Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,386,061 C→T A1104T (GCG→ACG)  mscM ← miniconductance mechanosensitive channel MscM

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,386,0610CT90.5% 41.7 / NA 21A1104T (GCG→ACG) mscMminiconductance mechanosensitive channel MscM
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/13);  minor base G (0/2);  total (6/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGA  >  NC_000913/4385815‑4386285
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                
aacaacGTCTGCGATGTGTTGATGTATCTCGACGAAGACGATACCCGTGATGTTGGTGCGACAATCACGCTACCGCTCTTTTTTCCCTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGATTCTGTACCCAACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATTAGTTTTGTTTGTGAGCAGGATTGGTTCATCCGGCACACACACTTCCAAACTTCCCGTCTg                                                                                                                                                                                                                               >  1:286753/1‑250 (MQ=255)
          gCGATGTGTTGATGGAGCTCGAAGAAGACGACACCCGGGATGTCGGGTTGATAATTACGCTACCGCTCTTGTTCCCTTTCGGCTGGCTCGCGTTGTGGCGAAAACAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTGTTCTCTGCGCTGTCAGGTTTGTTTGTGAGCCGGATTGGTTCAGCCGGCACACACACTTACAAACTTCCCGTCTGACGACCTTTg                                                                                                                                                                                                                     <  1:138843/250‑1 (MQ=255)
           cGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTTGTTCATCCGGCACTCAAACTTACAAACTTCCCGTCTGACGACCTTTGcc                                                                                                                                                                                                                   <  1:258711/251‑1 (MQ=255)
                                                                        ccGCTCTTTTTCCCTTTTTTTTGGTTCGCTTTGTGGCGAAATAAACGCGGCTGGATTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGGCGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGGCGGGTTGGTTCATCTGGCACACACACTTATAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGACAGTGTTCTCCCCGTTTGTTTTCCGTTTCGGCTTTCCCGACGCCTCTGGa                                                                                                                                                        >  1:316855/1‑249 (MQ=255)
                                                                              tttttCCCTTTTTTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCgn                                                                                                                                                 <  1:200700/250‑2 (MQ=255)
                                                                                  tCCCTTTTTTCTGGTTCTCTTTGTGGCTAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCATTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCTCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAAt                                                                                                                                             <  1:309657/250‑1 (MQ=255)
                                                                                      ttttttgctGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCACTATCAGTTTTGTTTGAGAGCCGGATTGGTGCATCCGGCACACAAACTTACAAACGTCCCGTCTGACGTCCTTTGCCCGCAGACGTCAGCGTtc                                                                                                                                                                                                  <  1:137905/187‑1 (MQ=255)
                                                                                                             cGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGa                                                                                                                    >  1:243356/1‑249 (MQ=255)
                                                                                                               aaaaaaaCGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTGTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAagcc                                                                                                                <  1:305849/251‑1 (MQ=255)
                                                                                                                                                    gCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTATCGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTGCCAGACGCATCTGGAAGGTCGGGTATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGc                                                                           <  1:327612/251‑1 (MQ=255)
                                                                                                                                                            cGGCTACTGGCTGTGGCAGTTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCGTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACgg                                                                     <  1:72403/250‑1 (MQ=255)
                                                                                                                                                            cGGCTACTGGCTGTGGCAGTTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGGCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACgg                                                                     <  1:72409/250‑1 (MQ=255)
                                                                                                                                                                            cAGTTTTGTCTGCGCTATCAGGTTTGTTTGTGAGCCGGATTGGTGCATCCGTCACACAAATTTACAATCTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCGCCTCGTTTGTTTACCGGTGAGGCTTTCCAGGCGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTggg                                                                                                                          <  1:46288/180‑1 (MQ=255)
                                                                                                                                                                              gTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCTCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtg                                                                                                 <  1:318669/203‑1 (MQ=255)
                                                                                                                                                                                                   tttgtttaTGAGCCGGATTGGTGCATCCGGCACACAAACTTGCAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAggn                                                                                                                                                                          <  1:58521/110‑2 (MQ=255)
                                                                                                                                                                                                                        ttCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGAACCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATccn                      <  1:200725/237‑2 (MQ=255)
                                                                                                                                                                                                                        ttCATCCGGCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGNGGGNNNNNaannnnnnnnnn                                                                                                         >  2:200725/1‑144 (MQ=255)
                                                                                                                                                                                                                              cGGCACACAAACTTACAAACTTCCCGGCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCATCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGTCATACGGTGACCCATCTCAGCAGCGTAAATACGCATCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGa  <  1:151618/251‑1 (MQ=255)
                                                                                                                                                                                                                               ggCACACAAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCACGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGGGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGa  >  1:207548/1‑250 (MQ=255)
                                                                                                                                                                                                                                gCACACAAACTTACAAACTTCCCGTATGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGGGGCTTTCCAGACGCATCTGGAAGGGCGGGTATGGCATATAGATACCGTGGGCATGGAAGCCAGCCAGAGTCAGCGGGTGGAGCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGATCGAAAATCTGAATCCCCAGTTGCCGATCCACCAgaa  >  1:2284/1‑247 (MQ=255)
                                                                                                                                                                                                                                 cacacaAACTTACAAACTTCCCGTCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCGCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATcc         <  1:225887/241‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                
AACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAACGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACAAACTTCCCGCCTGACGACCTTTGCCCGCAGACGTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATCTCATGGCGTAGCGGCATACGGTGACCCATCTCAGCGGCGTAAATACGCAGCTCGAAAATCTGAATCCCCTGTTGCAGATCCACCAGGA  >  NC_000913/4385815‑4386285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: