Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,786,384	C→T	V243V (GTG→GTA)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,786,384	0	C	T	100.0%	30.2 / NA	12	V243V (GTG→GTA)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGCCCCATGCGGAAGTGATAAACACCACCTGGTCAAAGCCGGATTCGGTATCAATGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATA  >  NC_000913/1786139‑1786590                                                                                                                                                                                                                                                      |                                                                                                                                                                                                               ccATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGt                                                                                                                                                                                                            >  1:188603/1‑250 (MQ=255)                        cATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGTTAACCGCAc                                                                                                                                                                                    >  1:104504/1‑251 (MQ=255)                             ggCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGTTAACCGCACCCTgn                                                                                                                                                                               <  1:202701/251‑2 (MQ=255)                                         gtTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCAGTTTATGTACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCAtctc                                                                                                                                                                    >  1:21253/1‑250 (MQ=255)                                          tttCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTGGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGGCGTCGATGTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTATGTCGACCCCATGGTGCTGCGCACGTTGTGCTGACGATTCGCCGTCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGTTa                                                                                                                                                                                           <  1:158978/226‑1 (MQ=255)                                                                     naGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGCCCCATGCGGAAGTGATAAac                                                                                                                                        >  1:205224/2‑250 (MQ=255)                                                                                      cGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTTGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGTCCCCATGTGGAAGTGATAAACACCGCCTGGTCAAAGCCg                                                                                                                      <  1:275127/251‑1 (MQ=255)                                                                                                                                cTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGcc                                                                                                                                                           <  1:167253/172‑1 (MQ=255)                                                                                                                                                                   ggCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGGTAACCGCACCCTGCACGACCAtn                                                                                                                                                                      <  1:82883/126‑2 (MQ=255)                                                                                                                                                                        gTAAACCATGCGGATTTTTTGCTACCCCATGGTGCGGCGCACGATAGCCGGGCGATGCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGTTTAACCGCACCCTGCACGACCATCTCACCAAGTTCCGATTCGGAAGTGATAAACACCACCTGGTCAAAGCCGGATTCGGTATCAATGGAGAAc                                                                                                 <  1:186853/190‑1 (MQ=255)                                                                                                                                                                                                    nnggNNNNNCGCNCGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGCCCCATGCGGAAGTGATAAACACCACCTGGTCAAAGCCGGATTc                                                                                                                 <  2:205224/144‑1 (MQ=255)                                                                                                                                                                                                             cgcACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGTACGTAAAACTCCTCCGGGTTAACCGCCACCTGCCCGCCCATCTCACCAAGGACCCATGCGGAAGAGATAAACACCACCTGGTCAAAGCCGGATTCGGTATCAATGGAGAACATCACGCCAGCTGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCCCGGTGATCGTAACCCTGGTGCACACGATa  >  1:135747/1‑248 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                               CCATCTTTCGCCCACTCAATATCCATCGGGCGACCGTAGTGTTTCTCAATTTGTACGGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGCCCCATGCGGAAGTGATAAACACCACCTGGTCAAAGCCGGATTCGGTATCAATGGAGAACATCACGCCAGATGATGCGAGGTCAGAGCGCACCATCCGTTGAACACCGGCGGAGAGCGCCACACCACGGTGATCGTAACCCTGGTGCACACGATA  >  NC_000913/1786139‑1786590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑