Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,500,505 C→T A199V (GCG→GTG)  frlA → fructoselysine/psicoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,500,5050CT91.7% 25.2 / ‑5.8 12A199V (GCG→GTG) frlAfructoselysine/psicoselysine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (8/3);  minor base G (1/0);  total (9/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATTTTGCCGCCCCTACCACCACTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTGCTGGCGGGGATCTCTGCCACCAGTTGGTCGTATACCGGCATGGCCTCTATCTGTTATATGACCGGCGAAATTAAAAACCCCGGAAAAACCATGCCACGAGCGCTGATTGGTTCCTGTCTGCTGGTTCTGGTGCTCTACACCCTGCTGGCGCTGGTGATTTCCGGCCTGATGCCCTTCGACAAACTCGCCAATTCTGAAACGCCGATTTCCGACGCCCTGACCTGGATCCCCGCACTCGGC  >  NC_000913/3500259‑3500740
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
gCTTTTTAACGCCTATCGATCCGTTGCTCGGTAACTTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATTTTGCCGCCCCTACCACCACTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTGCTGGTgggg                                                                                                                                                                                                                                         >  1:275651/1‑251 (MQ=255)
gCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGCCTTAATTGTCGCCTTTCTGTTGCTCCACCTGCGATCCGTTAAAGGCGGCGCAGCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCCCCATAGTCATTGCCCTTGGGATCTTCTGGTTTCAAGCGGAGAATTTTGCCGCCCCTACCACCCCTGCGATTGGCGCAACGCGCCGCTTTATGGCGCTGCTGGTgggg                                                                                                                                                                                                                                         >  1:160617/1‑251 (MQ=255)
    tttAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGTATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCAGTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATGTTGCCGCCCCTACCACCACTGCGATTGGCGCAACGTGCATCTTTATGTCTCTGCTTGTGGGGAtc                                                                                                                                                                                                                                      <  1:265819/250‑1 (MQ=255)
                                             ccgGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATTTTGCCGCCCCTACCACCACTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTGCTGGTGGGGCTCTCTGCCACCAGTTGGTCg                                                                                                                                                                                                                    >  1:111000/1‑227 (MQ=255)
                                                                         acacCGGCGCTCCGTTGAAGGCGGCGCTTCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAAGTTTGCCGCTCCTACCATCACGGCGAGTGGCGCAACGGGCAGCTTGATGTCGCTGCTGGTTGGGATCTCTGCCACCATGTGGTCGTATACCGGCATCTCCTCTATCTGTTATATGACCGGCGAAATGAAAAACCCCg                                                                                                                                                                <  1:54836/251‑1 (MQ=255)
                                                                                    ncGTTGAAGGCGGCGCAGCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATTTTGCCGCCCCTACCACCACTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTGCTGGTGGGGATCTCTGCCACCAGTTGGTCGTATACCGGCATGGCctc                                                                                                                                                                                                   >  1:63380/2‑205 (MQ=255)
                                                                                                               cGCTAATTACCATCGCCACAATTATCCCGTTCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATTATGCCGCCCCTACCACCACTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTGCTGGTGGGGATCTCTGCCACCAGTTGGTCGTATACCGGCATGGCCTCTATCTGTTATATGACCGGCGAAATTAAAAACCCCGGAAAAACCATGCCACGAGCGTTGATTGGTTCCTGTctg                                                                                                                          >  1:73539/1‑251 (MQ=255)
                                                                                                                                            ttCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATTTTGCCGCCACTACCACCACTGCGATTGGCGCAAAGGGCAGCTTTATGGCGCTGGTGGGGGGGATCTCTGCCACCAGTTGGACGTATACCGGCATGGCCTATATCTGTTATATGACCGGCGAAATTAAAAACCCCGGAAAAACCATGCCACGAGCGTTGATTGGTTCCTGTCTGCTGGTTCTGGTGCTCTACACCCTGCTGgc                                                                                             >  1:168052/1‑251 (MQ=255)
                                                                                                                                                                                                           accacTGCGATTGCCGCAACGGGAAGCTTTATGGCGCTGCTAGTGGGGATCCCTGCCACCAGTTTGTCGTATAACAGCATGGCCACTATCTGTTATATGACCGGCGAAATTAACAACCCCGGAAAAACAATGTCACGAGCGATGATTGGTTCCTGTCTGCTGGTTCTGGTGCTCTACACCATGTTGGCGCTGGTGATTTCCGGCCTGATGCCCTTCGACAAACTCGCCAATTCTGAAACGCCGATTTCCgc                              >  1:211578/1‑250 (MQ=255)
                                                                                                                                                                                                            ccacTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTNCTGCNGNNGANCNCGNccacc                                                                                                                                                                                                                             <  2:155330/59‑1 (MQ=255)
                                                                                                                                                                                                            ccacTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTGCTGGTGGGGATCTCTGccacc                                                                                                                                                                                                                             >  1:155330/1‑59 (MQ=255)
                                                                                                                                                                                                                                        tATGGCGCTGCTGGTGGGGATCTCTGCCACCAGTTGGTCGTATACCGGCATGGCCTCTATCTGGTATATGACCGGCGAAATTAAAAACCCCGGAAAAACCATGCCACGAGCGTTGATTGGTTCCTGTCTGCTGGTTCTGGTGCTCTACACCCTGCTGGCGCTGGTGATTTCCGGCCTGATGCCCTTCGACAAACTCGCCAATTCTGAAACGCCGATTTCCGACGCCCTGACCTGGATCCCCGCACTCGgc  <  1:261089/250‑1 (MQ=255)
                                                                                                                                                                                                                                             cgcTGCTGGTGGGGCTCTCTGCCACCAGTTGGTCGTATACCGGCATGGCCTCTATCTGTTATATg                                                                                                                                                                                      >  1:89488/1‑65 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                           
GCTTTTTAACGCCTATCGATCCGTTGCTCGGTAAATTTATCGCCGCCGGATTAATTATCGCCTTTATGTTGCTACACCTGCGCTCCGTTGAAGGCGGCGCAGCGTTTCAGACGCTAATTACCATCGCCAAAATTATCCCGTTCACTATCGTCATTGGCCTTGGGATCTTCTGGTTTAAAGCGGAGAATTTTGCCGCCCCTACCACCACTGCGATTGGCGCAACGGGCAGCTTTATGGCGCTGCTGGCGGGGATCTCTGCCACCAGTTGGTCGTATACCGGCATGGCCTCTATCTGTTATATGACCGGCGAAATTAAAAACCCCGGAAAAACCATGCCACGAGCGCTGATTGGTTCCTGTCTGCTGGTTCTGGTGCTCTACACCCTGCTGGCGCTGGTGATTTCCGGCCTGATGCCCTTCGACAAACTCGCCAATTCTGAAACGCCGATTTCCGACGCCCTGACCTGGATCCCCGCACTCGGC  >  NC_000913/3500259‑3500740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑