Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,359,428	G→A	intergenic (‑87/+62)	ymjE ← / ← puuA	protein YmjE/glutamate‑putrescine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,359,428	0	G	A	100.0%	14.9 / NA	7	intergenic (‑87/+62)	ymjE/puuA	protein YmjE/glutamate‑putrescine ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/4);  total (3/4)

GTCGTGTCGACCTGAAATCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTGATAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTGATCATTCTCGATAAACTCACCCAGGGCATCGCTCTGGCGAATCGGAAAGGGTAAGCC  >  NC_000913/1359221‑1359672                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     gTCGTGTCGACCTGAAATCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGc                                                                                                                                                                                                                        <  1:48595/238‑1 (MQ=255)           ccTGAAATCAAACCTGTTTAGAAAGGGAACCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCg                                                                                                                                                                                                  >  1:86120/1‑250 (MQ=255)                              aGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCATGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTGCCTCGGCACAGTCTTAATCGTTAACGGTAGCTCTTTGCCGCCCGCGCTCCATAACGCGATATCCGGGAAATGGCTGAATTACCATCAAATGTCAGGGTTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGtttt                                                                                                                                                                              <  1:176820/251‑1 (MQ=255)                                                                        ngggCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGCCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCa                                                                                                                                     >  1:196997/2‑250 (MQ=255)                                                                                                                                                  tAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGTGTGTTTCAACATCCACTCAATTTCTGTTTCTGGGATGAGGCGCTTAAACTGCAACAGCGCATCATTTTTACAGGCGTGAGAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTg                                                           <  1:218920/250‑1 (MQ=255)                                                                                                                                                           aCGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCAGGTTTACAGGCGTGATAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTGATCATTCTCg                                                 <  1:300496/251‑1 (MQ=255)                                                                                                                                                                                                          tGTCTGAATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCAATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTGATAGAAATGGCAGAAGCGTTCGCCTAACTAGCGGCGCAGGTGATCATTCTCGATAAACTCACCCAGGGCATCGCTCTGGCGAATCGGAAAGGGTAAGcc  >  1:181714/1‑251 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                                                                     GTCGTGTCGACCTGAAATCAAACCTGTTTAGCAAGGGAAGCAAAGGGGGCGGAGTCCAGTCAGTAGCAGGAGGGGCGCATACGCAGCCTCCCTCGCCATGCGGGGATTTAATCATCGGCATCATCTCATTTCCTCGTCACAGTCTTAATCGTTAACGGAAGCCCGTTGCCGCCCGCGCTCCATAACGCGACATCCGGGAAATGTCTGGATTACCAGCAAATGTCAGGCCTGGCTCCGCTCAGGCCGATGAAACAACCCCGCAAGGGGTATTACGCGTTTTTCAACATCCACTCAATTTCTGTTTCTGTGATGAGGCGCTCAAACTGCAACAGCTCATCATTTTTACAGGCGTGATAGACATGGCAGAAGCGTTCGCCTAAATAGCGGCGCAGGTGATCATTCTCGATAAACTCACCCAGGGCATCGCTCTGGCGAATCGGAAAGGGTAAGCC  >  NC_000913/1359221‑1359672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑