Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,431,615	C→T	Q116Q (CAG→CAA)	yrdD ←	putative DNA topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,431,615	0	C	T	100.0%	30.4 / NA	12	Q116Q (CAG→CAA)	yrdD	putative DNA topoisomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/5);  total (7/5)

CTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGCCCCTCCAGAACTTTGACGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTG  >  NC_000913/3431396‑3431856                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  ctTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGTTGGACCAGATGGCCCGTCCGACATTgggg                                                                                                                                                                                                                      <  1:89690/249‑1 (MQ=255)                                    nacncGGNTNNCNNCATNGTTTACTGGCACAAAAGGGTTNNACACCCTGCNCGGTTTTCTTTTCAATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTNTGGGTTGGANNTTNATGGCNANNNGNNNCTNCGGGTAGCGATCACAAGAGNGANNTGTTTTNCCATAACGGGAGCGGCGTTGGACCAg                                                                                                                                                                                                                                           <  2:70851/189‑1 (MQ=255)                                                                        gtTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACCCTCAGGGCATTCTCCAGCTTTGGGTTTGAAGTTAATGGCAAATTGACACTCCGGTTAGCGATCTCAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGTTGGACCAGATGGCCCGTCCGACATTGGGGGCATGCAATTGCTGTTTCGTCCGGTTTTTTGTTAAGGTCGGTATGTTCGCATTCAGGGTAGTTAATGCAAcc                                                                                                                                              >  1:21745/1‑250 (MQ=255)                                                                                                             cGGATAATGACACTCAGGGCATTCTCTAGCGATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGTTCACATGAGGGAAATGTTTTGGCATAATTGGAGCGGCGTTGGATCAGAGGGCTCGTCCGACATTGTGGGCATGTAATTGCTGTTTCGTCCGTTTTATCGAGGATTTCGGTATTTTCGCATTCATGGTAGTTAATGCTGCCGATAAACATACCAAAGCGTCCCTGGCGTAATACCAGAt                                                                                                        <  1:158954/251‑1 (MQ=255)                                                                                                                     gACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGTTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTAGCGTAATACCAGATTTGCGcc                                                                                                 >  1:185450/1‑250 (MQ=255)                                                                                                                             gggCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGTTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAgg                                                                                        <  1:250978/251‑1 (MQ=255)                                                                                                                                                gTTTGAAGGTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGCTTTGCCATAACGGGAGCGCCGTTGGACCAGATGGCCCGACCGCCATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGCTAAGTTAGGTATGGTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGCCCATcc                                                                        >  1:315807/1‑248 (MQ=255)                                                                                                                                                                        ncTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGTTGGAACAGATGGCCCGTCCGACATTGGGGGAATGTAATTGCt                                                                                                                                                                                                         >  1:58970/2‑95 (MQ=255)                                                                                                                                                                                                           ncATAACGGGAGCGGCGTTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGCCCCTCCAGAACTTTGACGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCacac               >  1:38536/2‑246 (MQ=255)                                                                                                                                                                                                           ccATAACGGGAGCGGCGTTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGGTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAATGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGcc                                                                             <  1:241265/184‑1 (MQ=255)                                                                                                                                                                                                           acATANNGGNNNCGNNGTTGGACCAGATGGCCCGTCCGANATTGGGGGCCTGTAATTGCTGTTTCGTCCNGNTNATCGNNAAGNTCGGTATGTTNGCATTCAGGGAAGTTAATGCAACCAATAAACATACCAAAGCGTACCTGNCNTAATACCAGATTTGCGCCAAATGCAGGGCAANCCTGcc                                                                             >  2:241265/2‑184 (MQ=255)                                                                                                                                                                                                                   ngAGCGGCGTTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCCCATGCAGGGCAAACCTGCCCCTCCAGAACTTTGACGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACtgtg  >  1:200448/2‑251 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  CTTTGCAGGTTATTATTCACGTTATTATTCCGCCGAAACCGGCTTTCCACATTGTTTACTGGCACAAAAGTGTTTTACACCCTGCGCGGTTTTCTTTTCGATGAGTAGCGGATAATGACACTCAGGGCATTCTCCAGCTATGGGTTTGAAGTTAATGGCAAATTGACACTCCGGGTAGCGATCACAAGAGTGAAATGTTTTGCCATAACGGGAGCGGCGCTGGACCAGATGGCCCGTCCGACATTGGGGGCATGTAATTGCTGTTTCGTCCGGTTTATCGATAAGTTCGGTATGTTCGCATTCAGGGTAGTTAATGCAACCAATAAACATACCAAAGCGTCCCTGGCGTAATACCAGATTTGCGCCACATGCAGGGCAAACCTGCCCCTCCAGAACTTTGACGATATGTCCATCCGCTGAAGATTTCAGAGGACGGACGTAGTCACACGCCGGATACTGTG  >  NC_000913/3431396‑3431856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑