                            <  1:249033/216‑1 (MQ=255)                                            cGTAAAGATTACCAACTGTCTGTTGGCTCCCTCTGCCAATGAACTTGGCGAACCACAGGAAGAACAAAGCTGCATCCGGTGTAGCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTcc                                                                                              >  1:186809/1‑251 (MQ=255)                                                                 gtTGGCTCCCTCTGCCAATGAACTTGGCGAACCACAGGAAGAACGAAGCTGCATCCGGTGTAGCGCCTGTGCGGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTGGTGCTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACGACGGATAACATTGCTGATTGCATTGAAGATGGGGCTTGCGCTTGTGTTTACCCGAGCAat                                                                                                                    <  1:116324/208‑1 (MQ=255)                                                                    ggCTCCCTCTGCCAATGACCTTGGCGAACCACAGGAAGCACAACGCTGCATCCGGTGTGGCGCCTGTGCTGACGCATGCCCTGAGGATCTTTTGCAGCACCAGTTGTGCTGGTTCAGCAACGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGATTGTGGGGCTTGCGCGTGGGTTTGCCCGAGCCATATTCCCCTGGTACAATATTTCCGTCCGGAAAAAg                                                                                  >  1:292184/1‑239 (MQ=255)                                                                                                                    cATCCGGTGTAGCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACACGATAACGCTACCCCGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCg                      >  1:154168/1‑251 (MQ=255)                                                                                                                       ccGGTGTAGCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATAGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGa                     >  1:129305/1‑249 (MQ=255)                                                                                                                                       gCTGACGCCGGCCCTGCGGATCTGTTGCCGCAACAGTTGTGCGGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCATGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGTATAAGTCAATGCGCGTTTCGAAGCGCGCCAGGCTCGTc    <  1:270965/250‑1 (MQ=255)                                                                                                                                        cTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTg  >  1:47295/1‑251 (MQ=255)                                                                                                                                          gACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACACGATaaan                                                                                                                                                                                     <  1:195553/70‑2 (MQ=255)                                                                                                                                          gACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACACGATAAAg                                                                                                                                                                                     >  2:195553/1‑70 (MQ=255)                                                                                                                                                 gccctgcGGATATTTTGCCGCAACAGTTGTGATGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTc                       >  1:104899/1‑221 (MQ=255)                                                                                                                                                         gATCTGTTGCCGCAACAGTTGTGCTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCAAAACTTGGCTGATTGCATTGAATGTGGGGCTGTCGCGTGGGTTTGCCCGAGCAAGATTCCCCTTGTGCAATATTGCCGTTAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAga                                    <  1:224836/200‑2 (MQ=255)                                                                                                                                                                               |                                                                                                                                                                                                                    GCCCGCTAATGGGCTTTACCTTGCCATGGCTGGATGTCCCGGTCGTAAAGATTACCAACTGTCTGTTGGCTCCCTCTGCCAATGAACTTGGCGAACCACAGGAAGAACAAAGCTGCATCCGGTGTAGCGCCTGTGCTGACGCCTGCCCTGCGGATCTTTTGCCGCAACAGTTGTACTGGTTCAGCAAAGGTCAGCAACACGATAAAGCTACCACGCATAACATTGCTGATTGCATTGAATGTGGGGCTTGCGCGTGGGTTTGCCCGAGCAATATTCCCCTGGTGCAATATTTCCGTCAGGAAAAAGCTGAAATTGCGGCTATTCGTCAGGAAGAAAAGCGCGCCGCAGAAGCCAAAGCGCGTTTCGAAGCGCGCCAGGCTCGTCTG  >  NC_000913/1707934‑1708319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑