Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,226,317	G→A	V173M (GTG→ATG)	yohD →	DedA family protein YohD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,226,317	0	G	A	100.0%	16.7 / NA	8	V173M (GTG→ATG)	yohD	DedA family protein YohD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/5);  total (3/5)

GCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTATTGGTTACGCTGGTGGTCAGGTGATTGCGCCGTGGTTGCACAATCTCGACCAGCATTTGAAGCACTGGGTCTGGTTGATTCTGGTCGTGGTTCTGGTGGTGGGCGTGCGCTGGTGGCTGAAACGACGCGGGAAGAAAAAGCCGGATCATCAGGCGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTG  >  NC_000913/2226133‑2226528                                                                                                                                                                                         |                                                                                                                                                                                                                    gCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTATTGGTTACGCTGGTGGTAAGGTGATTGCGCCGTGGTTGCACAATCTCGACCAGCATTTGAAGCACTGGGTCTGGTTGATTCTGGTCGTGGTTCTGATGGTGAGCGTgn                                                                                                                                                                                                         <  1:199517/197‑2 (MQ=255)                 cagcTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTATTGGTTACGCTGGTGGTCAGGTGATTGCGCCGTGGTTGCACAATCTCGACCAGCATTTGAAGCACTGGGTCTGGTTGATTCTGGTCATGGTTCTGATGGTGAGCGTGCGCTGGTGGCTGAAacg                                                                                                                                                                                         <  1:150710/197‑1 (MQ=255)                                                                     ggnNNNNGAANTTTTCCACTAGTGGTTACGCTGGTGGTCAGGTGATTGCGCCGTGGTTGCACAATCTCGACCAGCATTTGAAGCACTGGGGCTGGTTGATTCTGGTCGTGGTTCTGATGGTGAGCGTGCGCTGGTGGCTGAAac                                                                                                                                                                                          <  2:198643/144‑1 (MQ=255)                                                                                                 ctcTGGTGGTCAGGTGATTGCGCCGTGGTTGCACAATCTCGACCAGCATTTGAAGCACTGGGTCTGGTTGATTCTGGTCGTGGTTCTGATGGTGAGCGTGCGCTGGTGGCTGAAACGACGCGGGCAGAAAAAGCCGGATCATCAGGCGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCgg                                                                                                          >  1:312871/3‑196 (MQ=255)                                                                                                 cGCTGGTGGTCAGGTGATTGCGCCGTGGTTGCACAATCTCGACCAGCATTTGAAGCACTGGGTCTGGTTGATTCTGGTCGTGGTTCTGATGGTGAGCGTGCGCTGGTGGCTGAAACGACGCGGGAAGAAAAAGCCGGATCATCAGGCGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGAc                                                              >  1:160735/1‑240 (MQ=255)                                                                                                                            gTTGCACAATCTCGACCAGCATTTGAAGCGCGGGGTCTGGTTGATTCTGGTCGTGGTTCTGATGGTGAGCGTGCGCGGGTGGCTGAAACGACGCGGGAAGAAAAAGCCGGATCATCAGGCGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCAGGCCTGATACGACGCTGGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTg                        <  1:310461/251‑1 (MQ=255)                                                                                                                                cacaATCTCGACCAGCATTTGAAGCACTGGGTCTGGTTGATTCTGGTCGTGGTTCTGATGGCGAGCGTGCGCTGCTGGCTGAAACGCCGCGGGCAGAAAAAGACGGCTCATCAGGCGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCt                    >  1:304838/1‑251 (MQ=255)                                                                                                                                                   tGAAGCACTGGGTCTGGTTGATTCTGGTCGTGGTTCTGATGGTGAGCGTGCGCTGGTGGCTGAAACGTCGCGGGAAGAAAAAGCCGGATCATCAGGCGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGTAGTGCCATGCCTGATACGACGCTTGTGCGTCGTTTCATGCCGACCAAAAGATTACGCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACtgtg  <  1:302328/250‑1 (MQ=255)                                                                                                                                                                                         |                                                                                                                                                                                                                    GCTGATTGGTGCCAGCCAGCTGCCGCCGAAAATCTTTCTGCCGCTGAATATTCTCGGCGCATTTGCCTGGGCGTTGATTTTTACCACTATTGGTTACGCTGGTGGTCAGGTGATTGCGCCGTGGTTGCACAATCTCGACCAGCATTTGAAGCACTGGGTCTGGTTGATTCTGGTCGTGGTTCTGGTGGTGGGCGTGCGCTGGTGGCTGAAACGACGCGGGAAGAAAAAGCCGGATCATCAGGCGTAAAACCATTGCCCTGGATAAGGCGTTCGCGCCGCATCCGACATCCGGAGTACCATGCCTGATACGACGCTTGTGCGTCTTTTCATGCCGACCAAAATATTACCCAATGAAATATCCACGCACCTCACTGCGCTATTCTGGGTTGAACTGTG  >  NC_000913/2226133‑2226528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑