Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,291,951	T→C	I154V (ATT→GTT)	ccmH ←	holocytochrome c synthase CcmH component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,291,951	0	T	C	100.0%	22.6 / NA	12	I154V (ATT→GTT)	ccmH	holocytochrome c synthase CcmH component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/3);  total (9/3)

CCATGCCAACGCGGCCCAACATAATCCAGCCTTCTATATCTCCCGGATTTTTTTGCAGTTGAGTACGCATCCCCAGCGCAAGACGCGACATCTCTTCTTCGTTGAGCGGATCGGCTTTCGGATCCAGCGCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTT  >  NC_000913/2291711‑2292191                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 ncATGCCAACGCGGCCCAACATAATCCAGCCCTCTATATCTCCCGGATTTTTTTGCAGTTGAGTACGCATCCCCAGCGCAAGAAGAGACATCTCTTCTTCGTTGAACGAATCGCCTTTCGGCTCCAGCGCCCTGTCCAGCAACGCAGAAGCCTGTGCCGTGGCCTGCTGCCAGCTTTTCACCTGCTGATAATTGCTAGTCTGGTCGTAGCTGAAGCCAGCCCCAATTAACGCCACCACAACACACGGc                                                                                                                                                                                                                                           >  1:31194/2‑248 (MQ=255)  cATGCCAACGCGGCCCAACATAATCCAGCCTTCTATATCTCCCGGATTTTTTTGCAGTTGAGTACGCATCCCCAGCGCAAGACGCGACATCTCTTCTTCGTTGAGCGGCTCGGCTTTCGGATCCAGCGCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCCCCACAACACCCGGc                                                                                                                                                                                                                                           >  1:234307/1‑247 (MQ=255)  cATGCCAACGCGGCCCAACATAATCCAGCCTTCTATATCTCCCGGATTTTTTTGCAGTTGAGTACGCATCCCCAGCGCAAGACGCGACATCTCTTCTTCGTTGAGCGGATCGGCTTTCGGATCCAGCGCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAACACCCGGc                                                                                                                                                                                                                                           >  1:289566/1‑247 (MQ=255)  cATGCAAACGCCGACCACCATAATCAAGCCTTCTCTATCTCCCGGATTTTTTTGCAGTTGAGTACGCATCCCCAGCGCAAGACGCGACATCTCTTCTTCGTTGAGCGGATCGGCTTTCGGATCCAGCGCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAACACCCGGc                                                                                                                                                                                                                                           >  1:260803/1‑247 (MQ=255)                          ccAGCCTTCTATATCTCCCGGATTTTTTTGCAGTGGAGTACGCATCCCCAGCGCAAGACGCGACATCTCTTCTTCGTTGAGCGGATCGGCTTTCGGATCCAGCGCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTATCTGACGCCAGCCACAATGAACGCCACCACAACACCCGGCAGATAAACAACATATCCGGCACGCTTAc                                                                                                                                                                                                               <  1:321041/251‑1 (MQ=255)                                                                                                   ccccacggcgtgcgcggcggcGGATCCAGCGCCCTGTCCAGCAACGCCGGAGCCTGGGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAACCCCCGGCAGATAAACAACATAGCCGGCACGCTTACCTTCCGGCACGCTGTGTGCATGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGAc                                                                                                                                      <  1:55315/231‑1 (MQ=255)                                                                                                                         gATCCAGCGCCCTGACCAGCACCGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACATGCTGATAATTGCCAGTATGGTAGTAGATGACGACAGCCACAATTAACGCCACCACAACACGAGGCAGATAAACACCATATGCGGCTCGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGACAATGCCAATAGcc                                                                                                                >  1:319789/1‑251 (MQ=255)                                                                                                                             cAGCGCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAACACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTAcc                                                                                                                                                                >  1:88680/1‑199 (MQ=255)                                                                                                                                 gcCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTACCGCCACCACAACACCCGGCAGATAAACAACATATCCGGCACGc                                                                                                                                                                                                                   >  1:163780/1‑144 (MQ=255)                                                                                                                                                                    ctgctgCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGCCGCCAGCCACACTTACCGCCACCACAACACCCGGCCGATACACACCATATACGGCCCGCTTACCTTCCGGCACGCTTTGTTCCGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCACCCGCCAATGCCAATAGCCACTACTGGCCGCACACACAGCAGCACGGTCAGCGGCGTTAAcg                                                                     >  1:300200/1‑251 (MQ=255)                                                                                                                                                                                                  gCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAACGCCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTc                                                                                                                                                                                            <  1:184123/102‑1 (MQ=255)                                                                                                                                                                                                                           gccaCAATTAACGCCACCACAACACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGGGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAAtctc              >  1:317876/1‑251 (MQ=255)                                                                                                                                                                                                                                        ncaccaccacACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTttttactttt  >  1:83261/2‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                 CCATGCCAACGCGGCCCAACATAATCCAGCCTTCTATATCTCCCGGATTTTTTTGCAGTTGAGTACGCATCCCCAGCGCAAGACGCGACATCTCTTCTTCGTTGAGCGGATCGGCTTTCGGATCCAGCGCCCTGTCCAGCAACGCCGGAGCCTGTGCCGTGGCCTGCTGCCAGATTTTCACCTGCTGATAATTGCCAGTCTGGTAGTAGCTGACGCCAGCCACAATTAACGCCACCACAATACCCGGCAGATAAACAACATATCCGGCACGCTTACCTTCCGGCACGCTTTGTTCAGGAAACGCTTCCGGCACCACGCGTACCCGACGCCGCGAGCGGGCGTATATGACCCAACCGCCAATGCCAATAGCCACTACTGGCAGCACCCACAGCAGCACGGTCAGCGGCGTTAACGGCGGATCGTAAGTGACGAAGTTGCCGTAACGCGCCACCATATAATCGACAATCTCTTTTTTACTTTT  >  NC_000913/2291711‑2292191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑