Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,440,203	C→T	G338D (GGT→GAT)	paeA ←	putative cadaverine/putrescine exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,440,203	0	C	T	100.0%	57.8 / NA	20	G338D (GGT→GAT)	paeA	putative cadaverine/putrescine exporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/8);  total (12/8)

CATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGCCGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCT  >  NC_000913/4440049‑4440427                                                                                                                                                           |                                                                                                                                                                                                                                 caTAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAAGCTGCTCTTCCAGCCCCTTATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATGGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCAc                                                                                                                                  <  1:221416/251‑1 (MQ=255)                   gccgATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACttt                                                                                                                >  1:22734/1‑251 (MQ=255)                   gccgATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGGCTTCACCTGCGGTTTTAAAACttt                                                                                                                <  1:105232/251‑1 (MQ=255)                                    ngCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCCGCCATGAGTTCTCATCACGGGCGCCAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACAGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAgg                                                                                               >  1:198156/2‑251 (MQ=255)                                                ggAACTCGTCACTATCCCGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATAAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGACAGGGTTAACGTATcc                                                                                   >  1:93183/1‑251 (MQ=255)                                                      cGTCAATATCCAGCACGCGCATGACGNCGTCAANNGGNGTGCCGCCGTCAATCAGCCNTGNNNTNNNANCACGGNCGNNAATCTGCTCNTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGNGTGATGATCNNCACCACCAGCGCGTACTCGTTNATGNNCNNCNCGANGt                                                                                                                                        >  2:70098/1‑192 (MQ=255)                                                                              cgtcgtCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAcg                                                     <  1:70098/251‑1 (MQ=255)                                                                               gtcgtcAATTGGTGTGCCACCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAcgc                                                    <  1:277845/251‑1 (MQ=255)                                                                               gtcgtcAATTGGGGTGCCACCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAcgc                                                    <  1:277849/251‑1 (MQ=255)                                                                                   tcAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTgg                                                                                                                                                                                                          >  1:156767/1‑97 (MQ=255)                                                                                         ggggTGCCGCCGTCAATCAGCCATGAGTTCTCATCCCGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTCCTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGCCGCTGTTc                                            >  1:105073/1‑249 (MQ=255)                                                                                              gccgccGTCAAGCATCCATGATTTCTCATCACGGTCGACAGTCTTCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCAc                                                                                                                                                                                                   <  1:301425/93‑1 (MQ=255)                                                                                                                      ctcATCACGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGCGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGCCGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGt             >  1:101482/1‑251 (MQ=255)                                                                                                                           caCGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATTACGTCATTGAGGGTGATGAGCCCCACCACCAGCGCGTACACGTTCATGATCACCGCGAAGTCTTCACCGGCGGTTTTAAAACTTTCCAACGCCTCGGAAAGGGTTAACGTATCCGGCACAATCATCTTGTTGCGAATTTGTACGCCGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTcag         <  1:322764/250‑1 (MQ=255)                                                                                                                            aCGGGCGACAATCTGCTCTTCCAGCCCCTGATCGACCCGATCGCCCATCAGCGTGGTCATCACGTCCTTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGCCGCTGTTCAGTGCCAGGCttt                               >  1:131366/1‑227 (MQ=255)                                                                                                                             ngggCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGCCGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTcagc        >  1:262548/2‑249 (MQ=255)                                                                                                                               ggCGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTAc                                                                                                                                                              >  1:257226/1‑97 (MQ=255)                                                                                                                                ncGACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTAcgccgc                                                 >  1:196309/2‑205 (MQ=255)                                                                                                                                 cNACAATCTGCTCTTCCAGCCCCTGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGCCGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCt  >  1:255791/1‑251 (MQ=255)                                                                                                                                                 cAGCCCCGGATCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTGCACCTGCGGTTTTAAAACGTTCCAACGCCTCTGAAAGGGTTAACGTAGCCGGCACAATCGGCGTGGTGCGAATTGGTACGCCGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTcagcag      <  1:107779/231‑1 (MQ=255)                                                                                                                                                           |                                                                                                                                                                                                                                 CATAAACATCATAAAGCCGCCGATGGTTTCGTAGTTGCCCGACTGCGGGAACTCGTCAATATCCAGCACGCGCATGACGTCGTCAATTGGGGTGCCGCCGTCAATCAGCCATGAGTTCTCATCACGGGCGACAATCTGCTCTTCCAGCCCCTGACCGACCAGATCGCCCATCAGCGTGGTCATCACGTCATTGAGGGTGATGATCCCCACCACCAGCGCGTACTCGTTCATGATCACCGCGAAGTCTTCACCTGCGGTTTTAAAACTTTCCAACGCCTCTGAAAGGGTTAACGTATCCGGCACAATCAGCGTGTTGCGAATTTGTACGCCGCTGTTCAGTGCCAGGCTTTGGTTAGCCAGCACGCGGTTCAGCAGGTCT  >  NC_000913/4440049‑4440427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑