Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	690,713	C→T	A57T (GCC→ACC)	lnt ←	apolipoprotein N‑acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	690,713	0	C	T	100.0%	33.0 / NA	13	A57T (GCC→ACC)	lnt	apolipoprotein N‑acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/4);  total (9/4)

GCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAA  >  NC_000913/690467‑690955                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   gcAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCTGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGTAGAc                                                                                                                                                                                                                                                <  1:18118/251‑1 (MQ=255)          cGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGAGGTTTTCGGCCACAGACGCGACAGCACGACAGCAAAAAGTCCGGTATACAGCGACAACTACGCTGCCAGAAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAACGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGTAGACTGGAGTgg                                                                                                                                                                                                                                        >  1:299584/1‑250 (MQ=255)                                                                   gCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCTGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCCGGcc                                                                                                                                                                                >  1:116123/1‑249 (MQ=255)                                                                                                                                                             naGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAAtt                                                                                             >  1:94211/2‑242 (MQ=255)                                                                                                                                                                        ccAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATACCCAGCCAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGa                                                                                        >  1:190276/1‑236 (MQ=255)                                                                                                                                                                             ggTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGTAGACTGGAGAGGANNGCGGttnn                                                                                                                                                                                                                             >  2:26991/1‑96 (MQ=255)                                                                                                                                                                             ggTCGCGATGCTGACATAGACACAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAAGAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATg                                                                           <  1:26991/244‑1 (MQ=255)                                                                                                                                                                              gTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCCAAGAGGCCAAATCCCCAGCACAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCACCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGc                                                                                                                                           >  1:133726/1‑179 (MQ=255)                                                                                                                                                                                      gCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGACTGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGt                                                           >  1:51829/1‑251 (MQ=255)                                                                                                                                                                                                                                 ccccAGCAAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCgn                <  1:95429/251‑2 (MQ=255)                                                                                                                                                                                                                                  cccAGCAAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCCGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCggg               >  1:298909/1‑251 (MQ=255)                                                                                                                                                                                                                                       cAAAAGCCAATAGCGGTAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTc                      >  1:229479/1‑239 (MQ=255)                                                                                                                                                                                                                                               aaTAGCGGTAGACTGGAGTGGACGGCGTTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGATAGAAGGCCAGCGTTCCGCAGGCGCCTAATAATAACTCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATTTAGTTATCGATCCGGGTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGaa  <  1:322337/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   GCAGAAACTCGGTCACTTGCCAGAGGGCAGGGGCGGCAATCGCTACGCGCAGCCAGGTGGTTTTCGGCCACAGACGCGACAGCACGCCAGCAAACAGTCCGGTATACAGCGACAAATACGCCGCCAGCAGCACCACCAGGAAGATGTTAACCGGGCCAGGCATTCCGCCAAAGGTCGCGATGCTGACATAGACCCAGTTAATACCGCTGCCAAAGAGGCCAAATCCCCAGCAAAAGCCAATAGCGGCAGACTGGAGTGGACGGCGGTTAAAGGTCAACGCCTGAAGCCCCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAA  >  NC_000913/690467‑690955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑