Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	283,868	G→A	C223Y (TGT→TAT)	yagF →	D‑xylonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	283,868	0	G	A	100.0%	24.9 / NA	10	C223Y (TGT→TAT)	yagF	D‑xylonate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/7);  total (3/7)

GCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCG  >  NC_000913/283656‑284089                                                                                                                                                                                                                     |                                                                                                                                                                                                                              gcggcgGGCGGTGATCGGCGGAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACGATGCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGATCTCTCCCTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTcc                                                                                                                                                                                         <  1:120437/251‑1 (MQ=255)                         gACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGGGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCGCCCTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGGCAGTTCCTCGGCACTGCGGGCACCTCGCAgg                                                                                                                                                                 <  1:115325/251‑1 (MQ=255)                                    aaGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAg                                                                                                                                                      <  1:251687/251‑1 (MQ=255)                                     aGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCAGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCTAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAgg                                                                                                                                                     <  1:327764/251‑1 (MQ=255)                                                                                      aCTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAggn                                                                                                    <  1:36884/251‑2 (MQ=255)                                                                                                                        ngNNCGTNGGGGAAGACGCGGGCAAGGTGNNGACCATCGGNGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTANCGCGCCTGCNNCTNGCCGGNCNNNGNNNGTNAGTTCCTCGGCACGGCGGGNACNNCGCAGGNGGTCGCGGAGGCGCTGGGTCTGgcgc                                                                                                                                      <  2:77411/182‑1 (MQ=255)                                                                                                                        cgACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGGGGTCGCGGAGGCGCTGGGTCTGgcgc                                                                                                                                      >  1:77411/1‑183 (MQ=255)                                                                                                                                           gggCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCa                                                                                                                                                                   >  1:4414/1‑135 (MQ=255)                                                                                                                                                                                         tctcTGCAGGAGGCCGCCGAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCCCGCAGGGGGTCGCGGAGGCGCTGGGTCTGGCGCGGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGGGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCg  <  1:142304/247‑1 (MQ=255)                                                                                                                                                                                                         ncgAACTGGGCTATCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTACTCGGCCCGGCGGGCACCTCGCAGGAGGTAGCGGAGGCGCTGGGTCTGGCGCTGACGCACTCCGCGCTGGCGCCGTc                                                                                                             >  1:34384/2‑127 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                              GCGGCGGGCGGTGATCGGCGTAGCGACCTGCGATAAAGGGCTGCCCGCCACCATGATTGCGCTGGCCGCGATGCACGACCTGCCGACTATTCTGGTGCCGGGCGGGGCGACGCTGCCGCCGACCGTCGGGGAAGACGCGGGCAAGGTGCAGACCATCGGCGCGCGTTTCGCCAACCACGAACTCTCCCTGCAGGAGGCCGCCGAACTGGGCTGTCGCGCCTGCGCCTCGCCGGGCGGCGGGTGTCAGTTCCTCGGCACGGCGGGCACCTCGCAGGTGGTCGCGGAGGCGCTGGGTCTGGCGCTGCCGCACTCCGCGCTGGCGCCGTCCGGGCAGGCGGTGTGGCTGGAGATCGCCCGCCAGTCGGCGCGCGCGGTCAGCGAGCTGGATAGCCGCGGCATCACCACGCGGGATATCCTCTCCGATAAAGCCATCG  >  NC_000913/283656‑284089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑