Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,423	G→A	V219V (GTC→GTT)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,423	0	G	A	100.0%	25.7 / NA	12	V219V (GTC→GTT)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/5);  total (7/5)

ACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGG  >  NC_000913/2032217‑2032655                                                                                                                                                                                                               |                                                                                                                                                                                                                                         nccaACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGccct                                                                                                                                                                                              >  1:39579/2‑249 (MQ=255)                 catAAATTTGCCGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAACACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaaa                                                                                                                                                                               >  1:129414/1‑250 (MQ=255)                 catAAATTTGCAGTAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGCGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATAAACTCGACTaaaaa                                                                                                                                                                               >  1:312582/1‑250 (MQ=255)                          gcagcaGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGCTGCTGTATGAATCGGTTACTGATATATTTTCATGCATAGATTTCACAGACCATCTGAGATGGACGACAGGTTAACATAATTACAGCCATTTAGGGCAACGCCACCACCCAGCGGATGAAACCGCATATATCACCTTGGGATAAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGACCTTCGCCATAAACTCGCCTAAAAATTGCGCGTtg                                                                                                                                                                     >  1:242129/1‑251 (MQ=255)                                                            cccggttcgttATGAATCGGTTACTGATATATTTCCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACATCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCAGCTATCACCTTGTGATCAACACCTTCATTCTCCCCCTCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATTCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGc                                                                                                                                    <  1:219925/240‑1 (MQ=255)                                                               ggTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATgg                                                                                                                                <  1:258088/251‑1 (MQ=255)                                                                                                                                     ggTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAg                                                                                                                                                                                                       >  1:44569/1‑110 (MQ=255)                                                                                                                                                     gCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCGCTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCATCGCGTAGCGTTTATCATCAAGCGGGCGATGc                                                         <  1:260373/236‑1 (MQ=255)                                                                                                                                                                    gcgcTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAAGGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAgn                           <  1:34967/251‑2 (MQ=255)                                                                                                                                                                      gctaagccagcGGATGAAAAAGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCTCCATAAACTCGACTAAACAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGTTTTGCATGGTTTTCGGTAGCTTCAGCAACTTAGTCTGTAAATGATCCAGCGCGTAGCGTTTATCATCAAACGGGCGATGCTGTGCGAACGAATCTTCACCATCAAACAgcgc                         >  1:247716/5‑251 (MQ=255)                                                                                                                                                                                            cATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTNNGCTgcgnn                                                                                                                                                            >  2:12354/1‑96 (MQ=255)                                                                                                                                                                                              tCTATCCCCTTGTGATCACCGCCTTCATTCCCCCCCGCCAGTTCGGCACTGTGCTTCGCCATAAACTCGACTAAAAAATGCGCTTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTGGCATGTTTTGCGGTAGCTGCAGCAACTTAGTCTGGAAATGATCCAGCGCTAAGCGTTTATCACCAGGCGGGCGATGCTGTGCGAACGGATCTTCTCCACCAAACAGCGCCACGCCCAATGCCCCTGAAACgg  <  1:156932/250‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                         ACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAACGG  >  NC_000913/2032217‑2032655

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑