Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	779,360	C→T	N98N (AAC→AAT)	pal →	peptidoglycan‑associated outer membrane lipoprotein Pal

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	779,360	0	C	T	100.0%	27.8 / NA	12	N98N (AAC→AAT)	pal	peptidoglycan‑associated outer membrane lipoprotein Pal
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

GCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTC  >  NC_000913/779118‑779557                                                                                                                                                                                                                                                   |                                                                                                                                                                                                      gCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGATGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCTGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCtt                                                                                                                                                                                                >  1:324941/1‑250 (MQ=255)                    ccAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGACCGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCACATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGAACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAggg                                                                                                                                                                            >  1:314370/1‑250 (MQ=255)                                                                  cGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTg                                                                                                                              <  1:169995/251‑1 (MQ=255)                                                                   ggCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCACATCCAACAGCTGCAGCAGAACCACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTg                                                                                                                              >  1:187308/1‑250 (MQ=255)                                                                            aTGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTACAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATTCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCTTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTg                                                                                                                     <  1:174686/250‑1 (MQ=255)                                                                                                           cTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATacaaca                                                                                                                                          >  1:173526/1‑198 (MQ=255)                                                                                                              ccctggaattaaccagccggCAAATGCAACAGCTGCAGTAGAATAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCGGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGGCCGCCGGAATACAACATCGCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGc                                                                                  <  1:213859/232‑1 (MQ=255)                                                                                                                                                   gcagAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAAc                                                                                                                <  1:106567/184‑1 (MQ=255)                                                                                                                                                                              ggACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAGGTCACGCTGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGGCCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTggg                       <  1:318782/246‑1 (MQ=255)                                                                                                                                                                                              cGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCGGCGTAGCAGTCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGTGTAAAGGCGTTTCTGCAGACCAGAGTTCCATCGTTTCTTTCGGTATAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACtt  <  1:49534/251‑2 (MQ=255)                                                                                                                                                                                                                            naTGCANACTTCCTGCGTNGCAATCCGTCTTACAAAGTCACCGTAGANGGTCACGCGGa                                                                                                                                                                    <  2:48459/58‑1 (MQ=255)                                                                                                                                                                                                                            caTGCAAACTTCCTGCGTAGCAATCCGTCTTACAAAGTCACCGTAGAAGGTCACGCggc                                                                                                                                                                    >  1:48459/1‑58 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                      GCAATTGCGGCATGTTCTTCCAACAAGAACGCCAGCAATGACGGCAGCGAAGGCATGCTGGGTGCCGGCACTGGTATGGATGCGAACGGCGGCAACGGCAACATGTCTTCCGAAGAGCAGGCTCGTCTGCAAATGCAACAGCTGCAGCAGAACAACATCGTTTACTTCGATCTGGACAAGTACGATATCCGTTCTGACTTCGCTCAAATGCTGGATGCACATGCAAACTTCCTGCGTAGCAACCCGTCTTACAAAGTCACCGTAGAAGGTCACGCGGACGAACGTGGTACTCCGGAATACAACATCTCCCTGGGTGAACGTCGTGCGAACGCCGTTAAGATGTACCTGCAGGGTAAAGGCGTTTCTGCAGACCAGATCTCCATCGTTTCTTACGGTAAAGAAAAACCTGCAGTACTGGGTCATGACGAAGCGGCATACTC  >  NC_000913/779118‑779557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑