Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,430,193	C→T	C110C (TGC→TGT)	cycA →	D‑serine/alanine/glycine/:H(+)symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,430,193	0	C	T	100.0%	31.6 / NA	13	C110C (TGC→TGT)	cycA	D‑serine/alanine/glycine/:H(+)symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/7);  total (6/7)

TGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTG  >  NC_000913/4429974‑4430388                                                                                                                                                                                                                            |                                                                                                                                                                                                    tGGTACGGGGTTGTTTATGGGGTTTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGAAGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACgtggtg                                                                                                                                                                       <  1:323443/250‑1 (MQ=255)                   ggggTTTGGCAAAACGAGTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTGCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGGGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATg                                                                                                                                                                                   <  1:46320/220‑1 (MQ=255)                          ggCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGCTTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTg                                                                                                                                             >  1:165518/1‑251 (MQ=255)                                                      cGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGc                                                                                                                  <  1:1029/250‑1 (MQ=255)                                                          cATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTGCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATGTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGAGCACTGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCg                                                                                                              <  1:183666/250‑1 (MQ=255)                                                                         gATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTTGAATCTGGAATACAAATCTTTTAGTGACGTCTCTTCCGATTTACTCGTGCCTTGGGCAGGATATTTCACCTGCTGTACTTACTGTTTCTGTTGGGTTGTAACCGTTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCg                                                                                                                   <  1:162153/230‑1 (MQ=255)                                                                                         ntctTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGCTTTACTCGGGACGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGGGCTGCTGCTGACGCTCa                                                                               >  1:63081/2‑250 (MQ=255)                                                                                                    gNTGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCCCCg                                                                   >  1:254465/1‑251 (MQ=255)                                                                                                                          cngctTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATTGTGCTGCTGCTGCAGCTCAATTTCGCCACCGTGGAAATGTTCGGTGGGATggg                                             >  1:253453/1‑250 (MQ=255)                                                                                                                                 ngAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGAGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCt                                                                                                                >  1:31074/2‑177 (MQ=255)                                                                                                                                                               tCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATtg          <  1:1642/249‑1 (MQ=255)                                                                                                                                                                     ccGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCCGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCCCCCCCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTg  >  1:245310/1‑251 (MQ=255)                                                                                                                                                                                         gCAGGATATTTCACCGGCTGGACTTACTGGTTCTGTTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCGCCGACTGGGTCGCCTCGCTGGCGGTGATAGTTCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGtt                                                         <  1:126251/176‑1 (MQ=255)                                                                                                                                                                                                                            |                                                                                                                                                                                                    TGGTACGGGGTTGTTTATGGGGTCTGGCAAAACGATTAGCCTTGCCGGGCCGTCGATCATTTTCGTTTATATGATCATTGGTTTTATGCTCTTTTTCGTGATGCGGGCAATGGGGGAATTGCTGCTTTCGAATCTGGAATACAAATCTTTTAGTGACTTCGCTTCCGATTTACTCGGGCCGTGGGCAGGATATTTCACCGGCTGGACTTACTGGTTCTGCTGGGTTGTAACCGGTATGGCAGACGTGGTGGCGATCACGGCTTATGCTCAGTTCTGGTTCCCCGATCTCTCCGACTGGGTCGCCTCGCTGGCGGTGATAGTGCTGCTGCTGACGCTCAATCTCGCCACCGTGAAAATGTTCGGTGAGATGGAGTTCTGGTTTGCGATGATCAAAATCGTCGCCATTGTGTCGCTG  >  NC_000913/4429974‑4430388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑