Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,751,127	C→T	A200T (GCT→ACT)	ydhW ←	uncharacterized protein YdhW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,751,127	0	C	T	100.0%	37.2 / NA	15	A200T (GCT→ACT)	ydhW	uncharacterized protein YdhW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/5);  total (10/5)

GTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGC  >  NC_000913/1750881‑1751359                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         gTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTCAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTg                                                                                                                                                                                                                                      >  1:172112/1‑251 (MQ=255)                                                                     ncAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTACTGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATg                                                                                                                                                                                     >  1:29552/2‑232 (MQ=255)                                                                            cAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGCTTATCCCCTAATGCAGTGGCTGCAACTGTCCACACTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCatat                                                                                                                                                           >  1:183925/1‑251 (MQ=255)                                                                                                 ncATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATACATCGCTCGCTAAACAAGTacaaca                                                                                                                                                                                                 >  1:191568/2‑192 (MQ=255)                                                                                                 ccATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACAGCTCCCTGAAAGTGCAGGGGTTCTGAAACTGTTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCa                                                                                                                                                                                                                                   <  1:131720/158‑1 (MQ=255)                                                                                                    tCCCCAGAACAAATTTGCGTCGAGTGAAGGACATATGATTATCCCCTAATGCAGAGGCTGCCACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGcc                                                                                                                                                                         >  1:287328/1‑213 (MQ=255)                                                                                                                           gTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTc                                                                                                            <  1:305251/251‑1 (MQ=255)                                                                                                                                                 cTNNNNNAGTNGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTacaaca                                                                                                                                                                                                 <  2:191568/144‑1 (MQ=255)                                                                                                                                                    aTGCAGTGGCTGCAACTGTCCACCCTATCCAGGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTCCCTCGAACCATTCGCATAAGCCATAAGTCTTGCCAGAGGTCATAAATCGCGCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGc                                                                                                                                                               <  1:186516/175‑1 (MQ=255)                                                                                                                                                                cAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCaa                                                                                                                                                 >  1:110311/1‑177 (MQ=255)                                                                                                                                                                                                             tCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAAtcg                                                                                                                                                                                                                     <  1:52079/64‑1 (MQ=255)                                                                                                                                                                                                             tCTGAAACTGCNCTCCCTCGAACCATTCGCATAAGCCATANGTCTTGCCATANGTCATNNNtnn                                                                                                                                                                                                                     >  2:52079/1‑62 (MQ=255)                                                                                                                                                                                                                       ctctACCTCGAACCATTCGCATAAGCCATAAGTCATGCCATAGGACATACCTCGATCGCTAACCAAGTACAACGCCGCCGTCGATGACTACACCTGCCGTATATCGGCATATTCAGGTGCACCATCCATcgc                                                                                                                                       >  1:292351/1‑132 (MQ=255)                                                                                                                                                                                                                                    cATTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTg   >  1:274226/1‑251 (MQ=255)                                                                                                                                                                                                                                     aTTCGCATAAGCCATAAGTCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCCTCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGCAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGc  >  1:153374/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         GTGACTCGTCATGAATCATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCACCCTATCCATGACATCTCCCTGAAATTTCAGGGGTTCTGAAACTGCTCTACCTCGAACCATTCGCATAAGCCATAAGCCTTGCCATAGGTCATAAATCGCTCGCTAAACAAGTACAACACCGCCGTCGATGACTCCACCTGCCGTATATCGGCATATTCAGGTGCAACATCCATCGCGGCGATCGCGGCTTCAATCTGCGCTTCCTGGAAGTAATATGGCGCTTGCATCAGCATGGCGACTTTGTAAGGACGAGGATACGTCTGGCACTCAAAACGAACGGCGTGTGCAATGGCGCGACAAATGTCCTGC  >  NC_000913/1750881‑1751359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑